(Circular) (Six-base) MAPSORT of: pFN21AE2635.seq Check: 3273 from: 1 to: 5875 pFN21AE2635 5875 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2256 2605 3938 5322 5590 1632 Size: 624 349 1333 1384 268 1917 Fragments arranged by size: 1917 1384 1333 624 349 268 AflII C'TTAA_G Cuts at: 820 1017 2696 820 Size: 197 1679 3999 Fragments arranged by size: 3999 1679 197 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2163 2195 2163 Size: 32 5843 AlwNI CAG_nnn'CTG Cuts at: 3618 5190 3618 Size: 1572 4303 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 2064 2198 2241 2318 2999 3184 3410 3562 Size: 571 134 43 77 681 185 226 152 Cuts at: 3562 5671 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 681 571 226 185 152 134 77 43 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2825 3200 1870 Size: 73 882 375 4545 Fragments arranged by size: 4545 882 375 73 BamHI G'GATC_C Cuts at: 3097 3205 3572 3097 Size: 108 367 5400 Fragments arranged by size: 5400 367 108 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2605 3196 4651 618 Size: 325 217 627 818 591 1455 1842 Fragments arranged by size: 1842 1455 818 627 591 325 217 BanII G_rGCy'C Cuts at: 1391 3194 1391 Size: 1803 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2825 928 Size: 971 926 3978 Fragments arranged by size: 3978 971 926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 3936 5092 1790 Size: 2146 1156 2573 Fragments arranged by size: 2573 2146 1156 BmgBI CAC'GTC Cuts at: 1318 2452 2899 1318 Size: 1134 447 4294 Fragments arranged by size: 4294 1134 447 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2190 4535 1239 Size: 951 2345 2579 Fragments arranged by size: 2579 2345 951 Bpu10I CC'TnA_GC Cuts at: 2520 2810 2520 Size: 290 5585 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2727 3123 4000 4865 5163 2727 Size: 396 877 865 298 3439 Fragments arranged by size: 3439 877 865 396 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 3123 5869 3123 Size: 2746 3129 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2163 2193 1178 Size: 30 165 30 760 30 4860 Fragments arranged by size: 4860 760 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5875 BseYI C'CCAG_C Cuts at: 5078 5574 5078 Size: 496 5379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5302 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3936 4021 5092 3936 Size: 85 1071 4719 Fragments arranged by size: 4719 1071 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3194 3936 4021 5092 1391 Size: 399 1404 742 85 1071 2174 Fragments arranged by size: 2174 1404 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 2529 3764 5494 2529 Size: 1235 1730 2910 Fragments arranged by size: 2910 1730 1235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2425 3230 844 Size: 1581 805 3489 Fragments arranged by size: 3489 1581 805 BsrBI CCG'CTC Cuts at: 1394 1823 2830 3762 1394 Size: 429 1007 932 3507 Fragments arranged by size: 3507 1007 932 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3088 4370 4544 65 Size: 1183 1840 1282 174 1396 Fragments arranged by size: 1840 1396 1282 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4519 4706 1700 Size: 110 2709 187 2869 Fragments arranged by size: 2869 2709 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 2669 794 Size: 1875 4000 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 2150 1184 Size: 966 4909 BstYI r'GATC_y Cuts at: 1972 3097 3205 3572 3957 3974 5426 5512 5524 Size: 1125 108 367 385 17 1452 86 12 Cuts at: 5524 5870 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1125 385 367 346 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2949 513 Size: 552 1884 3439 Fragments arranged by size: 3439 1884 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2667 3391 4185 4205 2667 Size: 724 794 20 4337 Fragments arranged by size: 4337 794 724 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 2323 3179 2323 Size: 856 5019 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2952 3322 4211 Size: 54 1425 50 275 18 1122 370 889 Cuts at: 4211 5840 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1122 889 370 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2402 3805 1353 Size: 1049 1403 3423 Fragments arranged by size: 3423 1403 1049 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2006 2675 4462 4836 4982 387 Size: 1064 138 417 669 1787 374 146 1280 Fragments arranged by size: 1787 1280 1064 669 417 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2256 2605 3938 4535 5322 5590 Size: 393 558 66 349 1333 597 787 268 Cuts at: 5590 1239 Size: 1524 Fragments arranged by size: 1524 1333 787 597 558 393 349 268 66 EcoO109I rG'GnC_Cy Cuts at: 2444 2524 2444 Size: 80 5795 EcoRI G'AATT_C Cuts at: 2064 3184 3562 2064 Size: 1120 378 4377 Fragments arranged by size: 4377 1120 378 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 2495 5022 1678 Size: 817 2527 2531 Fragments arranged by size: 2531 2527 817 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4516 4548 4590 4622 1373 Size: 32 135 32 2944 32 42 32 2626 Fragments arranged by size: 2944 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4989 5173 1305 Size: 3684 184 2007 Fragments arranged by size: 3684 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2954 10 Size: 54 1425 343 1122 2931 Fragments arranged by size: 2931 1425 1122 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 3031 3833 4192 4351 518 Size: 648 1320 545 802 359 159 2042 Fragments arranged by size: 2042 1320 802 648 545 359 159 MspA1I CmG'CkG Cuts at: 1606 3291 3968 5116 5361 1606 Size: 1685 677 1148 245 2120 Fragments arranged by size: 2120 1685 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2949 513 Size: 552 1884 3439 Fragments arranged by size: 3439 1884 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 3233 4778 1132 Size: 97 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PfoI T'CCnGG_A Cuts at: 2142 2142 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2163 2195 3944 3976 5457 5489 2163 Size: 32 1749 32 1481 32 2549 Fragments arranged by size: 2549 1749 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2890 1613 Size: 1277 4598 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspXI vC'TCGA_Gb Cuts at: 2825 2825 Size: 5875 PstI C_TGCA'G Cuts at: 830 2356 3227 830 Size: 1526 871 3478 Fragments arranged by size: 3478 1526 871 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 2352 2595 3223 4358 5039 5230 826 Size: 219 1307 243 628 1135 681 191 1471 Fragments arranged by size: 1471 1307 1135 681 628 243 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 2742 2825 3138 3979 4880 Size: 197 926 753 46 83 313 841 901 Cuts at: 4880 5142 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 841 753 313 262 197 83 46 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 2940 2949 3314 513 Size: 552 1875 9 365 3074 Fragments arranged by size: 3074 1875 552 365 9 SwaI ATTT'AAAT Cuts at: 2323 2323 Size: 5875 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2169 3139 3902 3919 4072 4257 4702 Size: 1255 589 970 763 17 153 185 445 Cuts at: 4702 325 Size: 1498 Fragments arranged by size: 1498 1255 970 763 589 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2261 2458 4121 Size: 274 80 33 51 1443 284 197 1663 Cuts at: 4121 5833 96 Size: 1712 138 Fragments arranged by size: 1712 1663 1443 284 274 197 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2121 2153 2321 2353 5835 5867 2121 Size: 32 168 32 3482 32 2129 Fragments arranged by size: 3482 2129 168 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2947 2989 2947 Size: 42 5833 XhoI C'TCGA_G Cuts at: 1943 2825 1943 Size: 882 4993 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 2615 4004 2615 Size: 1389 4486 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI