(Linear) (Six-base) MAPSORT of: KIBB4189.seq Check: 8187 from: 1 to: 2265 KIBB4189.orf With 182 enzymes: SgfI * December 5, 2014 12:53 .. AccI GT'mk_AC Cuts at: 0 404 2265 Size: 404 1861 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 679 1394 1421 1475 1502 1583 1607 Size: 212 467 715 27 54 27 81 24 Cuts at: 1607 1691 2265 Size: 84 574 Fragments arranged by size: 715 574 467 212 84 81 54 27 27 24 AflIII A'CryG_T Cuts at: 0 407 633 2265 Size: 407 226 1632 Fragments arranged by size: 1632 407 226 AlwNI CAG_nnn'CTG Cuts at: 0 1159 2265 Size: 1159 1106 ApaI G_GGCC'C Cuts at: 0 2210 2265 Size: 2210 55 ApoI r'AATT_y Cuts at: 0 442 499 1315 1690 1801 2265 Size: 442 57 816 375 111 464 Fragments arranged by size: 816 464 442 375 111 57 AvaI C'yCGr_G Cuts at: 0 121 1708 2265 Size: 121 1587 557 Fragments arranged by size: 1587 557 121 BamHI G'GATC_C Cuts at: 0 960 2265 Size: 960 1305 BanI G'GyrC_C Cuts at: 0 7 926 2265 Size: 7 919 1339 Fragments arranged by size: 1339 919 7 BanII G_rGCy'C Cuts at: 0 2210 2265 Size: 2210 55 BbsI GAAGACnn'nnnn_ Cuts at: 0 392 872 1522 1655 2265 Size: 392 480 650 133 610 Fragments arranged by size: 650 610 480 392 133 BciVI GTATCCnnnnn_n' Cuts at: 0 1887 2265 Size: 1887 378 Bme1580I G_kGCm'C Cuts at: 0 2210 2265 Size: 2210 55 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 503 1089 1860 2265 Size: 503 586 771 405 Fragments arranged by size: 771 586 503 405 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 407 2265 Size: 407 1858 Bpu10I CC'TnA_GC Cuts at: 0 1346 2265 Size: 1346 919 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1059 1223 2265 Size: 1059 164 1042 Fragments arranged by size: 1059 1042 164 BsaI GGTCTCn'nnnn_ Cuts at: 0 741 1711 2265 Size: 741 970 554 Fragments arranged by size: 970 741 554 BsaAI yAC'GTr Cuts at: 0 408 2265 Size: 408 1857 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 126 156 2265 Size: 126 30 2109 Fragments arranged by size: 2109 126 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1606 1903 2140 2265 Size: 1606 297 237 125 Fragments arranged by size: 1606 297 237 125 BseYI C'CCAG_C Cuts at: 0 1216 2265 Size: 1216 1049 BsiHKAI G_wGCw'C Cuts at: 0 1214 2265 Size: 1214 1051 BsmI GAATG_Cn' Cuts at: 0 56 1239 2265 Size: 56 1183 1026 Fragments arranged by size: 1183 1026 56 BsmBI CGTCTCn'nnnn_ Cuts at: 0 62 539 2265 Size: 62 477 1726 Fragments arranged by size: 1726 477 62 Bsp1286I G_dGCh'C Cuts at: 0 1214 2210 2265 Size: 1214 996 55 Fragments arranged by size: 1214 996 55 BspHI T'CATG_A Cuts at: 0 1749 2265 Size: 1749 516 BsrBI CCG'CTC Cuts at: 0 5 2265 Size: 5 2260 BsrDI GCAATG_nn' Cuts at: 0 200 2265 Size: 200 2065 BsrFI r'CCGG_y Cuts at: 0 100 2265 Size: 100 2165 BstBI TT'CG_AA Cuts at: 0 886 2090 2265 Size: 886 1204 175 Fragments arranged by size: 1204 886 175 BstYI r'GATC_y Cuts at: 0 483 960 1477 2265 Size: 483 477 517 788 Fragments arranged by size: 788 517 483 477 BstZ17I GTA'TAC Cuts at: 0 405 2265 Size: 405 1860 Bsu36I CC'TnA_GG Cuts at: 0 1127 1144 2265 Size: 1127 17 1121 Fragments arranged by size: 1127 1121 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2258 2265 Size: 2258 7 BtsI GCAGTG_nn' Cuts at: 0 1025 2265 Size: 1025 1240 EaeI y'GGCC_r Cuts at: 0 1014 2265 Size: 1014 1251 EarI CTCTTCn'nnn_ Cuts at: 0 1397 1478 1532 1583 1880 2015 2265 Size: 1397 81 54 51 297 135 250 Fragments arranged by size: 1397 297 250 135 81 54 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 119 2002 2265 Size: 119 1883 263 Fragments arranged by size: 1883 263 119 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 407 679 1394 1421 1475 1502 1583 Size: 212 195 272 715 27 54 27 81 Cuts at: 1583 1607 1691 2265 Size: 24 84 574 Fragments arranged by size: 715 574 272 212 195 84 81 54 27 27 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 1552 1576 2265 Size: 1552 24 689 Fragments arranged by size: 1552 689 24 EcoO109I rG'GnC_Cy Cuts at: 0 156 1183 2206 2207 2265 Size: 156 1027 1023 1 58 Fragments arranged by size: 1027 1023 156 58 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 553 2265 Size: 553 1712 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 126 158 229 261 782 814 1147 1179 Size: 126 32 71 32 521 32 333 32 Cuts at: 1179 1624 1656 1801 1833 1906 1938 2265 Size: 445 32 145 32 73 32 327 Fragments arranged by size: 521 445 333 327 145 126 73 71 32 32 32 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 719 2265 Size: 719 1546 MslI CAynn'nnrTG Cuts at: 0 475 932 1754 2265 Size: 475 457 822 511 Fragments arranged by size: 822 511 475 457 MspA1I CmG'CkG Cuts at: 0 1436 2265 Size: 1436 829 NaeI GCC'GGC Cuts at: 0 102 2265 Size: 102 2163 NdeI CA'TA_TG Cuts at: 0 1899 2265 Size: 1899 366 NgoMIV G'CCGG_C Cuts at: 0 100 2265 Size: 100 2165 NspI r_CATG'y Cuts at: 0 637 2265 Size: 637 1628 PciI A'CATG_T Cuts at: 0 633 2265 Size: 633 1632 PfoI T'CCnGG_A Cuts at: 0 772 2265 Size: 772 1493 PpuMI rG'GwC_Cy Cuts at: 0 156 2265 Size: 156 2109 PspOMI G'GGCC_C Cuts at: 0 2206 2265 Size: 2206 59 PstI C_TGCA'G Cuts at: 0 291 2265 Size: 291 1974 PvuII CAG'CTG Cuts at: 0 1436 2265 Size: 1436 829 SfcI C'TryA_G Cuts at: 0 287 1049 1274 2265 Size: 287 762 225 991 Fragments arranged by size: 991 762 287 225 SmlI C'TyrA_G Cuts at: 0 1074 1238 1708 2265 Size: 1074 164 470 557 Fragments arranged by size: 1074 557 470 164 StuI AGG'CCT Cuts at: 0 1142 2265 Size: 1142 1123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 103 109 944 2170 2265 Size: 103 6 835 1226 95 Fragments arranged by size: 1226 835 103 95 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2166 2198 2265 Size: 2166 32 67 Fragments arranged by size: 2166 67 32 XbaI T'CTAG_A Cuts at: 0 1189 2265 Size: 1189 1076 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1039 2265 Size: 1039 1226 XhoI C'TCGA_G Cuts at: 0 1708 2265 Size: 1708 557 XmnI GAAnn'nnTTC Cuts at: 0 869 1757 2265 Size: 869 888 508 Fragments arranged by size: 888 869 508 Enzymes that do cut: AccI AcuI AflIII AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BciVI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI Hin4I MmeI MslI MspA1I NaeI NdeI NgoMIV NspI PciI PfoI PpuMI PspOMI PstI PvuII SfcI SmlI StuI TaqII TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BspEI BspMI BsrGI BssHII BssSI BstAPI BstEII BstXI BtgI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI Tth111I XmaI ZraI