################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BseRI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 849 CGCG CG^CG AcsI 23 GAATTC [GA]^AATT[CT] AfaI 297 GTAC GT^AC AlwNI 208 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 23 GAATTC [GA]^AATT[CT] BalI 191 TGGCCA TGG^CCA BbsI 610 GAAGAC GAAGAC(2/6) BbvCI 348 CCTCAGC CCTCAGC(-5/-2) BclI 555 TGATCA T^GATCA BfaI 412 CTAG C^TAG BlpI 489 GCTGAGC GC^T[ACGT]AGC Bpu1102I 489 GCTGAGC GC^T[ACGT]AGC BpuAI 610 GAAGAC GAAGAC(2/6) BsiEI 1018 CGGCCG CG[GA][CT]^CG BsmI 401 GAATGC GAATGC(1/-1) BspHI 173 TCATGA T^CATGA BsrBI 98 GAGCGG CCGCTC(-3/-3) BsrFI 503 GCCGGC [GA]^CCGG[CT] BstAPI 510 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 849 CGCG CG^CG CelII 489 GCTGAGC GC^T[ACGT]AGC Cfr10I 503 GCCGGC [GA]^CCGG[CT] DsaI 119 CCACGG C^C[GA][CT]GG EagI 1018 CGGCCG C^GGCCG EclXI 1018 CGGCCG C^GGCCG Eco52I 1018 CGGCCG C^GGCCG EcoRI 23 GAATTC G^AATTC EcoT14I 392 CCAAGG C^C[AT][AT]GG FbaI 555 TGATCA T^GATCA HgaI 847 GACGC GACGC(5/10) MaeI 412 CTAG C^TAG MluNI 191 TGGCCA TGG^CCA MscI 191 TGGCCA TGG^CCA MvnI 849 CGCG CG^CG NaeI 503 GCCGGC GCC^GGC NgoMIV 503 GCCGGC G^CCGGC PstI 214 CTGCAG CTGCA^G RcaI 173 TCATGA T^CATGA RsaI 297 GTAC GT^AC SfcI 214 CTGCAG C^T[GA][CT]AG SgrAI 502 CGCCGGCG C[GA]^CCGG[CT]G SmlI 667 CTCAAG C^T[CT][GA]AG SphI 1083 GCATGC GCATG^C StyI 392 CCAAGG C^C[AT][AT]GG Tsp509I 24 AATT ^AATT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccIII 743 TCCGGA T^CCGGA 971 TCCGGA AocI 577 CCTGAGG CC^T[ACGT]AGG 772 CCTGAGG AvaI 632 CCCGGG C^[CT]CG[GA]G 1121 CCCGGG BsaI 640 GAGACC GGTCTC(1/5) 1069 GAGACC BseAI 743 TCCGGA T^CCGGA 971 TCCGGA BsoBI 632 CCCGGG C^[CT]CG[GA]G 1121 CCCGGG BspEI 743 TCCGGA T^CCGGA 971 TCCGGA BspMI 263 ACCTGC ACCTGC(4/8) 368 ACCTGC BsrI 680 CCAGT ACTGG(1/-1) 929 CCAGT Bsu36I 577 CCTGAGG CC^T[ACGT]AGG 772 CCTGAGG DraIII 109 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 1001 CACAGGGTG EaeI 191 TGGCCA [CT]^GGCC[GA] 1018 CGGCCG EarI 751 CTCTTC CTCTTC(1/4) 877 GAAGAG Eco81I 577 CCTGAGG CC^T[ACGT]AGG 772 CCTGAGG FauI 500 CCCGC CCCGC(4/6) 327 GCGGG Ksp632I 751 CTCTTC CTCTTC(1/4) 877 GAAGAG MroI 743 TCCGGA T^CCGGA 971 TCCGGA NspI 515 ACATGC [GA]CATG^[CT] 1083 GCATGC PpuMI 365 GGGACCT [GA]G^G[AT]CC[CT] 564 GGGTCCC SfaNI 236 GCATC GCATC(5/9) 734 GCATC SmaI 632 CCCGGG CCC^GGG 1121 CCCGGG XcmI 431 CCATGATTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1101 CCACTCCCGTCATGG XmaCI 632 CCCGGG C^CCGGG 1121 CCCGGG XmaI 632 CCCGGG C^CCGGG 1121 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AluI ApaI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII SacI Sau3AI Sau96I ScrFI TaqI TfiI TseI Tsp45I TspRI TthHB8I