(Linear) (Six-base) MAPSORT of: KIEE5641.seq Check: 1959 from: 1 to: 1749 KIEE5641.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. Acc65I G'GTAC_C Cuts at: 0 1537 1749 Size: 1537 212 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 514 607 1620 1749 Size: 337 177 93 1013 129 Fragments arranged by size: 1013 337 177 129 93 AflIII A'CryG_T Cuts at: 0 342 431 804 1749 Size: 342 89 373 945 Fragments arranged by size: 945 373 342 89 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1094 1749 Size: 1094 655 AlwNI CAG_nnn'CTG Cuts at: 0 360 645 1446 1749 Size: 360 285 801 303 Fragments arranged by size: 801 360 303 285 ApoI r'AATT_y Cuts at: 0 253 708 1313 1749 Size: 253 455 605 436 Fragments arranged by size: 605 455 436 253 BamHI G'GATC_C Cuts at: 0 1238 1749 Size: 1238 511 BanI G'GyrC_C Cuts at: 0 3 540 1537 1585 1749 Size: 3 537 997 48 164 Fragments arranged by size: 997 537 164 48 3 BbeI G_GCGC'C Cuts at: 0 7 1749 Size: 7 1742 BbsI GAAGACnn'nnnn_ Cuts at: 0 652 899 1749 Size: 652 247 850 Fragments arranged by size: 850 652 247 BbvCI CC'TCA_GC Cuts at: 0 1395 1749 Size: 1395 354 BciVI GTATCCnnnnn_n' Cuts at: 0 1722 1749 Size: 1722 27 Bme1580I G_kGCm'C Cuts at: 0 543 563 1489 1749 Size: 543 20 926 260 Fragments arranged by size: 926 543 260 20 BmrI ACTGGGnnnn_n' Cuts at: 0 801 1749 Size: 801 948 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 926 1594 1705 1749 Size: 926 668 111 44 Fragments arranged by size: 926 668 111 44 Bpu10I CC'TnA_GC Cuts at: 0 1395 1490 1652 1749 Size: 1395 95 162 97 Fragments arranged by size: 1395 162 97 95 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1386 1440 1749 Size: 1386 54 309 Fragments arranged by size: 1386 309 54 BsaHI Gr'CG_yC Cuts at: 0 4 1749 Size: 4 1745 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1188 1218 1749 Size: 1188 30 531 Fragments arranged by size: 1188 531 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 40 571 1591 1749 Size: 37 3 531 1020 158 Fragments arranged by size: 1020 531 158 37 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1538 1749 Size: 1538 211 BsiHKAI G_wGCw'C Cuts at: 0 146 1470 1749 Size: 146 1324 279 Fragments arranged by size: 1324 279 146 BsmI GAATG_Cn' Cuts at: 0 1083 1749 Size: 1083 666 Bsp1286I G_dGCh'C Cuts at: 0 146 543 563 1470 1489 1749 Size: 146 397 20 907 19 260 Fragments arranged by size: 907 397 260 146 20 19 BspHI T'CATG_A Cuts at: 0 216 1166 1749 Size: 216 950 583 Fragments arranged by size: 950 583 216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 497 1736 1749 Size: 497 1239 13 Fragments arranged by size: 1239 497 13 BsrDI GCAATG_nn' Cuts at: 0 293 1509 1749 Size: 293 1216 240 Fragments arranged by size: 1216 293 240 BstAPI GCAn_nnn'nTGC Cuts at: 0 645 1749 Size: 645 1104 BstEII G'GTnAC_C Cuts at: 0 1347 1749 Size: 1347 402 BstXI CCAn_nnnn'nTGG Cuts at: 0 1056 1749 Size: 1056 693 BstYI r'GATC_y Cuts at: 0 239 465 1238 1749 Size: 239 226 773 511 Fragments arranged by size: 773 511 239 226 BtgI C'CryG_G Cuts at: 0 1736 1749 Size: 1736 13 BtsI GCAGTG_nn' Cuts at: 0 1489 1521 1749 Size: 1489 32 228 Fragments arranged by size: 1489 228 32 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1161 1196 1749 Size: 1161 35 553 Fragments arranged by size: 1161 553 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1384 1749 Size: 1384 365 EaeI y'GGCC_r Cuts at: 0 735 1020 1206 1372 1719 1733 1749 Size: 735 285 186 166 347 14 16 Fragments arranged by size: 735 347 285 186 166 16 14 EarI CTCTTCn'nnn_ Cuts at: 0 47 233 1749 Size: 47 186 1516 Fragments arranged by size: 1516 186 47 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 514 607 926 1594 1620 1705 1749 Size: 337 177 93 319 668 26 85 44 Fragments arranged by size: 668 337 319 177 93 85 44 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 493 586 724 1749 Size: 493 93 138 1025 Fragments arranged by size: 1025 493 138 93 EcoO109I rG'GnC_Cy Cuts at: 0 276 814 1099 1622 1631 1749 Size: 276 538 285 523 9 118 Fragments arranged by size: 538 523 285 276 118 9 HaeII r_GCGC'y Cuts at: 0 7 1749 Size: 7 1742 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 781 813 1749 Size: 781 32 936 Fragments arranged by size: 936 781 32 KasI G'GCGC_C Cuts at: 0 3 1749 Size: 3 1746 KpnI G_GTAC'C Cuts at: 0 1541 1749 Size: 1541 208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 363 1749 Size: 363 1386 MscI TGG'CCA Cuts at: 0 1022 1208 1374 1721 1749 Size: 1022 186 166 347 28 Fragments arranged by size: 1022 347 186 166 28 MslI CAynn'nnrTG Cuts at: 0 546 1514 1749 Size: 546 968 235 Fragments arranged by size: 968 546 235 MspA1I CmG'CkG Cuts at: 0 93 1572 1749 Size: 93 1479 177 Fragments arranged by size: 1479 177 93 NarI GG'CG_CC Cuts at: 0 4 1749 Size: 4 1745 NdeI CA'TA_TG Cuts at: 0 547 1749 Size: 547 1202 NspI r_CATG'y Cuts at: 0 346 435 808 1749 Size: 346 89 373 941 Fragments arranged by size: 941 373 346 89 PasI CC'CwG_GG Cuts at: 0 650 1749 Size: 650 1099 PciI A'CATG_T Cuts at: 0 342 431 804 1749 Size: 342 89 373 945 Fragments arranged by size: 945 373 342 89 PflMI CCAn_nnn'nTGG Cuts at: 0 345 840 1381 1749 Size: 345 495 541 368 Fragments arranged by size: 541 495 368 345 PfoI T'CCnGG_A Cuts at: 0 599 942 1682 1749 Size: 599 343 740 67 Fragments arranged by size: 740 599 343 67 PpuMI rG'GwC_Cy Cuts at: 0 276 814 1099 1622 1749 Size: 276 538 285 523 127 Fragments arranged by size: 538 523 285 276 127 PshAI GACnn'nnGTC Cuts at: 0 899 1749 Size: 899 850 PsiI TTA'TAA Cuts at: 0 1295 1749 Size: 1295 454 PvuII CAG'CTG Cuts at: 0 1572 1749 Size: 1572 177 SanDI GG'GwC_CC Cuts at: 0 276 1749 Size: 276 1473 ScaI AGT'ACT Cuts at: 0 1213 1660 1749 Size: 1213 447 89 Fragments arranged by size: 1213 447 89 SfcI C'TryA_G Cuts at: 0 171 1749 Size: 171 1578 SfoI GGC'GCC Cuts at: 0 5 1749 Size: 5 1744 SmlI C'TyrA_G Cuts at: 0 1365 1419 1749 Size: 1365 54 330 Fragments arranged by size: 1365 330 54 SspI AAT'ATT Cuts at: 0 617 1749 Size: 617 1132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 558 664 755 1512 1749 Size: 558 106 91 757 237 Fragments arranged by size: 757 558 237 106 91 TatI w'GTAC_w Cuts at: 0 77 801 1211 1658 1749 Size: 77 724 410 447 91 Fragments arranged by size: 724 447 410 91 77 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1083 1115 1188 1220 1749 Size: 1083 32 73 32 529 Fragments arranged by size: 1083 529 73 32 32 Tth111I GACn'n_nGTC Cuts at: 0 595 1608 1749 Size: 595 1013 141 Fragments arranged by size: 1013 595 141 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 837 1272 1749 Size: 837 435 477 Fragments arranged by size: 837 477 435 XmnI GAAnn'nnTTC Cuts at: 0 227 896 1749 Size: 227 669 853 Fragments arranged by size: 853 669 227 Enzymes that do cut: Acc65I AcuI AflIII AlfI AlwNI ApoI BamHI BanI BbeI BbsI BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DrdI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NdeI NspI PasI PciI PflMI PfoI PpuMI PshAI PsiI PvuII SanDI ScaI SfcI SfoI SmlI SspI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanII BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI XbaI XhoI XmaI ZraI