(Linear) (Six-base) MAPSORT of: KIEE5544.seq Check: 3309 from: 1 to: 1143 KIEE5544.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 692 1143 Size: 692 451 AccI GT'mk_AC Cuts at: 0 1043 1143 Size: 1043 100 AflII C'TTAA_G Cuts at: 0 1054 1143 Size: 1054 89 AflIII A'CryG_T Cuts at: 0 331 1143 Size: 331 812 AlwNI CAG_nnn'CTG Cuts at: 0 96 448 693 774 1143 Size: 96 352 245 81 369 Fragments arranged by size: 369 352 245 96 81 ApaI G_GGCC'C Cuts at: 0 32 573 1143 Size: 32 541 570 Fragments arranged by size: 570 541 32 ApoI r'AATT_y Cuts at: 0 917 1143 Size: 917 226 AvaI C'yCGr_G Cuts at: 0 31 1085 1143 Size: 31 1054 58 Fragments arranged by size: 1054 58 31 BanII G_rGCy'C Cuts at: 0 32 231 326 573 1143 Size: 32 199 95 247 570 Fragments arranged by size: 570 247 199 95 32 Bme1580I G_kGCm'C Cuts at: 0 32 405 573 707 1143 Size: 32 373 168 134 436 Fragments arranged by size: 436 373 168 134 32 BmrI ACTGGGnnnn_n' Cuts at: 0 93 928 1143 Size: 93 835 215 Fragments arranged by size: 835 215 93 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 867 1143 Size: 867 276 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 598 761 898 1087 1143 Size: 28 570 163 137 189 56 Fragments arranged by size: 570 189 163 137 56 28 BsaI GGTCTCn'nnnn_ Cuts at: 0 724 894 1143 Size: 724 170 249 Fragments arranged by size: 724 249 170 BsaAI yAC'GTr Cuts at: 0 1034 1143 Size: 1034 109 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 239 541 877 882 1120 1143 Size: 239 302 336 5 238 23 Fragments arranged by size: 336 302 239 238 23 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 232 341 346 1143 Size: 186 46 109 5 797 Fragments arranged by size: 797 186 109 46 5 BsiEI CG_ry'CG Cuts at: 0 979 1143 Size: 979 164 BsiHKAI G_wGCw'C Cuts at: 0 494 1143 Size: 494 649 BsmBI CGTCTCn'nnnn_ Cuts at: 0 232 1143 Size: 232 911 Bsp1286I G_dGCh'C Cuts at: 0 32 231 326 405 494 573 707 1143 Size: 32 199 95 79 89 79 134 436 Fragments arranged by size: 436 199 134 95 89 79 79 32 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 337 659 692 1016 1143 Size: 337 322 33 324 127 Fragments arranged by size: 337 324 322 127 33 BsrFI r'CCGG_y Cuts at: 0 974 1143 Size: 974 169 BsrGI T'GTAC_A Cuts at: 0 644 1143 Size: 644 499 BstAPI GCAn_nnn'nTGC Cuts at: 0 777 1143 Size: 777 366 Bsu36I CC'TnA_GG Cuts at: 0 17 1143 Size: 17 1126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 351 1143 Size: 351 792 BtsI GCAGTG_nn' Cuts at: 0 142 358 446 1143 Size: 142 216 88 697 Fragments arranged by size: 697 216 142 88 EaeI y'GGCC_r Cuts at: 0 972 976 1143 Size: 972 4 167 Fragments arranged by size: 972 167 4 EagI C'GGCC_G Cuts at: 0 976 1143 Size: 976 167 EarI CTCTTCn'nnn_ Cuts at: 0 118 1143 Size: 118 1025 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 445 1143 Size: 445 698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 598 761 898 1087 1143 Size: 28 570 163 137 189 56 Fragments arranged by size: 570 189 163 137 56 28 EcoO109I rG'GnC_Cy Cuts at: 0 28 569 1143 Size: 28 541 574 Fragments arranged by size: 574 541 28 FseI GG_CCGG'CC Cuts at: 0 978 1143 Size: 978 165 HaeII r_GCGC'y Cuts at: 0 247 1143 Size: 247 896 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 548 580 853 885 1143 Size: 548 32 273 32 258 Fragments arranged by size: 548 273 258 32 32 HindIII A'AGCT_T Cuts at: 0 103 1143 Size: 103 1040 MslI CAynn'nnrTG Cuts at: 0 760 1143 Size: 760 383 NaeI GCC'GGC Cuts at: 0 976 1143 Size: 976 167 NdeI CA'TA_TG Cuts at: 0 110 1143 Size: 110 1033 NgoMIV G'CCGG_C Cuts at: 0 974 1143 Size: 974 169 NspI r_CATG'y Cuts at: 0 335 1143 Size: 335 808 PasI CC'CwG_GG Cuts at: 0 231 582 1143 Size: 231 351 561 Fragments arranged by size: 561 351 231 PciI A'CATG_T Cuts at: 0 331 1143 Size: 331 812 PflMI CCAn_nnn'nTGG Cuts at: 0 96 921 1143 Size: 96 825 222 Fragments arranged by size: 825 222 96 PfoI T'CCnGG_A Cuts at: 0 875 1143 Size: 875 268 PspOMI G'GGCC_C Cuts at: 0 28 569 1143 Size: 28 541 574 Fragments arranged by size: 574 541 28 PstI C_TGCA'G Cuts at: 0 119 689 1143 Size: 119 570 454 Fragments arranged by size: 570 454 119 SexAI A'CCwGG_T Cuts at: 0 392 1143 Size: 392 751 SfcI C'TryA_G Cuts at: 0 115 685 1117 1143 Size: 115 570 432 26 Fragments arranged by size: 570 432 115 26 SmaI CCC'GGG Cuts at: 0 33 1143 Size: 33 1110 SmlI C'TyrA_G Cuts at: 0 1054 1085 1143 Size: 1054 31 58 Fragments arranged by size: 1054 58 31 StyI C'CwwG_G Cuts at: 0 158 284 1143 Size: 158 126 859 Fragments arranged by size: 859 158 126 TatI w'GTAC_w Cuts at: 0 644 1143 Size: 644 499 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 484 516 1143 Size: 484 32 627 Fragments arranged by size: 627 484 32 XbaI T'CTAG_A Cuts at: 0 132 1143 Size: 132 1011 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 805 1143 Size: 805 338 XhoI C'TCGA_G Cuts at: 0 1085 1143 Size: 1085 58 XmaI C'CCGG_G Cuts at: 0 31 1143 Size: 31 1112 Enzymes that do cut: AarI AccI AflII AflIII AlwNI ApaI ApoI AvaI BanII Bme1580I BmrI BplI BpmI BsaI BsaAI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI BstAPI Bsu36I BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I FseI HaeII Hin4I HindIII MslI NaeI NdeI NgoMIV NspI PasI PciI PflMI PfoI PspOMI PstI SexAI SfcI SmaI SmlI StyI TatI TstI XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseYI BsiWI BsmI BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NarI NcoI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XmnI ZraI