(Circular) (Six-base) MAPSORT of: pFN21AE5544.seq Check: 3726 from: 1 to: 5848 pFN21AE5544 5848 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2697 2697 Size: 5848 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3048 3191 1937 Size: 1111 143 4594 Fragments arranged by size: 4594 1111 143 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3911 5295 5563 1632 Size: 2279 1384 268 1917 Fragments arranged by size: 2279 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3059 820 Size: 197 2042 3609 Fragments arranged by size: 3609 2042 197 AflIII A'CryG_T Cuts at: 1203 2336 4747 5724 1203 Size: 1133 2411 977 1327 Fragments arranged by size: 2411 1327 1133 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5848 AlwNI CAG_nnn'CTG Cuts at: 2101 2453 2698 2779 3591 5163 2101 Size: 352 245 81 812 1572 2786 Fragments arranged by size: 2786 1572 812 352 245 81 ApaI G_GGCC'C Cuts at: 2037 2578 2037 Size: 541 5307 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 2922 3157 3383 3535 5644 1493 Size: 1429 235 226 152 2109 1697 Fragments arranged by size: 2109 1697 1429 235 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 2036 3090 3173 1870 Size: 73 93 1054 83 4545 Fragments arranged by size: 4545 1054 93 83 73 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3169 4624 618 Size: 325 217 627 1382 1455 1842 Fragments arranged by size: 1842 1455 1382 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 2236 2331 2578 3167 1391 Size: 646 199 95 247 589 4072 Fragments arranged by size: 4072 646 589 247 199 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4459 136 Size: 107 122 71 631 605 2787 1525 Fragments arranged by size: 2787 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 2037 2410 2578 2712 3909 5065 1790 Size: 247 373 168 134 1197 1156 2573 Fragments arranged by size: 2573 1197 1156 373 247 168 134 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2098 2933 3579 Size: 416 53 83 399 24 681 835 646 Cuts at: 3579 4537 442 Size: 958 1753 Fragments arranged by size: 1753 958 835 681 646 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2872 2872 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2603 2766 2903 3092 4508 1239 Size: 794 570 163 137 189 1416 2579 Fragments arranged by size: 2579 1416 794 570 189 163 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3973 4838 5136 3973 Size: 865 298 4685 Fragments arranged by size: 4685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2729 2899 4511 882 Size: 617 1230 170 1612 2219 Fragments arranged by size: 2219 1612 1230 617 170 BsaAI yAC'GTr Cuts at: 493 1204 3039 3639 493 Size: 711 1835 600 2702 Fragments arranged by size: 2702 1835 711 600 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3655 4037 4672 275 Size: 53 83 186 936 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5623 Fragments arranged by size: 5623 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2244 2546 2882 2887 3125 1186 Size: 1058 302 336 5 238 3909 Fragments arranged by size: 3909 1058 336 302 238 5 BseYI C'CCAG_C Cuts at: 5051 5547 5051 Size: 496 5352 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2191 2237 2346 2351 1163 Size: 573 455 46 109 5 4660 Fragments arranged by size: 4660 573 455 109 46 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2984 3298 4059 5087 1540 Size: 275 186 983 314 761 1028 2301 Fragments arranged by size: 2301 1028 983 761 314 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 3909 3994 5065 2499 Size: 1410 85 1071 3282 Fragments arranged by size: 3282 1410 1071 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 BsmBI CGTCTCn'nnnn_ Cuts at: 2237 2237 Size: 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2236 2331 2410 2499 2578 2712 Size: 399 247 199 95 79 89 79 134 Cuts at: 2712 3167 3909 3994 5065 1391 Size: 455 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 455 399 247 199 134 95 89 85 79 79 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2342 2664 2697 3021 3203 844 Size: 1498 322 33 324 182 3489 Fragments arranged by size: 3489 1498 324 322 182 33 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2979 4492 4679 1700 Size: 110 1169 1513 187 2869 Fragments arranged by size: 2869 1513 1169 187 110 BsrGI T'GTAC_A Cuts at: 96 2649 96 Size: 2553 3295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 2782 794 Size: 1988 3860 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstYI r'GATC_y Cuts at: 1972 3178 3545 3930 3947 5399 5485 5497 5843 Size: 1206 367 385 17 1452 86 12 346 Cuts at: 5843 1972 Size: 1977 Fragments arranged by size: 1977 1452 1206 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2022 2022 Size: 5848 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4357 Fragments arranged by size: 4357 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2356 487 Size: 683 56 1130 3979 Fragments arranged by size: 3979 1130 683 56 BtsI GCAGTG_nn' Cuts at: 2147 2363 2451 3364 4158 4178 2147 Size: 216 88 913 794 20 3817 Fragments arranged by size: 3817 913 794 216 88 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2977 2981 3295 Size: 54 1425 50 275 18 1147 4 314 Cuts at: 3295 4184 5813 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1147 889 314 275 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2981 3295 1537 Size: 275 1169 314 4090 Fragments arranged by size: 4090 1169 314 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2123 3778 1353 Size: 770 1655 3423 Fragments arranged by size: 3423 1655 770 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2450 4435 4809 4955 387 Size: 1064 138 861 1985 374 146 1280 Fragments arranged by size: 1985 1280 1064 861 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2603 2766 2903 3092 3911 4508 Size: 393 401 570 163 137 189 819 597 Cuts at: 4508 5295 5563 1239 Size: 787 268 1524 Fragments arranged by size: 1524 819 787 597 570 401 393 268 189 163 137 EcoO109I rG'GnC_Cy Cuts at: 2033 2574 2033 Size: 541 5307 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FseI GG_CCGG'CC Cuts at: 2983 2983 Size: 5848 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2252 4995 1678 Size: 574 2743 2531 Fragments arranged by size: 2743 2531 574 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2553 2585 2858 2890 4489 Size: 32 135 32 981 32 273 32 1599 Cuts at: 4489 4521 4563 4595 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1599 981 273 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3192 3438 669 Size: 988 281 1254 246 3079 Fragments arranged by size: 3079 1254 988 281 246 HindIII A'AGCT_T Cuts at: 748 2108 748 Size: 1360 4488 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4962 5146 1305 Size: 3657 184 2007 Fragments arranged by size: 3657 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4026 Fragments arranged by size: 4026 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2765 3806 4165 4324 518 Size: 648 1599 1041 359 159 2042 Fragments arranged by size: 2042 1599 1041 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3264 3941 5089 5334 1606 Size: 1658 677 1148 245 2120 Fragments arranged by size: 2120 1658 1148 677 245 NaeI GCC'GGC Cuts at: 2981 2981 Size: 5848 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4357 Fragments arranged by size: 4357 939 552 NdeI CA'TA_TG Cuts at: 387 2115 387 Size: 1728 4120 NgoMIV G'CCGG_C Cuts at: 2979 2979 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2340 3206 4751 1132 Size: 97 1111 866 1545 2229 Fragments arranged by size: 2229 1545 1111 866 97 PasI CC'CwG_GG Cuts at: 1348 2236 2587 1348 Size: 888 351 4609 Fragments arranged by size: 4609 888 351 PciI A'CATG_T Cuts at: 2336 4747 2336 Size: 2411 3437 PflMI CCAn_nnn'nTGG Cuts at: 1108 2101 2926 1108 Size: 993 825 4030 Fragments arranged by size: 4030 993 825 PfoI T'CCnGG_A Cuts at: 2880 2880 Size: 5848 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PspOMI G'GGCC_C Cuts at: 2033 2574 2033 Size: 541 5307 PstI C_TGCA'G Cuts at: 830 2124 2694 3200 830 Size: 1294 570 506 3478 Fragments arranged by size: 3478 1294 570 506 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SexAI A'CCwGG_T Cuts at: 2397 2397 Size: 5848 SfcI C'TryA_G Cuts at: 826 1045 2120 2690 3122 3196 4331 5012 5203 Size: 219 1075 570 432 74 1135 681 191 Cuts at: 5203 826 Size: 1471 Fragments arranged by size: 1471 1135 1075 681 570 432 219 191 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 2038 3175 1872 Size: 166 1137 4545 Fragments arranged by size: 4545 1137 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 3059 3090 3952 4853 5115 820 Size: 197 926 1116 31 862 901 262 1553 Fragments arranged by size: 1553 1116 926 901 862 262 197 31 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2004 2163 2289 3287 513 Size: 552 939 159 126 998 3074 Fragments arranged by size: 3074 998 939 552 159 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3875 3892 4045 4230 4675 325 Size: 1255 2295 17 153 185 445 1498 Fragments arranged by size: 2295 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2649 4094 5806 Size: 274 80 33 51 1443 672 1445 1712 Cuts at: 5806 96 Size: 138 Fragments arranged by size: 1712 1445 1443 672 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 5808 5840 2489 Size: 32 3287 32 2497 Fragments arranged by size: 3287 2497 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 2137 3184 2137 Size: 1047 4801 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2810 2810 Size: 5848 XhoI C'TCGA_G Cuts at: 1943 3090 1943 Size: 1147 4701 XmaI C'CCGG_G Cuts at: 1870 2036 3173 1870 Size: 166 1137 4545 Fragments arranged by size: 4545 1137 166 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI