(Linear) (Six-base) MAPSORT of: KIEE2574.seq Check: 1569 from: 1 to: 1323 KIEE2574.orf With 182 enzymes: SgfI * April 21, 2015 11:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 579 616 1188 1323 Size: 579 37 572 135 Fragments arranged by size: 579 572 135 37 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 1011 1323 Size: 430 581 312 Fragments arranged by size: 581 430 312 AfeI AGC'GCT Cuts at: 0 11 1323 Size: 11 1312 AflIII A'CryG_T Cuts at: 0 769 1323 Size: 769 554 ApaI G_GGCC'C Cuts at: 0 45 1323 Size: 45 1278 ApoI r'AATT_y Cuts at: 0 299 383 434 746 1089 1159 1323 Size: 299 84 51 312 343 70 164 Fragments arranged by size: 343 312 299 164 84 70 51 AvaI C'yCGr_G Cuts at: 0 483 1323 Size: 483 840 AvrII C'CTAG_G Cuts at: 0 930 1323 Size: 930 393 BanI G'GyrC_C Cuts at: 0 3 28 1323 Size: 3 25 1295 Fragments arranged by size: 1295 25 3 BanII G_rGCy'C Cuts at: 0 45 1323 Size: 45 1278 Bme1580I G_kGCm'C Cuts at: 0 45 1323 Size: 45 1278 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 1323 Size: 454 869 BsaWI w'CCGG_w Cuts at: 0 903 1323 Size: 903 420 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 346 1323 Size: 346 977 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 368 794 798 1323 Size: 368 426 4 525 Fragments arranged by size: 525 426 368 4 BsiHKAI G_wGCw'C Cuts at: 0 198 1323 Size: 198 1125 BsmI GAATG_Cn' Cuts at: 0 710 1323 Size: 710 613 Bsp1286I G_dGCh'C Cuts at: 0 45 198 1323 Size: 45 153 1125 Fragments arranged by size: 1125 153 45 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 579 616 863 1188 1323 Size: 579 37 247 325 135 Fragments arranged by size: 579 325 247 135 37 BsrFI r'CCGG_y Cuts at: 0 58 917 1323 Size: 58 859 406 Fragments arranged by size: 859 406 58 BsrGI T'GTAC_A Cuts at: 0 339 1323 Size: 339 984 BstAPI GCAn_nnn'nTGC Cuts at: 0 991 1323 Size: 991 332 Bsu36I CC'TnA_GG Cuts at: 0 490 1323 Size: 490 833 BtgI C'CryG_G Cuts at: 0 45 1323 Size: 45 1278 BtsI GCAGTG_nn' Cuts at: 0 403 1323 Size: 403 920 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 381 416 1323 Size: 381 35 907 Fragments arranged by size: 907 381 35 DraI TTT'AAA Cuts at: 0 999 1320 1323 Size: 999 321 3 Fragments arranged by size: 999 321 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 182 1323 Size: 182 1141 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 430 454 1011 1323 Size: 430 24 557 312 Fragments arranged by size: 557 430 312 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 929 1323 Size: 929 394 EcoO109I rG'GnC_Cy Cuts at: 0 450 976 1323 Size: 450 526 347 Fragments arranged by size: 526 450 347 HaeII r_GCGC'y Cuts at: 0 13 131 1323 Size: 13 118 1192 Fragments arranged by size: 1192 118 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 185 217 1323 Size: 185 32 1106 Fragments arranged by size: 1106 185 32 HindIII A'AGCT_T Cuts at: 0 406 1323 Size: 406 917 MfeI C'AATT_G Cuts at: 0 716 1323 Size: 716 607 MslI CAynn'nnrTG Cuts at: 0 278 1243 1323 Size: 278 965 80 Fragments arranged by size: 965 278 80 MspA1I CmG'CkG Cuts at: 0 47 108 1323 Size: 47 61 1215 Fragments arranged by size: 1215 61 47 NspI r_CATG'y Cuts at: 0 773 1127 1323 Size: 773 354 196 Fragments arranged by size: 773 354 196 PasI CC'CwG_GG Cuts at: 0 70 1323 Size: 70 1253 PciI A'CATG_T Cuts at: 0 769 1323 Size: 769 554 PflMI CCAn_nnn'nTGG Cuts at: 0 568 1323 Size: 568 755 PsiI TTA'TAA Cuts at: 0 308 724 1323 Size: 308 416 599 Fragments arranged by size: 599 416 308 PspOMI G'GGCC_C Cuts at: 0 41 1323 Size: 41 1282 SacII CC_GC'GG Cuts at: 0 48 1323 Size: 48 1275 ScaI AGT'ACT Cuts at: 0 367 1323 Size: 367 956 SgrAI Cr'CCGG_yG Cuts at: 0 58 1323 Size: 58 1265 SmaI CCC'GGG Cuts at: 0 485 1323 Size: 485 838 SspI AAT'ATT Cuts at: 0 897 1323 Size: 897 426 StuI AGG'CCT Cuts at: 0 467 1323 Size: 467 856 StyI C'CwwG_G Cuts at: 0 930 949 1323 Size: 930 19 374 Fragments arranged by size: 930 374 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1230 1323 Size: 1230 93 TatI w'GTAC_w Cuts at: 0 339 365 1323 Size: 339 26 958 Fragments arranged by size: 958 339 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 807 839 1323 Size: 807 32 484 Fragments arranged by size: 807 484 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 625 1041 1323 Size: 625 416 282 Fragments arranged by size: 625 416 282 XmaI C'CCGG_G Cuts at: 0 483 1323 Size: 483 840 XmnI GAAnn'nnTTC Cuts at: 0 434 1323 Size: 434 889 Enzymes that do cut: AarI AcuI AfeI AflIII ApaI ApoI AvaI AvrII BanI BanII Bme1580I BpmI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI BstAPI Bsu36I BtgI BtsI CspCI DraI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MfeI MslI MspA1I NspI PasI PciI PflMI PsiI PspOMI SacII ScaI SgrAI SmaI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI DraIII DrdI EaeI EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI SmlI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI ZraI