(Circular) (Six-base) MAPSORT of: pFN21AB4189.seq Check: 2596 from: 1 to: 6970 pFN21AB4189 6970 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4780 278 Size: 53 83 186 936 3244 2468 Fragments arranged by size: 3244 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2409 4313 1937 Size: 472 1904 4594 Fragments arranged by size: 4594 1904 472 Acc65I G'GTAC_C Cuts at: 4291 4291 Size: 6970 AclI AA'CG_TT Cuts at: 1563 5097 5470 1563 Size: 3534 373 3063 Fragments arranged by size: 3534 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2217 2684 3399 3426 3480 3507 3588 3612 Size: 585 467 715 27 54 27 81 24 Cuts at: 3612 3696 5033 6417 6685 1632 Size: 84 1337 1384 268 1917 Fragments arranged by size: 1917 1384 1337 715 585 467 268 84 81 54 27 27 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6773 AflIII A'CryG_T Cuts at: 1203 2412 2638 5869 6846 1203 Size: 1209 226 3231 977 1327 Fragments arranged by size: 3231 1327 1209 977 226 AgeI A'CCGG_T Cuts at: 5801 5801 Size: 6970 AhdI GACnn_n'nnGTC Cuts at: 5699 5699 Size: 6970 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6970 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6970 AlwNI CAG_nnn'CTG Cuts at: 3164 4713 6285 3164 Size: 1549 1572 3849 Fragments arranged by size: 3849 1572 1549 ApaI G_GGCC'C Cuts at: 4215 4215 Size: 6970 ApaLI G'TGCA_C Cuts at: 5027 6183 5027 Size: 1156 5814 ApoI r'AATT_y Cuts at: 1493 2447 2504 3320 3695 3806 4279 4505 4657 Size: 954 57 816 375 111 473 226 152 Cuts at: 4657 6766 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 954 816 473 375 226 152 111 57 AseI AT'TA_AT Cuts at: 160 1032 5524 160 Size: 872 4492 1606 Fragments arranged by size: 4492 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 AvaI C'yCGr_G Cuts at: 1870 1943 2126 3713 4295 1870 Size: 73 183 1587 582 4545 Fragments arranged by size: 4545 1587 582 183 73 BamHI G'GATC_C Cuts at: 2965 4300 4667 2965 Size: 1335 367 5268 Fragments arranged by size: 5268 1335 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2931 4291 5746 618 Size: 325 217 627 225 919 1360 1455 1842 Fragments arranged by size: 1842 1455 1360 919 627 325 225 217 BanII G_rGCy'C Cuts at: 1391 4215 4289 1391 Size: 2824 74 4072 Fragments arranged by size: 4072 2824 74 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2397 2877 3527 3660 928 Size: 971 498 480 650 133 4238 Fragments arranged by size: 4238 971 650 498 480 133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5161 5195 1903 Size: 34 3224 34 3678 Fragments arranged by size: 3678 3224 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3892 4862 6072 3892 Size: 970 1210 4790 Fragments arranged by size: 4790 1210 970 BclI T'GATC_A Cuts at: 1548 1548 Size: 6970 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5581 136 Size: 107 122 71 631 605 3909 1525 Fragments arranged by size: 3909 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6965 6965 Size: 6970 BlpI GC'TnA_GC Cuts at: 4387 4387 Size: 6970 Bme1580I G_kGCm'C Cuts at: 1790 4215 5031 6187 1790 Size: 2425 816 1156 2573 Fragments arranged by size: 2573 2425 1156 816 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6970 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4701 5659 442 Size: 416 53 83 399 24 3284 958 1753 Fragments arranged by size: 3284 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6970 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2508 3094 3865 2508 Size: 586 771 5613 Fragments arranged by size: 5613 771 586 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2412 5630 1239 Size: 1173 3218 2579 Fragments arranged by size: 3218 2579 1173 Bpu10I CC'TnA_GC Cuts at: 3351 3351 Size: 6970 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3064 3228 5095 5960 6258 3064 Size: 164 1867 865 298 3776 Fragments arranged by size: 3776 1867 865 298 164 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2746 3716 5633 882 Size: 617 1247 970 1917 2219 Fragments arranged by size: 2219 1917 1247 970 617 BsaAI yAC'GTr Cuts at: 493 1204 2413 4761 493 Size: 711 1209 2348 2702 Fragments arranged by size: 2702 2348 1209 711 BsaBI GATnn'nnATC Cuts at: 6964 6964 Size: 6970 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4777 5159 5794 275 Size: 53 83 186 936 3244 382 635 1451 Fragments arranged by size: 3244 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5403 5801 6075 6222 6633 5403 Size: 398 274 147 411 5740 Fragments arranged by size: 5740 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2131 2161 1178 Size: 30 165 30 728 30 5987 Fragments arranged by size: 5987 728 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3611 3908 4145 1186 Size: 2425 297 237 4011 Fragments arranged by size: 4011 2425 297 237 BseYI C'CCAG_C Cuts at: 3221 6173 6669 3221 Size: 2952 496 3522 Fragments arranged by size: 3522 2952 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6397 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4420 5181 6209 1540 Size: 275 186 2419 761 1028 2301 Fragments arranged by size: 2419 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3219 5031 5116 6187 3219 Size: 1812 85 1071 4002 Fragments arranged by size: 4002 1812 1071 85 BsmI GAATG_Cn' Cuts at: 2061 3244 4481 4574 2061 Size: 1183 1237 93 4457 Fragments arranged by size: 4457 1237 1183 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2067 2544 2067 Size: 477 6493 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3219 4215 4289 5031 5116 6187 1391 Size: 399 1429 996 74 742 85 1071 2174 Fragments arranged by size: 2174 1429 1071 996 742 399 85 74 BspEI T'CCGG_A Cuts at: 6633 6633 Size: 6970 BspHI T'CATG_A Cuts at: 3754 4859 6589 3754 Size: 1105 1730 4135 Fragments arranged by size: 4135 1730 1105 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4325 844 Size: 3481 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2010 4857 1394 Size: 429 187 2847 3507 Fragments arranged by size: 3507 2847 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 2205 5465 5639 65 Size: 1183 957 3260 174 1396 Fragments arranged by size: 3260 1396 1183 957 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2105 5614 5801 1700 Size: 110 295 3509 187 2869 Fragments arranged by size: 3509 2869 295 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6970 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6970 BssSI C'ACGA_G Cuts at: 5030 6042 5030 Size: 1012 5958 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6970 BstBI TT'CG_AA Cuts at: 2891 4095 5774 2891 Size: 1204 1679 4087 Fragments arranged by size: 4087 1679 1204 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6970 BstYI r'GATC_y Cuts at: 1972 2488 2965 3482 4300 4667 5052 5069 6521 Size: 516 477 517 818 367 385 17 1452 Cuts at: 6521 6607 6619 6965 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 818 517 516 477 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2410 2410 Size: 6970 Bsu36I CC'TnA_GG Cuts at: 3132 3149 3132 Size: 17 6953 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6418 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4263 487 Size: 683 56 3037 3194 Fragments arranged by size: 3194 3037 683 56 BtsI GCAGTG_nn' Cuts at: 3030 4486 5280 5300 3030 Size: 1456 794 20 4700 Fragments arranged by size: 4700 1456 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6970 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6935 DraI TTT'AAA Cuts at: 4274 4274 Size: 6970 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5977 809 Size: 507 4661 1802 Fragments arranged by size: 4661 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3019 4417 5306 Size: 54 1425 50 275 18 1189 1398 889 Cuts at: 5306 6935 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1398 1189 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4417 1537 Size: 275 2605 4090 Fragments arranged by size: 4090 2605 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3402 3483 3537 3588 3885 4020 4900 1353 Size: 2049 81 54 51 297 135 880 3423 Fragments arranged by size: 3423 2049 880 297 135 81 54 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2124 4007 5557 5931 6077 387 Size: 1064 138 535 1883 1550 374 146 1280 Fragments arranged by size: 1883 1550 1280 1064 535 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 2412 2684 3399 3426 3480 3507 Size: 393 585 195 272 715 27 54 27 Cuts at: 3507 3588 3612 3696 5033 5630 6417 6685 1239 Size: 81 24 84 1337 597 787 268 1524 Fragments arranged by size: 1524 1337 787 715 597 585 393 272 268 195 84 81 54 27 27 24 EcoNI CCTnn'n_nnAGG Cuts at: 3557 3581 3557 Size: 24 6946 EcoO109I rG'GnC_Cy Cuts at: 2161 3188 4211 4212 2161 Size: 1027 1023 1 4919 Fragments arranged by size: 4919 1027 1023 1 EcoRI G'AATT_C Cuts at: 4279 4657 4279 Size: 378 6592 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2558 2558 Size: 6970 FspI TGC'GCA Cuts at: 5476 5476 Size: 6970 HaeII r_GCGC'y Cuts at: 1678 6117 1678 Size: 4439 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2131 2163 2234 2266 2787 Size: 32 135 32 559 32 71 32 521 Cuts at: 2787 2819 3152 3184 3629 3661 3806 3838 3911 Size: 32 333 32 445 32 145 32 73 Cuts at: 3911 3943 5611 5643 5685 5717 1373 Size: 32 1668 32 42 32 2626 Fragments arranged by size: 2626 1668 559 521 445 333 145 135 73 71 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4314 4560 669 Size: 988 281 2376 246 3079 Fragments arranged by size: 3079 2376 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6970 HpaI GTT'AAC Cuts at: 4560 4560 Size: 6970 KpnI G_GTAC'C Cuts at: 4295 4295 Size: 6970 MfeI C'AATT_G Cuts at: 4569 4569 Size: 6970 MluI A'CGCG_T Cuts at: 6846 6846 Size: 6970 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2724 6084 6268 1305 Size: 1419 3360 184 2007 Fragments arranged by size: 3360 2007 1419 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5148 Fragments arranged by size: 5148 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 2937 3759 4928 5287 5446 518 Size: 648 1314 457 822 1169 359 159 2042 Fragments arranged by size: 2042 1314 1169 822 648 457 359 159 MspA1I CmG'CkG Cuts at: 1606 3441 4386 5063 6211 6456 1606 Size: 1835 945 677 1148 245 2120 Fragments arranged by size: 2120 1835 1148 945 677 245 NaeI GCC'GGC Cuts at: 2107 2107 Size: 6970 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6418 NdeI CA'TA_TG Cuts at: 387 3904 387 Size: 3517 3453 NgoMIV G'CCGG_C Cuts at: 2105 2105 Size: 6970 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6970 NotI GC'GGCC_GC Cuts at: 4417 4417 Size: 6970 NspI r_CATG'y Cuts at: 1132 1229 2642 4328 5873 1132 Size: 97 1413 1686 1545 2229 Fragments arranged by size: 2229 1686 1545 1413 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6970 PciI A'CATG_T Cuts at: 2638 5869 2638 Size: 3231 3739 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6970 PfoI T'CCnGG_A Cuts at: 2777 2777 Size: 6970 PmeI GTTT'AAAC Cuts at: 4274 4274 Size: 6970 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5039 5071 6552 6584 5039 Size: 32 1481 32 5425 Fragments arranged by size: 5425 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2161 2161 Size: 6970 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6970 PsiI TTA'TAA Cuts at: 4540 4540 Size: 6970 PspOMI G'GGCC_C Cuts at: 4211 4211 Size: 6970 PstI C_TGCA'G Cuts at: 830 2296 4322 830 Size: 1466 2026 3478 Fragments arranged by size: 3478 2026 1466 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6970 PvuII CAG'CTG Cuts at: 3441 3441 Size: 6970 SalI G'TCGA_C Cuts at: 1936 4312 1936 Size: 2376 4594 SbfI CC_TGCA'GG Cuts at: 4322 4322 Size: 6970 ScaI AGT'ACT Cuts at: 5218 6930 5218 Size: 1712 5258 SfcI C'TryA_G Cuts at: 826 1045 2292 3054 3279 4318 5453 6134 6325 Size: 219 1247 762 225 1039 1135 681 191 Cuts at: 6325 826 Size: 1471 Fragments arranged by size: 1471 1247 1135 1039 762 681 225 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6970 SmaI CCC'GGG Cuts at: 1872 4297 1872 Size: 2425 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3079 3243 3713 5074 5975 6237 Size: 197 926 1136 164 470 1361 901 262 Cuts at: 6237 820 Size: 1553 Fragments arranged by size: 1553 1361 1136 926 901 470 262 197 164 SnaBI TAC'GTA Cuts at: 493 4761 493 Size: 4268 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6970 SphI G_CATG'C Cuts at: 1132 4328 1132 Size: 3196 3774 SspI AAT'ATT Cuts at: 5 52 4894 5 Size: 47 4842 2081 Fragments arranged by size: 4842 2081 47 StuI AGG'CCT Cuts at: 1517 3147 1517 Size: 1630 5340 StyI C'CwwG_G Cuts at: 513 1065 4409 513 Size: 552 3344 3074 Fragments arranged by size: 3344 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2108 2114 2949 4175 4997 5014 5167 Size: 1255 528 6 835 1226 822 17 153 Cuts at: 5167 5352 5797 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1226 835 822 528 445 185 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5216 6928 96 Size: 274 80 33 51 1443 3239 1712 138 Fragments arranged by size: 3239 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4171 4203 6930 6962 4171 Size: 32 2727 32 4179 Fragments arranged by size: 4179 2727 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6970 XbaI T'CTAG_A Cuts at: 3194 4306 3194 Size: 1112 5858 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3044 3044 Size: 6970 XhoI C'TCGA_G Cuts at: 1943 3713 1943 Size: 1770 5200 XmaI C'CCGG_G Cuts at: 1870 4295 1870 Size: 2425 4545 XmnI GAAnn'nnTTC Cuts at: 2874 3762 5099 2874 Size: 888 1337 4745 Fragments arranged by size: 4745 1337 888 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4778 276 Size: 53 83 186 936 3244 2468 Fragments arranged by size: 3244 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI