################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CpoI DraI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MluI MroI MseI MslI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I TspRI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AfaI 627 GTAC GT^AC AspHI 325 GTGCTC G[AT]GC[AT]^C AvaI 108 CCCGGG C^[CT]CG[GA]G BalI 584 TGGCCA TGG^CCA BanI 407 GGCACC G^G[CT][GA]CC BanII 439 GAGCCC G[GA]GC[CT]^C BbvCI 333 CCTCAGC CCTCAGC(-5/-2) BfaI 436 CTAG C^TAG BglI 416 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 567 CTCCAG CTGGAG(16/14) BsiHKAI 325 GTGCTC G[AT]GC[AT]^C BsoBI 108 CCCGGG C^[CT]CG[GA]G BspDI 16 ATCGAT AT^CGAT BsrBI 221 GAGCGG CCGCTC(-3/-3) BsrFI 404 ACCGGC [GA]^CCGG[CT] BsrI 362 ACTGG ACTGG(1/-1) BstBI 143 TTCGAA TT^CGAA BstYI 398 GGATCT [GA]^GATC[CT] CfoI 384 GCGC GCG^C Cfr10I 404 ACCGGC [GA]^CCGG[CT] ClaI 16 ATCGAT AT^CGAT DraIII 342 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 226 GACACATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 584 TGGCCA [CT]^GGCC[GA] EcoRV 13 GATATC GAT^ATC EcoT14I 212 CCTTGG C^C[AT][AT]GG FauI 41 CCCGC CCCGC(4/6) HhaI 384 GCGC GCG^C HinP1I 384 GCGC G^CGC MaeI 436 CTAG C^TAG MaeII 557 ACGT A^CGT MaeIII 259 GTCAC ^GT[ACGT]AC MfeI 514 CAATTG C^AATTG MflI 398 GGATCT [GA]^GATC[CT] MluNI 584 TGGCCA TGG^CCA MscI 584 TGGCCA TGG^CCA MunI 514 CAATTG C^AATTG NlaIII 387 CATG CATG^ NspI 386 GCATGT [GA]CATG^[CT] NspV 143 TTCGAA TT^CGAA PflMI 90 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 474 GACTC GAGTC(4/5) RsaI 627 GTAC GT^AC SexAI 290 ACCTGGT A^CC[AT]GGT SfuI 143 TTCGAA TT^CGAA SmaI 108 CCCGGG CCC^GGG StyI 212 CCTTGG C^C[AT][AT]GG Tsp45I 259 GTCAC ^GT[GC]AC Van91I 90 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 398 GGATCT [GA]^GATC[CT] XmaCI 108 CCCGGG C^CCGGG XmaI 108 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 42 CCGC CCGC(-3/-1) 223 GCGG BbsI 235 GTCTTC GAAGAC(2/6) 253 GTCTTC BpuAI 235 GTCTTC GAAGAC(2/6) 253 GTCTTC BseRI 70 GAGGAG GAGGAG(10/8) 150 GAGGAG BsmAI 368 GAGAC GTCTC(1/5) 80 GTCTC BsmBI 79 CGTCTC CGTCTC(1/5) 368 GAGACG Cac8I 40 GCCCGC GC[ACGT]^[ACGT]GC 178 GCCAGC DdeI 311 CTGAG C^T[ACGT]AG 334 CTCAG EcoNI 63 CCTTTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 291 CCTGGTGGAGG HgaI 78 GCGTC GACGC(5/10) 618 GCGTC HphI 121 GGTGA GGTGA(8/7) 341 TCACC MboII 236 TCTTC GAAGA(8/7) 254 TCTTC PpuMI 104 GGGACCC [GA]G^G[AT]CC[CT] 186 GGGTCCC SfaNI 354 GCATC GCATC(5/9) 425 GCATC TfiI 19 GATTC G^A[AT]TC 509 GAATC Tsp509I 268 AATT ^AATT 515 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AluI AlwI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MnlI MspI MvaI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TaqI TseI TthHB8I