################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NarI NcoI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XhoII XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 1042 GTCTAC GT^[AC][GT]AC AcsI 917 AAATTC [GA]^AATT[CT] AflII 1054 CTTAAG C^TTAAG AflIII 331 ACATGT A^C[GA][CT]GT AocI 16 CCTAAGG CC^T[ACGT]AGG ApoI 917 AAATTC [GA]^AATT[CT] AspHI 490 GAGCAC G[AT]GC[AT]^C BfaI 133 CTAG C^TAG BfrI 1054 CTTAAG C^TTAAG BsaAI 1032 TACGTG [CT]AC^GT[GA] BsiEI 976 CGGCCG CG[GA][CT]^CG BsiHKAI 490 GAGCAC G[AT]GC[AT]^C BsmBI 238 GAGACG CGTCTC(1/5) Bsp1407I 644 TGTACA T^GTACA BspLU11I 331 ACATGT A^CATGT BsrFI 974 GCCGGC [GA]^CCGG[CT] BsrGI 644 TGTACA T^GTACA BstAPI 771 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 16 CCTAAGG CC^T[ACGT]AGG CfoI 244 GCGC GCG^C Cfr10I 974 GCCGGC [GA]^CCGG[CT] EagI 976 CGGCCG C^GGCCG EarI 123 GAAGAG CTCTTC(1/4) EclXI 976 CGGCCG C^GGCCG Eco52I 976 CGGCCG C^GGCCG Eco81I 16 CCTAAGG CC^T[ACGT]AGG FseI 973 GGCCGGCC GGCCGG^CC HaeII 243 GGCGCT [GA]GCGC^[CT] HgaI 1040 GCGTC GACGC(5/10) HhaI 244 GCGC GCG^C HinP1I 244 GCGC G^CGC HindIII 103 AAGCTT A^AGCTT Ksp632I 123 GAAGAG CTCTTC(1/4) MaeI 133 CTAG C^TAG MboII 123 GAAGA GAAGA(8/7) MseI 1055 TTAA T^TAA MslI 756 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 974 GCCGGC GCC^GGC NdeI 109 CATATG CA^TATG NgoMIV 974 GCCGGC G^CCGGC NspI 331 ACATGT [GA]CATG^[CT] PaeR7I 1085 CTCGAG C^TCGAG SexAI 392 ACCTGGT A^CC[AT]GGT SmaI 31 CCCGGG CCC^GGG SspBI 644 TGTACA T^GTACA Tru9I 1055 TTAA T^TAA Tsp45I 209 GTGAC ^GT[GC]AC Tsp509I 918 AATT ^AATT XbaI 132 TCTAGA T^CTAGA XcmI 798 CCAGTGGACGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1085 CTCGAG C^TCGAG XmaCI 31 CCCGGG C^CCGGG XmaI 31 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### ApaI 28 GGGCCC GGGCC^C 569 GGGCCC AvaI 31 CCCGGG C^[CT]CG[GA]G 1085 CTCGAG BcnI 31 CCCGG CC^[GC]GG 32 CCGGG BsaI 718 GGTCTC GGTCTC(1/5) 888 GGTCTC BsoBI 31 CCCGGG C^[CT]CG[GA]G 1085 CTCGAG DraII 27 AGGGCCC [GA]G^G[ACGT]CC[CT] 568 AGGGCCC EaeI 972 TGGCCG [CT]^GGCC[GA] 976 CGGCCG EcoO109I 27 AGGGCCC [GA]G^G[ACGT]CC[CT] 568 AGGGCCC EcoT14I 158 CCAAGG C^C[AT][AT]GG 284 CCTTGG FauI 413 CCCGC CCCGC(4/6) 467 CCCGC HapII 32 CCGG C^CGG 975 CCGG HpaII 32 CCGG C^CGG 975 CCGG MaeIII 209 GTGAC ^GT[ACGT]AC 752 GTAAC MspI 32 CCGG C^CGG 975 CCGG NciI 31 CCCGG CC^[GC]GG 32 CCGGG PflMI 90 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 915 CCAAATTCTGG PstI 115 CTGCAG CTGCA^G 685 CTGCAG SmlI 1054 CTTAAG C^T[CT][GA]AG 1085 CTCGAG StyI 158 CCAAGG C^C[AT][AT]GG 284 CCTTGG TfiI 129 GATTC G^A[AT]TC 1125 GATTC Van91I 90 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 915 CCAAATTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MboI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI TspRI TthHB8I