(Circular) (Six-base) MAPSORT of: pFN21AE5641.seq Check: 247 from: 1 to: 6454 pFN21AE5641 6454 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4264 278 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3797 1937 Size: 1860 4594 Acc65I G'GTAC_C Cuts at: 3542 3775 3542 Size: 233 6221 AclI AA'CG_TT Cuts at: 1563 4581 4954 1563 Size: 3018 373 3063 Fragments arranged by size: 3063 3018 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2519 2612 3625 4517 5901 6169 1632 Size: 710 177 93 1013 892 1384 268 1917 Fragments arranged by size: 1917 1384 1013 892 710 268 177 93 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6257 AflIII A'CryG_T Cuts at: 1203 2347 2436 2809 5353 6330 1203 Size: 1144 89 373 2544 977 1327 Fragments arranged by size: 2544 1327 1144 977 373 89 AgeI A'CCGG_T Cuts at: 5285 5285 Size: 6454 AhdI GACnn_n'nnGTC Cuts at: 5183 5183 Size: 6454 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3099 1184 Size: 1915 4539 AlwNI CAG_nnn'CTG Cuts at: 2365 2650 3451 4197 5769 2365 Size: 285 801 746 1572 3050 Fragments arranged by size: 3050 1572 801 746 285 ApaLI G'TGCA_C Cuts at: 4511 5667 4511 Size: 1156 5298 ApoI r'AATT_y Cuts at: 1493 2258 2713 3318 3763 3989 4141 6250 1493 Size: 765 455 605 445 226 152 2109 1697 Fragments arranged by size: 2109 1697 765 605 455 445 226 152 AseI AT'TA_AT Cuts at: 160 1032 5008 160 Size: 872 3976 1606 Fragments arranged by size: 3976 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 AvaI C'yCGr_G Cuts at: 1870 1943 3779 1870 Size: 73 1836 4545 Fragments arranged by size: 4545 1836 73 BamHI G'GATC_C Cuts at: 3243 3784 4151 3243 Size: 541 367 5546 Fragments arranged by size: 5546 541 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2545 3542 3590 3775 Size: 325 217 627 221 537 997 48 185 Cuts at: 3775 5230 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 997 627 537 325 221 217 185 48 BanII G_rGCy'C Cuts at: 1391 3773 1391 Size: 2382 4072 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 6454 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2657 2904 928 Size: 971 758 247 4478 Fragments arranged by size: 4478 971 758 247 BbvCI CC'TCA_GC Cuts at: 3400 3400 Size: 6454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4645 4679 1903 Size: 34 2708 34 3678 Fragments arranged by size: 3678 2708 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3727 4346 5556 3727 Size: 619 1210 4625 Fragments arranged by size: 4625 1210 619 BclI T'GATC_A Cuts at: 1548 1548 Size: 6454 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5065 136 Size: 107 122 71 631 605 3393 1525 Fragments arranged by size: 3393 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6449 6449 Size: 6454 BlpI GC'TnA_GC Cuts at: 3871 3871 Size: 6454 Bme1580I G_kGCm'C Cuts at: 1790 2548 2568 3494 4515 5671 1790 Size: 758 20 926 1021 1156 2573 Fragments arranged by size: 2573 1156 1021 926 758 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2806 4185 5143 Size: 416 53 83 399 24 1389 1379 958 Cuts at: 5143 442 Size: 1753 Fragments arranged by size: 1753 1389 1379 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6454 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2931 3599 3710 5114 1239 Size: 1692 668 111 1404 2579 Fragments arranged by size: 2579 1692 1404 668 111 Bpu10I CC'TnA_GC Cuts at: 3400 3495 3657 3400 Size: 95 162 6197 Fragments arranged by size: 6197 162 95 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3391 3445 4579 5444 5742 3391 Size: 54 1134 865 298 4103 Fragments arranged by size: 4103 1134 865 298 54 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5117 882 Size: 617 3618 2219 Fragments arranged by size: 3618 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4245 493 Size: 711 3041 2702 Fragments arranged by size: 3041 2702 711 BsaBI GATnn'nnATC Cuts at: 6448 6448 Size: 6454 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4261 4643 5278 Size: 53 83 186 936 476 2252 382 635 Cuts at: 5278 275 Size: 1451 Fragments arranged by size: 2252 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4887 5285 5559 5706 6117 4887 Size: 398 274 147 411 5224 Fragments arranged by size: 5224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3193 3223 1178 Size: 30 165 30 1790 30 4409 Fragments arranged by size: 4409 1790 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2045 2576 3596 1186 Size: 856 3 531 1020 4044 Fragments arranged by size: 4044 1020 856 531 3 BseYI C'CCAG_C Cuts at: 5657 6153 5657 Size: 496 5958 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3543 1163 Size: 573 1807 4074 Fragments arranged by size: 4074 1807 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3904 4665 5693 1540 Size: 275 186 1903 761 1028 2301 Fragments arranged by size: 2301 1903 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 3475 4515 4600 5671 2151 Size: 1324 1040 85 1071 2934 Fragments arranged by size: 2934 1324 1071 1040 85 BsmI GAATG_Cn' Cuts at: 3088 3965 4058 3088 Size: 877 93 5484 Fragments arranged by size: 5484 877 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2548 2568 3475 3494 3773 4515 Size: 399 361 397 20 907 19 279 742 Cuts at: 4515 4600 5671 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 907 742 399 397 361 279 85 20 19 BspEI T'CCGG_A Cuts at: 6117 6117 Size: 6454 BspHI T'CATG_A Cuts at: 2221 3171 4343 6073 2221 Size: 950 1172 1730 2602 Fragments arranged by size: 2602 1730 1172 950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2502 3741 3809 844 Size: 1658 1239 68 3489 Fragments arranged by size: 3489 1658 1239 68 BsrBI CCG'CTC Cuts at: 1394 1823 4341 1394 Size: 429 2518 3507 Fragments arranged by size: 3507 2518 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2298 3514 4949 5123 65 Size: 1183 1050 1216 1435 174 1396 Fragments arranged by size: 1435 1396 1216 1183 1050 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5098 5285 1700 Size: 110 3288 187 2869 Fragments arranged by size: 3288 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6454 BssSI C'ACGA_G Cuts at: 4514 5526 4514 Size: 1012 5442 BstAPI GCAn_nnn'nTGC Cuts at: 794 2650 794 Size: 1856 4598 BstBI TT'CG_AA Cuts at: 5258 5258 Size: 6454 BstEII G'GTnAC_C Cuts at: 1184 3352 1184 Size: 2168 4286 BstXI CCAn_nnnn'nTGG Cuts at: 3061 3061 Size: 6454 BstYI r'GATC_y Cuts at: 1972 2244 2470 3243 3784 4151 4536 4553 6005 Size: 272 226 773 541 367 385 17 1452 Cuts at: 6005 6091 6103 6449 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 773 541 385 367 346 272 226 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3741 513 Size: 552 939 1737 3226 Fragments arranged by size: 3226 1737 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5715 Fragments arranged by size: 5715 683 56 BtsI GCAGTG_nn' Cuts at: 3494 3526 3970 4764 4784 3494 Size: 32 444 794 20 5164 Fragments arranged by size: 5164 794 444 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6454 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3166 3201 529 Size: 35 2602 35 3782 Fragments arranged by size: 3782 2602 35 35 DraI TTT'AAA Cuts at: 3758 3758 Size: 6454 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3389 5461 809 Size: 507 2073 2072 1802 Fragments arranged by size: 2073 2072 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 3025 3211 Size: 54 1425 50 275 18 910 285 186 Cuts at: 3211 3377 3724 3738 3901 4790 6419 8 Size: 166 347 14 163 889 1629 43 Fragments arranged by size: 1629 1425 910 889 347 285 275 186 166 163 54 50 43 18 14 EagI C'GGCC_G Cuts at: 1537 1812 3901 1537 Size: 275 2089 4090 Fragments arranged by size: 4090 2089 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2238 4384 1353 Size: 699 186 2146 3423 Fragments arranged by size: 3423 2146 699 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5041 5415 5561 387 Size: 1064 138 3452 374 146 1280 Fragments arranged by size: 3452 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2519 2612 2931 3599 3625 3710 Size: 393 710 177 93 319 668 26 85 Cuts at: 3710 4517 5114 5901 6169 1239 Size: 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 710 668 597 393 319 268 177 93 85 26 EcoNI CCTnn'n_nnAGG Cuts at: 2498 2591 2729 2498 Size: 93 138 6223 Fragments arranged by size: 6223 138 93 EcoO109I rG'GnC_Cy Cuts at: 2281 2819 3104 3627 3636 2281 Size: 538 285 523 9 5099 Fragments arranged by size: 5099 538 523 285 9 EcoRI G'AATT_C Cuts at: 3763 4141 3763 Size: 378 6076 FspI TGC'GCA Cuts at: 4960 4960 Size: 6454 HaeII r_GCGC'y Cuts at: 1678 2012 5601 1678 Size: 334 3589 2531 Fragments arranged by size: 3589 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2786 2818 5095 5127 5169 Size: 32 135 32 1214 32 2277 32 42 Cuts at: 5169 5201 1373 Size: 32 2626 Fragments arranged by size: 2626 2277 1214 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3798 4044 669 Size: 988 281 1860 246 3079 Fragments arranged by size: 3079 1860 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6454 HpaI GTT'AAC Cuts at: 4044 4044 Size: 6454 KasI G'GCGC_C Cuts at: 2008 2008 Size: 6454 KpnI G_GTAC'C Cuts at: 3546 3779 3546 Size: 233 6221 MfeI C'AATT_G Cuts at: 4053 4053 Size: 6454 MluI A'CGCG_T Cuts at: 6330 6330 Size: 6454 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2368 5568 5752 1305 Size: 1063 3200 184 2007 Fragments arranged by size: 3200 2007 1063 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3027 3213 3379 3726 10 Size: 54 1425 343 1195 186 166 347 2738 Fragments arranged by size: 2738 1425 1195 347 343 186 166 54 MslI CAynn'nnrTG Cuts at: 518 1166 2551 3519 4412 4771 4930 518 Size: 648 1385 968 893 359 159 2042 Fragments arranged by size: 2042 1385 968 893 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 3577 3870 4547 5695 5940 1606 Size: 492 1479 293 677 1148 245 2120 Fragments arranged by size: 2120 1479 1148 677 492 293 245 NarI GG'CG_CC Cuts at: 2009 2009 Size: 6454 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4963 Fragments arranged by size: 4963 939 552 NdeI CA'TA_TG Cuts at: 387 2552 387 Size: 2165 4289 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6454 NotI GC'GGCC_GC Cuts at: 3901 3901 Size: 6454 NspI r_CATG'y Cuts at: 1132 1229 2351 2440 2813 3812 5357 1132 Size: 97 1122 89 373 999 1545 2229 Fragments arranged by size: 2229 1545 1122 999 373 97 89 PasI CC'CwG_GG Cuts at: 1348 2655 1348 Size: 1307 5147 PciI A'CATG_T Cuts at: 2347 2436 2809 5353 2347 Size: 89 373 2544 3448 Fragments arranged by size: 3448 2544 373 89 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 2845 3386 1108 Size: 1242 495 541 4176 Fragments arranged by size: 4176 1242 541 495 PfoI T'CCnGG_A Cuts at: 2604 2947 3687 2604 Size: 343 740 5371 Fragments arranged by size: 5371 740 343 PmeI GTTT'AAAC Cuts at: 3758 3758 Size: 6454 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4523 4555 6036 6068 4523 Size: 32 1481 32 4909 Fragments arranged by size: 4909 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2281 2819 3104 3627 2281 Size: 538 285 523 5108 Fragments arranged by size: 5108 538 523 285 PshAI GACnn'nnGTC Cuts at: 1613 2904 1613 Size: 1291 5163 PsiI TTA'TAA Cuts at: 3300 4024 3300 Size: 724 5730 PstI C_TGCA'G Cuts at: 830 3806 830 Size: 2976 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6454 PvuII CAG'CTG Cuts at: 3577 3577 Size: 6454 SalI G'TCGA_C Cuts at: 1936 3796 1936 Size: 1860 4594 SanDI GG'GwC_CC Cuts at: 2281 2281 Size: 6454 SbfI CC_TGCA'GG Cuts at: 3806 3806 Size: 6454 ScaI AGT'ACT Cuts at: 3218 3665 4702 6414 3218 Size: 447 1037 1712 3258 Fragments arranged by size: 3258 1712 1037 447 SfcI C'TryA_G Cuts at: 826 1045 2176 3802 4937 5618 5809 826 Size: 219 1131 1626 1135 681 191 1471 Fragments arranged by size: 1626 1471 1135 1131 681 219 191 SfoI GGC'GCC Cuts at: 2010 2010 Size: 6454 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6454 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6454 SmaI CCC'GGG Cuts at: 1872 3781 1872 Size: 1909 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3370 3424 4558 5459 5721 820 Size: 197 926 1427 54 1134 901 262 1553 Fragments arranged by size: 1553 1427 1134 926 901 262 197 54 SnaBI TAC'GTA Cuts at: 493 4245 493 Size: 3752 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6454 SphI G_CATG'C Cuts at: 1132 3812 1132 Size: 2680 3774 SspI AAT'ATT Cuts at: 5 52 2622 4378 5 Size: 47 2570 1756 2081 Fragments arranged by size: 2570 2081 1756 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6454 StyI C'CwwG_G Cuts at: 513 1065 2004 3893 513 Size: 552 939 1889 3074 Fragments arranged by size: 3074 1889 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2563 2669 2760 3517 4481 4498 4651 Size: 1255 983 106 91 757 964 17 153 Cuts at: 4651 4836 5281 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 983 964 757 445 185 153 106 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2082 2806 3216 Size: 274 80 33 51 1443 105 724 410 Cuts at: 3216 3663 4700 6412 96 Size: 447 1037 1712 138 Fragments arranged by size: 1712 1443 1037 724 447 410 274 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3088 3120 3193 3225 6414 6446 3088 Size: 32 73 32 3189 32 3096 Fragments arranged by size: 3189 3096 73 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2600 3613 1316 Size: 1284 1013 4157 Fragments arranged by size: 4157 1284 1013 XbaI T'CTAG_A Cuts at: 3790 3790 Size: 6454 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2842 3277 2842 Size: 435 6019 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6454 XmaI C'CCGG_G Cuts at: 1870 3779 1870 Size: 1909 4545 XmnI GAAnn'nnTTC Cuts at: 2232 2901 4583 2232 Size: 669 1682 4103 Fragments arranged by size: 4103 1682 669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4262 276 Size: 53 83 186 936 2728 2468 Fragments arranged by size: 2728 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI