################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI ClaI CpoI DraI DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfaNI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI StuI StyI SwaI Tru9I Tth111I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 917 GAATTC [GA]^AATT[CT] AfaI 432 GTAC GT^AC AhdI 647 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 940 CCTGAGG CC^T[ACGT]AGG ApoI 917 GAATTC [GA]^AATT[CT] AspEI 647 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 130 GAGCTC G[AT]GC[AT]^C BbsI 331 GAAGAC GAAGAC(2/6) BfaI 733 CTAG C^TAG BpuAI 331 GAAGAC GAAGAC(2/6) BsaI 295 GAGACC GGTCTC(1/5) BseRI 1049 GAGGAG GAGGAG(10/8) BsgI 790 GTGCAG GTGCAG(16/14) BsiHKAI 130 GAGCTC G[AT]GC[AT]^C BsmBI 783 GAGACG CGTCTC(1/5) BsmI 109 GAATGC GAATGC(1/-1) Bsp1407I 431 TGTACA T^GTACA BsrBI 769 GAGCGG CCGCTC(-3/-3) BsrGI 431 TGTACA T^GTACA BssSI 1258 CACGAG CACGAG(-5/-1) Bsu36I 940 CCTGAGG CC^T[ACGT]AGG DsaI 822 CCACGG C^C[GA][CT]GG Eam1105I 647 GACAGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 940 CCTGAGG CC^T[ACGT]AGG EcoRI 917 GAATTC G^AATTC HindIII 899 AAGCTT A^AGCTT MaeI 733 CTAG C^TAG NaeI 889 GCCGGC GCC^GGC NgoMIV 889 GCCGGC G^CCGGC NspI 276 ACATGC [GA]CATG^[CT] RsaI 432 GTAC GT^AC SacI 130 GAGCTC GAGCT^C SbfI 54 CCTGCAGG CCTGCA^GG SmaI 21 CCCGGG CCC^GGG Sse8387I 54 CCTGCAGG CCTGCA^GG SspBI 431 TGTACA T^GTACA XmaCI 21 CCCGGG C^CCGGG XmaI 21 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 826 GGCGTC G[GA]^CG[CT]C 991 GACGCC AlwI 214 GATCC GGATC(4/5) 739 GATCC BanI 449 GGCACC G^G[CT][GA]CC 702 GGCACC BcgI 225 CGAGGGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 837 CGAGGGCCCTGC BpmI 657 CTCCAG CTGGAG(16/14) 1064 CTGGAG BsaHI 826 GGCGTC G[GA]^CG[CT]C 991 GACGCC BspMI 57 GCAGGT ACCTGC(4/8) 1090 GCAGGT BsrDI 597 CATTGC GCAATG(2/0) 806 GCAATG BsrFI 676 GCCGGT [GA]^CCGG[CT] 889 GCCGGC Cfr10I 676 GCCGGT [GA]^CCGG[CT] 889 GCCGGC EarI 441 GAAGAG CTCTTC(1/4) 515 GAAGAG FauI 1102 CCCGC CCCGC(4/6) 206 GCGGG Hin1I 826 GGCGTC G[GA]^CG[CT]C 991 GACGCC Ksp632I 441 GAAGAG CTCTTC(1/4) 515 GAAGAG MaeIII 1124 GTCAC ^GT[ACGT]AC 1214 GTCAC MslI 759 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 802 CACAGCAATG PaeR7I 985 CTCGAG C^TCGAG 1133 CTCGAG PpuMI 228 GGGTCCT [GA]G^G[AT]CC[CT] 727 GGGACCC SapI 441 GAAGAGC GCTCTTC(1/4) 515 GAAGAGC SexAI 193 ACCTGGT A^CC[AT]GGT 705 ACCAGGT SmlI 985 CTCGAG C^T[CT][GA]AG 1133 CTCGAG Tsp45I 1124 GTCAC ^GT[GC]AC 1214 GTCAC TspRI 392 CACTG CA[GC]TG[ACGT][ACGT]^ 882 CACTG XcmI 191 CCACCTGGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 849 CCACATCCCCCCTGG XhoI 985 CTCGAG C^TCGAG 1133 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AluI AlwNI ApaI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DraIII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PshAI PstI PvuII Sau3AI Sau96I ScrFI SfcI TaqI TfiI TseI Tsp509I TthHB8I Van91I