(Circular) (Six-base) MAPSORT of: pFN21AE2574.seq Check: 8611 from: 1 to: 6028 pFN21AE2574 6028 bp With 182 enzymes: SgfI * April 21, 2015 13:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2584 2621 3193 2584 Size: 37 572 5419 Fragments arranged by size: 5419 572 37 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3838 278 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3371 1937 Size: 1434 4594 Acc65I G'GTAC_C Cuts at: 3349 3349 Size: 6028 AclI AA'CG_TT Cuts at: 1563 4155 4528 1563 Size: 2592 373 3063 Fragments arranged by size: 3063 2592 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2435 3016 4091 5475 5743 1632 Size: 803 581 1075 1384 268 1917 Fragments arranged by size: 1917 1384 1075 803 581 268 AfeI AGC'GCT Cuts at: 2016 2016 Size: 6028 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5831 AflIII A'CryG_T Cuts at: 1203 2774 4927 5904 1203 Size: 1571 2153 977 1327 Fragments arranged by size: 2153 1571 1327 977 AgeI A'CCGG_T Cuts at: 4859 4859 Size: 6028 AhdI GACnn_n'nnGTC Cuts at: 4757 4757 Size: 6028 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6028 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6028 AlwNI CAG_nnn'CTG Cuts at: 3771 5343 3771 Size: 1572 4456 ApaI G_GGCC'C Cuts at: 2050 2050 Size: 6028 ApaLI G'TGCA_C Cuts at: 4085 5241 4085 Size: 1156 4872 ApoI r'AATT_y Cuts at: 1493 2304 2388 2439 2751 3094 3164 3337 3563 Size: 811 84 51 312 343 70 173 226 Cuts at: 3563 3715 5824 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 811 343 312 226 173 152 84 70 51 AseI AT'TA_AT Cuts at: 160 1032 4582 160 Size: 872 3550 1606 Fragments arranged by size: 3550 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 AvaI C'yCGr_G Cuts at: 1870 1943 2488 3353 1870 Size: 73 545 865 4545 Fragments arranged by size: 4545 865 545 73 AvrII C'CTAG_G Cuts at: 2935 2935 Size: 6028 BamHI G'GATC_C Cuts at: 3358 3725 3358 Size: 367 5661 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2033 3349 4804 618 Size: 325 217 627 221 25 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 325 221 217 25 BanII G_rGCy'C Cuts at: 1391 2050 3347 1391 Size: 659 1297 4072 Fragments arranged by size: 4072 1297 659 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5057 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4219 4253 1903 Size: 34 2282 34 3678 Fragments arranged by size: 3678 2282 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3920 5130 3920 Size: 1210 4818 BclI T'GATC_A Cuts at: 1548 1548 Size: 6028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4639 136 Size: 107 122 71 631 605 2967 1525 Fragments arranged by size: 2967 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6023 6023 Size: 6028 BlpI GC'TnA_GC Cuts at: 3445 3445 Size: 6028 Bme1580I G_kGCm'C Cuts at: 1790 2050 4089 5245 1790 Size: 260 2039 1156 2573 Fragments arranged by size: 2573 2039 1156 260 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6028 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3759 4717 442 Size: 416 53 83 399 24 2342 958 1753 Fragments arranged by size: 2342 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6028 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2459 4688 1239 Size: 1220 2229 2579 Fragments arranged by size: 2579 2229 1220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4153 5018 5316 4153 Size: 865 298 4865 Fragments arranged by size: 4865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4691 882 Size: 617 3192 2219 Fragments arranged by size: 3192 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3819 493 Size: 711 2615 2702 Fragments arranged by size: 2702 2615 711 BsaBI GATnn'nnATC Cuts at: 6022 6022 Size: 6028 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3835 4217 4852 275 Size: 53 83 186 936 2302 382 635 1451 Fragments arranged by size: 2302 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2908 4461 4859 5133 5280 5691 2908 Size: 1553 398 274 147 411 3245 Fragments arranged by size: 3245 1553 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5803 Fragments arranged by size: 5803 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 1186 Size: 1165 4863 BseYI C'CCAG_C Cuts at: 5231 5727 5231 Size: 496 5532 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2373 2799 2803 1163 Size: 573 637 426 4 4388 Fragments arranged by size: 4388 637 573 426 4 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3478 4239 5267 1540 Size: 275 186 1477 761 1028 2301 Fragments arranged by size: 2301 1477 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2203 4089 4174 5245 2203 Size: 1886 85 1071 2986 Fragments arranged by size: 2986 1886 1071 85 BsmI GAATG_Cn' Cuts at: 2715 3539 3632 2715 Size: 824 93 5111 Fragments arranged by size: 5111 824 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2203 3347 4089 4174 5245 1391 Size: 399 260 153 1144 742 85 1071 2174 Fragments arranged by size: 2174 1144 1071 742 399 260 153 85 BspEI T'CCGG_A Cuts at: 5691 5691 Size: 6028 BspHI T'CATG_A Cuts at: 3917 5647 3917 Size: 1730 4298 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2584 2621 2868 3193 3383 844 Size: 1740 37 247 325 190 3489 Fragments arranged by size: 3489 1740 325 247 190 37 BsrBI CCG'CTC Cuts at: 1394 1823 3915 1394 Size: 429 2092 3507 Fragments arranged by size: 3507 2092 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4523 4697 65 Size: 1183 3275 174 1396 Fragments arranged by size: 3275 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2063 2922 4672 4859 1700 Size: 110 253 859 1750 187 2869 Fragments arranged by size: 2869 1750 859 253 187 110 BsrGI T'GTAC_A Cuts at: 96 2344 96 Size: 2248 3780 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6028 BssSI C'ACGA_G Cuts at: 4088 5100 4088 Size: 1012 5016 BstAPI GCAn_nnn'nTGC Cuts at: 794 2996 794 Size: 2202 3826 BstBI TT'CG_AA Cuts at: 4832 4832 Size: 6028 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6028 BstYI r'GATC_y Cuts at: 1972 3358 3725 4110 4127 5579 5665 5677 6023 Size: 1386 367 385 17 1452 86 12 346 Cuts at: 6023 1972 Size: 1977 Fragments arranged by size: 1977 1452 1386 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2495 2495 Size: 6028 BtgI C'CryG_G Cuts at: 513 1065 2004 2050 513 Size: 552 939 46 4491 Fragments arranged by size: 4491 939 552 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5289 Fragments arranged by size: 5289 683 56 BtsI GCAGTG_nn' Cuts at: 2408 3544 4338 4358 2408 Size: 1136 794 20 4078 Fragments arranged by size: 4078 1136 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6028 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2386 2421 529 Size: 35 1822 35 4136 Fragments arranged by size: 4136 1822 35 35 DraI TTT'AAA Cuts at: 3004 3325 3332 3004 Size: 321 7 5700 Fragments arranged by size: 5700 321 7 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5035 809 Size: 507 3719 1802 Fragments arranged by size: 3719 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3475 4364 5993 Size: 54 1425 50 275 18 1645 889 1629 Cuts at: 5993 8 Size: 43 Fragments arranged by size: 1645 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3475 1537 Size: 275 1663 4090 Fragments arranged by size: 4090 1663 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3958 1353 Size: 2605 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2187 4615 4989 5135 387 Size: 1064 138 598 2428 374 146 1280 Fragments arranged by size: 2428 1280 1064 598 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2435 2459 3016 4091 4688 5475 5743 Size: 393 803 24 557 1075 597 787 268 Cuts at: 5743 1239 Size: 1524 Fragments arranged by size: 1524 1075 803 787 597 557 393 268 24 EcoNI CCTnn'n_nnAGG Cuts at: 2934 2934 Size: 6028 EcoO109I rG'GnC_Cy Cuts at: 2455 2981 2455 Size: 526 5502 EcoRI G'AATT_C Cuts at: 3337 3715 3337 Size: 378 5650 FspI TGC'GCA Cuts at: 4534 4534 Size: 6028 HaeII r_GCGC'y Cuts at: 1678 2018 2136 5175 1678 Size: 340 118 3039 2531 Fragments arranged by size: 3039 2531 340 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2190 2222 4669 4701 4743 Size: 32 135 32 618 32 2447 32 42 Cuts at: 4743 4775 1373 Size: 32 2626 Fragments arranged by size: 2626 2447 618 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3372 3618 669 Size: 988 281 1434 246 3079 Fragments arranged by size: 3079 1434 988 281 246 HindIII A'AGCT_T Cuts at: 748 2411 748 Size: 1663 4365 HpaI GTT'AAC Cuts at: 3618 3618 Size: 6028 KpnI G_GTAC'C Cuts at: 3353 3353 Size: 6028 MfeI C'AATT_G Cuts at: 2721 3627 2721 Size: 906 5122 MluI A'CGCG_T Cuts at: 5904 5904 Size: 6028 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5142 5326 1305 Size: 3837 184 2007 Fragments arranged by size: 3837 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4206 Fragments arranged by size: 4206 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2283 3248 3986 4345 4504 518 Size: 648 1117 965 738 359 159 2042 Fragments arranged by size: 2042 1117 965 738 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 2113 3444 4121 5269 5514 1606 Size: 446 61 1331 677 1148 245 2120 Fragments arranged by size: 2120 1331 1148 677 446 245 61 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4537 Fragments arranged by size: 4537 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6028 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6028 NotI GC'GGCC_GC Cuts at: 3475 3475 Size: 6028 NspI r_CATG'y Cuts at: 1132 1229 2778 3132 3386 4931 1132 Size: 97 1549 354 254 1545 2229 Fragments arranged by size: 2229 1549 1545 354 254 97 PasI CC'CwG_GG Cuts at: 1348 2075 1348 Size: 727 5301 PciI A'CATG_T Cuts at: 2774 4927 2774 Size: 2153 3875 PflMI CCAn_nnn'nTGG Cuts at: 1108 2573 1108 Size: 1465 4563 PmeI GTTT'AAAC Cuts at: 3332 3332 Size: 6028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4097 4129 5610 5642 4097 Size: 32 1481 32 4483 Fragments arranged by size: 4483 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6028 PsiI TTA'TAA Cuts at: 2313 2729 3598 2313 Size: 416 869 4743 Fragments arranged by size: 4743 869 416 PspOMI G'GGCC_C Cuts at: 2046 2046 Size: 6028 PstI C_TGCA'G Cuts at: 830 3380 830 Size: 2550 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6028 SacII CC_GC'GG Cuts at: 2053 2053 Size: 6028 SalI G'TCGA_C Cuts at: 1936 3370 1936 Size: 1434 4594 SbfI CC_TGCA'GG Cuts at: 3380 3380 Size: 6028 ScaI AGT'ACT Cuts at: 2372 4276 5988 2372 Size: 1904 1712 2412 Fragments arranged by size: 2412 1904 1712 SfcI C'TryA_G Cuts at: 826 1045 3376 4511 5192 5383 826 Size: 219 2331 1135 681 191 1471 Fragments arranged by size: 2331 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6028 SgrAI Cr'CCGG_yG Cuts at: 1700 2063 1700 Size: 363 5665 SmaI CCC'GGG Cuts at: 1872 2490 3355 1872 Size: 618 865 4545 Fragments arranged by size: 4545 865 618 SmlI C'TyrA_G Cuts at: 820 1017 1943 4132 5033 5295 820 Size: 197 926 2189 901 262 1553 Fragments arranged by size: 2189 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3819 493 Size: 3326 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6028 SphI G_CATG'C Cuts at: 1132 3386 1132 Size: 2254 3774 SspI AAT'ATT Cuts at: 5 52 2902 3952 5 Size: 47 2850 1050 2081 Fragments arranged by size: 2850 2081 1050 47 StuI AGG'CCT Cuts at: 1517 2472 1517 Size: 955 5073 StyI C'CwwG_G Cuts at: 513 1065 2004 2935 2954 3467 513 Size: 552 939 931 19 513 3074 Fragments arranged by size: 3074 939 931 552 513 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3235 4055 4072 4225 4410 4855 325 Size: 1255 1655 820 17 153 185 445 1498 Fragments arranged by size: 1655 1498 1255 820 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2344 2370 4274 Size: 274 80 33 51 1443 367 26 1904 Cuts at: 4274 5986 96 Size: 1712 138 Fragments arranged by size: 1904 1712 1443 367 274 138 80 51 33 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2812 2844 5988 6020 2812 Size: 32 3144 32 2820 Fragments arranged by size: 3144 2820 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6028 XbaI T'CTAG_A Cuts at: 3364 3364 Size: 6028 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2630 3046 2630 Size: 416 5612 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6028 XmaI C'CCGG_G Cuts at: 1870 2488 3353 1870 Size: 618 865 4545 Fragments arranged by size: 4545 865 618 XmnI GAAnn'nnTTC Cuts at: 2439 4157 2439 Size: 1718 4310 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3836 276 Size: 53 83 186 936 2302 2468 Fragments arranged by size: 2468 2302 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI