(Circular) (Six-base) MAPSORT of: pFN21AE5554.seq Check: 1731 from: 1 to: 5992 pFN21AE5554 5992 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2053 2053 Size: 5992 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2244 4055 5439 5707 1632 Size: 612 1811 1384 268 1917 Fragments arranged by size: 1917 1811 1384 612 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 4891 5868 1203 Size: 3688 977 1327 Fragments arranged by size: 3688 1327 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 2657 4721 2657 Size: 2064 3928 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2040 2072 2040 Size: 32 5960 AlwNI CAG_nnn'CTG Cuts at: 2068 2078 2882 3068 3735 5307 2068 Size: 10 804 186 667 1572 2753 Fragments arranged by size: 2753 1572 804 667 186 10 ApaI G_GGCC'C Cuts at: 2380 2757 2849 2380 Size: 377 92 5523 Fragments arranged by size: 5523 377 92 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 2922 3301 3527 3679 5788 1493 Size: 1429 379 226 152 2109 1697 Fragments arranged by size: 2109 1697 1429 379 226 152 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 2026 2990 3138 3317 1870 Size: 73 83 964 148 179 4545 Fragments arranged by size: 4545 964 179 148 83 73 BamHI G'GATC_C Cuts at: 3322 3689 3322 Size: 367 5625 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2454 2707 3313 4768 618 Size: 325 217 627 667 253 606 1455 1842 Fragments arranged by size: 1842 1455 667 627 606 325 253 217 BanII G_rGCy'C Cuts at: 1391 2139 2380 2757 2849 3311 1391 Size: 748 241 377 92 462 4072 Fragments arranged by size: 4072 748 462 377 241 92 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2343 928 Size: 971 444 4577 Fragments arranged by size: 4577 971 444 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2219 2253 2831 2865 4183 4217 1903 Size: 34 282 34 578 34 1318 34 3678 Fragments arranged by size: 3678 1318 578 282 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4603 136 Size: 107 122 71 631 605 2931 1525 Fragments arranged by size: 2931 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 3409 3409 Size: 5992 Bme1580I G_kGCm'C Cuts at: 1790 2380 2757 2849 4053 5209 1790 Size: 590 377 92 1204 1156 2573 Fragments arranged by size: 2573 1204 1156 590 377 92 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5992 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2408 2870 2922 Size: 416 53 83 399 24 991 462 52 Cuts at: 2922 3723 4681 442 Size: 801 958 1753 Fragments arranged by size: 1753 991 958 801 462 416 399 83 53 52 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2647 3090 4652 1239 Size: 1408 443 1562 2579 Fragments arranged by size: 2579 1562 1408 443 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4117 4982 5280 4117 Size: 865 298 4829 Fragments arranged by size: 4829 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2294 4655 882 Size: 617 795 2361 2219 Fragments arranged by size: 2361 2219 795 617 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2832 2997 3799 4181 Size: 53 83 186 936 1299 165 802 382 Cuts at: 4181 4816 275 Size: 635 1451 Fragments arranged by size: 1451 1299 936 802 635 382 186 165 83 53 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2679 2709 1178 Size: 30 165 30 1276 30 4461 Fragments arranged by size: 4461 1276 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3069 1186 Size: 1883 4109 BseYI C'CCAG_C Cuts at: 2100 2274 2800 3164 5195 5691 2100 Size: 174 526 364 2031 496 2401 Fragments arranged by size: 2401 2031 526 496 364 174 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2816 1163 Size: 573 1080 4339 Fragments arranged by size: 4339 1080 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3442 4203 5231 1540 Size: 275 186 1441 761 1028 2301 Fragments arranged by size: 2301 1441 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 4053 4138 5209 2139 Size: 1914 85 1071 2922 Fragments arranged by size: 2922 1914 1071 85 BsmI GAATG_Cn' Cuts at: 2120 3503 3596 2120 Size: 1383 93 4516 Fragments arranged by size: 4516 1383 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2782 2782 Size: 5992 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2380 2757 2849 3311 4053 4138 Size: 399 349 241 377 92 462 742 85 Cuts at: 4138 5209 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 462 399 377 349 241 92 85 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 3881 5611 3881 Size: 1730 4262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 3086 3347 844 Size: 1209 1033 261 3489 Fragments arranged by size: 3489 1209 1033 261 BsrBI CCG'CTC Cuts at: 1394 1823 2776 3879 1394 Size: 429 953 1103 3507 Fragments arranged by size: 3507 1103 953 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2601 2818 4487 4661 65 Size: 1183 1353 217 1669 174 1396 Fragments arranged by size: 1669 1396 1353 1183 217 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2681 2894 4636 4823 1700 Size: 110 871 213 1742 187 2869 Fragments arranged by size: 2869 1742 871 213 187 110 BsrGI T'GTAC_A Cuts at: 96 2436 96 Size: 2340 3652 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5992 BssSI C'ACGA_G Cuts at: 3263 4052 5064 3263 Size: 789 1012 4191 Fragments arranged by size: 4191 1012 789 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5992 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstYI r'GATC_y Cuts at: 1972 3322 3689 4074 4091 5543 5629 5641 5987 Size: 1350 367 385 17 1452 86 12 346 Cuts at: 5987 1972 Size: 1977 Fragments arranged by size: 1977 1452 1350 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2946 2946 Size: 5992 BtgI C'CryG_G Cuts at: 513 1065 2004 2827 513 Size: 552 939 823 3678 Fragments arranged by size: 3678 939 823 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2371 487 Size: 683 56 1145 4108 Fragments arranged by size: 4108 1145 683 56 BtsI GCAGTG_nn' Cuts at: 2886 3508 4302 4322 2886 Size: 622 794 20 4556 Fragments arranged by size: 4556 794 622 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DraIII CAC_nnn'GTG Cuts at: 2202 2714 3247 2202 Size: 512 533 4947 Fragments arranged by size: 4947 533 512 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3439 4328 5957 Size: 54 1425 50 275 18 1609 889 1629 Cuts at: 5957 8 Size: 43 Fragments arranged by size: 1629 1609 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3439 1537 Size: 275 1627 4090 Fragments arranged by size: 4090 1627 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2441 2515 3922 1353 Size: 1088 74 1407 3423 Fragments arranged by size: 3423 1407 1088 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2668 4579 4953 5099 387 Size: 1064 138 1079 1911 374 146 1280 Fragments arranged by size: 1911 1280 1079 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2137 2137 Size: 5992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2647 3090 4055 4652 5439 5707 Size: 393 612 403 443 965 597 787 268 Cuts at: 5707 1239 Size: 1524 Fragments arranged by size: 1524 965 787 612 597 443 403 393 268 EcoO109I rG'GnC_Cy Cuts at: 2234 2271 2376 2377 2733 2753 2754 2845 2846 Size: 37 105 1 356 20 1 91 1 Cuts at: 2846 2234 Size: 5380 Fragments arranged by size: 5380 356 105 91 37 20 1 1 1 EcoRI G'AATT_C Cuts at: 2922 3301 3679 2922 Size: 379 378 5235 Fragments arranged by size: 5235 379 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2060 2060 Size: 5992 FspI TGC'GCA Cuts at: 4498 4498 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 5139 1678 Size: 3461 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 2643 2675 3104 Size: 32 135 32 890 32 149 32 429 Cuts at: 3104 3136 3242 3274 4633 4665 4707 4739 1373 Size: 32 106 32 1359 32 42 32 2626 Fragments arranged by size: 2626 1359 890 429 149 135 106 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3336 3582 669 Size: 988 281 1398 246 3079 Fragments arranged by size: 3079 1398 988 281 246 HindIII A'AGCT_T Cuts at: 748 2904 748 Size: 2156 3836 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5106 5290 1305 Size: 3801 184 2007 Fragments arranged by size: 3801 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4170 Fragments arranged by size: 4170 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2768 2811 3950 4309 4468 518 Size: 648 1602 43 1139 359 159 2042 Fragments arranged by size: 2042 1602 1139 648 359 159 43 MspA1I CmG'CkG Cuts at: 1606 2104 2592 2800 3408 4085 5233 5478 1606 Size: 498 488 208 608 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 608 498 488 245 208 NaeI GCC'GGC Cuts at: 2896 2896 Size: 5992 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4501 Fragments arranged by size: 4501 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NgoMIV G'CCGG_C Cuts at: 2894 2894 Size: 5992 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2285 3350 4895 1132 Size: 97 1056 1065 1545 2229 Fragments arranged by size: 2229 1545 1065 1056 97 PasI CC'CwG_GG Cuts at: 1348 2748 3035 1348 Size: 1400 287 4305 Fragments arranged by size: 4305 1400 287 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 2487 2592 3247 1108 Size: 1290 89 105 655 3853 Fragments arranged by size: 3853 1290 655 105 89 PfoI T'CCnGG_A Cuts at: 2932 2932 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4061 4093 5574 5606 4061 Size: 32 1481 32 4447 Fragments arranged by size: 4447 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2234 2733 2234 Size: 499 5493 PshAI GACnn'nnGTC Cuts at: 1613 2364 3096 3147 1613 Size: 751 732 51 4458 Fragments arranged by size: 4458 751 732 51 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PspOMI G'GGCC_C Cuts at: 2376 2753 2845 2376 Size: 377 92 5523 Fragments arranged by size: 5523 377 92 PstI C_TGCA'G Cuts at: 830 2064 2242 3232 3344 830 Size: 1234 178 990 112 3478 Fragments arranged by size: 3478 1234 990 178 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 PvuII CAG'CTG Cuts at: 2104 2592 2800 2104 Size: 488 208 5296 Fragments arranged by size: 5296 488 208 SacI G_AGCT'C Cuts at: 2139 2139 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SanDI GG'GwC_CC Cuts at: 2733 2733 Size: 5992 SapI GCTCTTCn'nnn_ Cuts at: 2441 2515 2441 Size: 74 5918 SbfI CC_TGCA'GG Cuts at: 2064 3344 2064 Size: 1280 4712 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SexAI A'CCwGG_T Cuts at: 2198 2710 2198 Size: 512 5480 SfcI C'TryA_G Cuts at: 826 1045 2060 2238 2344 3228 3340 4475 5156 Size: 219 1015 178 106 884 112 1135 681 Cuts at: 5156 5347 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1015 884 681 219 191 178 112 106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 2028 3319 1872 Size: 156 1291 4545 Fragments arranged by size: 4545 1291 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 2990 3138 4096 4997 5259 820 Size: 197 926 1047 148 958 901 262 1553 Fragments arranged by size: 1553 1047 958 926 901 262 197 148 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 3350 1132 Size: 2218 3774 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5992 StyI C'CwwG_G Cuts at: 513 1065 2004 3431 513 Size: 552 939 1427 3074 Fragments arranged by size: 3074 1427 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2140 4019 4036 4189 4374 4819 325 Size: 1255 560 1879 17 153 185 445 1498 Fragments arranged by size: 1879 1498 1255 560 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2436 4238 5950 Size: 274 80 33 51 1443 459 1802 1712 Cuts at: 5950 96 Size: 138 Fragments arranged by size: 1802 1712 1443 459 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2679 2711 5952 5984 2679 Size: 32 3241 32 2687 Fragments arranged by size: 3241 2687 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5992 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2861 2203 Size: 658 5334 XhoI C'TCGA_G Cuts at: 1943 2990 3138 1943 Size: 1047 148 4797 Fragments arranged by size: 4797 1047 148 XmaI C'CCGG_G Cuts at: 1870 2026 3317 1870 Size: 156 1291 4545 Fragments arranged by size: 4545 1291 156 XmnI GAAnn'nnTTC Cuts at: 4121 4121 Size: 5992 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI