################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AvaI AviII AvrII BanII BbrPI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MunI MvnI NaeI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 1537 GGTACC G^GTACC AcyI 3 GGCGCC G[GA]^CG[CT]C Asp718I 1537 GGTACC G^GTACC BamHI 1238 GGATCC G^GATCC BbeI 3 GGCGCC GGCGC^C BbvCI 1394 CCTCAGC CCTCAGC(-5/-2) BciVI 1711 GTATCC GTATCC(6/5) BsaHI 3 GGCGCC G[GA]^CG[CT]C BsgI 1517 GTGCAG GTGCAG(16/14) BsmI 1077 GAATGC GAATGC(1/-1) BstAPI 639 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1347 GGTGACC G^GT[ACGT]ACC BstPI 1347 GGTGACC G^GT[ACGT]ACC BstXI 1049 CCAGTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 4 GCGC GCG^C DrdI 1378 GACTATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1736 CCGTGG C^C[GA][CT]GG EcoO65I 1347 GGTGACC G^GT[ACGT]ACC HaeII 3 GGCGCC [GA]GCGC^[CT] HhaI 4 GCGC GCG^C Hin1I 3 GGCGCC G[GA]^CG[CT]C HinP1I 4 GCGC G^CGC KasI 3 GGCGCC G^GCGCC KpnI 1537 GGTACC GGTAC^C MaeII 683 ACGT A^CGT NarI 3 GGCGCC GG^CGCC NdeI 546 CATATG CA^TATG PshAI 895 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1570 CAGCTG CAG^CTG SfcI 171 CTACAG C^T[GA][CT]AG SfoI 3 GGCGCC GGC^GCC SspI 615 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Asp700I 223 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 892 GAAGACATTC AspHI 142 GTGCTC G[AT]GC[AT]^C 1466 GAGCAC AspI 592 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1605 GACGGTGTC BbsI 892 GAAGAC GAAGAC(2/6) 659 GTCTTC BbvI 639 GCAGC GCAGC(8/12) 1399 GCAGC BcnI 18 CCCGG CC^[GC]GG 600 CCGGG BpuAI 892 GAAGAC GAAGAC(2/6) 659 GTCTTC BsiHKAI 142 GTGCTC G[AT]GC[AT]^C 1466 GAGCAC BsmAI 578 GTCTC GTCTC(1/5) 939 GTCTC BspHI 216 TCATGA T^CATGA 1166 TCATGA BspMI 488 ACCTGC ACCTGC(4/8) 1727 ACCTGC BsrDI 294 CATTGC GCAATG(2/0) 1510 CATTGC EarI 227 CTCTTC CTCTTC(1/4) 52 GAAGAG FauI 90 CCCGC CCCGC(4/6) 1175 CCCGC Ksp632I 227 CTCTTC CTCTTC(1/4) 52 GAAGAG MslI 542 CACCCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1510 CATTGCAGTG MspA1I 91 CCGCTG C[AC]G^C[GT]G 1570 CAGCTG NciI 18 CCCGG CC^[GC]GG 600 CCGGG PflFI 592 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1605 GACGGTGTC RcaI 216 TCATGA T^CATGA 1166 TCATGA ScaI 1211 AGTACT AGT^ACT 1658 AGTACT SmlI 1365 CTTGAG C^T[CT][GA]AG 1419 CTTGAG TseI 639 GCAGC G^C[AT]GC 1399 GCAGC Tth111I 592 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1605 GACGGTGTC XcmI 830 CCATCCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1265 CCAATCTAGGCGTGG XmnI 223 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 892 GAAGACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AvaII BalI BanI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII