################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HpaI KasI KpnI Ksp632I MaeII MamI MflI MluI MluNI MroI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SmlI SnaBI SpeI SphI Sse8387I SwaI TfiI Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflIII 769 ACATGT A^C[GA][CT]GT AocI 489 CCTGAGG CC^T[ACGT]AGG Aor51HI 9 AGCGCT AGC^GCT ApaI 41 GGGCCC GGGCC^C Asp700I 430 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 194 GAGCAC G[AT]GC[AT]^C AvaI 483 CCCGGG C^[CT]CG[GA]G AvrII 930 CCTAGG C^CTAGG BanII 41 GGGCCC G[GA]GC[CT]^C BlnI 930 CCTAGG C^CTAGG BpmI 469 CTCCAG CTGGAG(16/14) BsaWI 903 TCCGGT [AT]^CCGG[AT] BseRI 355 CTCCTC GAGGAG(10/8) BsiHKAI 194 GAGCAC G[AT]GC[AT]^C BsmAI 318 GTCTC GTCTC(1/5) BsmI 704 GAATGC GAATGC(1/-1) BsoBI 483 CCCGGG C^[CT]CG[GA]G Bsp1407I 339 TGTACA T^GTACA BspLU11I 769 ACATGT A^CATGT BsrGI 339 TGTACA T^GTACA BstAPI 985 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 489 CCTGAGG CC^T[ACGT]AGG DsaI 45 CCGCGG C^C[GA][CT]GG Eco47III 9 AGCGCT AGC^GCT Eco81I 489 CCTGAGG CC^T[ACGT]AGG EcoNI 925 CCTTTCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 88 GCGTC GACGC(5/10) HindIII 406 AAGCTT A^AGCTT HinfI 316 GAGTC G^A[ACGT]TC HphI 261 TCACC GGTGA(8/7) KspI 45 CCGCGG CCGC^GG MfeI 716 CAATTG C^AATTG MunI 716 CAATTG C^AATTG PflMI 562 CCACATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 316 GAGTC GAGTC(4/5) SacII 45 CCGCGG CCGC^GG ScaI 365 AGTACT AGT^ACT SfaNI 1081 GCATC GCATC(5/9) SgrAI 57 CGCCGGTG C[GA]^CCGG[CT]G SmaI 483 CCCGGG CCC^GGG SspBI 339 TGTACA T^GTACA SspI 895 AATATT AAT^ATT StuI 465 AGGCCT AGG^CCT Tsp45I 766 GTGAC ^GT[GC]AC Van91I 562 CCACATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 483 CCCGGG C^CCGGG XmaI 483 CCCGGG C^CCGGG XmnI 430 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 46 CGCG CG^CG 87 CGCG AlwI 498 GATCC GGATC(4/5) 823 GATCC AvaII 906 GGTCC G^G[AT]CC 1213 GGACC BanI 3 GGTGCC G^G[CT][GA]CC 28 GGTGCC BfaI 931 CTAG C^TAG 973 CTAG BmyI 41 GGGCCC G[AGT]GC[ACT]^C 194 GAGCAC BsmFI 481 GTCCC GGGAC(10/14) 907 GTCCC Bsp1286I 41 GGGCCC G[AGT]GC[ACT]^C 194 GAGCAC BsrFI 58 GCCGGT [GA]^CCGG[CT] 917 GCCGGT BstNI 70 CCTGG CC^[AT]GG 609 CCAGG BstUI 46 CGCG CG^CG 87 CGCG CfoI 10 GCGC GCG^C 128 GCGC Cfr10I 58 GCCGGT [GA]^CCGG[CT] 917 GCCGGT DpnII 498 GATC ^GATC 823 GATC DraI 997 TTTAAA TTT^AAA 1318 TTTAAA DraII 449 AGGCCCT [GA]G^G[ACGT]CC[CT] 975 AGGGCCT EcoO109I 449 AGGCCCT [GA]G^G[ACGT]CC[CT] 975 AGGGCCT EcoRII 70 CCTGG ^CC[AT]GG 609 CCAGG EcoT14I 930 CCTAGG C^C[AT][AT]GG 949 CCAAGG HaeII 9 AGCGCT [GA]GCGC^[CT] 127 AGCGCC HhaI 10 GCGC GCG^C 128 GCGC HinP1I 10 GCGC G^CGC 128 GCGC MaeI 931 CTAG C^TAG 973 CTAG MboI 498 GATC ^GATC 823 GATC MboII 430 GAAGA GAAGA(8/7) 1152 TCTTC MslI 274 CATAACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1239 CATGAAAGTG MspA1I 45 CCGCGG C[AC]G^C[GT]G 106 CCGCTG MvaI 70 CCTGG CC^[AT]GG 609 CCAGG MvnI 46 CGCG CG^CG 87 CGCG NdeII 498 GATC ^GATC 823 GATC NspI 769 ACATGT [GA]CATG^[CT] 1123 GCATGT Sau3AI 498 GATC ^GATC 823 GATC StyI 930 CCTAGG C^C[AT][AT]GG 949 CCAAGG TaqI 750 TCGA T^CGA 828 TCGA TthHB8I 750 TCGA T^CGA 828 TCGA XcmI 618 CCAAGAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1034 CCACAGATGTTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BcnI BsaJI BsgI BsiYI BslI BspMI BsrI BssKI BstF5I Cac8I Cfr13I DdeI FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MaeIII MnlI MseI MspI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI Tru9I TseI Tsp509I TspRI