################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FspI HincII HindII HindIII HinfI HpaI KpnI Ksp632I KspI MaeI MaeII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AlwNI 165 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 160 GGGCCC GGGCC^C BanI 453 GGCGCC G^G[CT][GA]CC BbeI 453 GGCGCC GGCGC^C BbvCI 208 GCTGAGG CCTCAGC(-5/-2) BpmI 106 CTGGAG CTGGAG(16/14) BsaWI 383 ACCGGA [AT]^CCGG[AT] BsgI 439 GTGCAG GTGCAG(16/14) BsmAI 114 GAGAC GTCTC(1/5) BspMI 239 ACCTGC ACCTGC(4/8) BsrBI 279 GAGCGG CCGCTC(-3/-3) BsrFI 411 GCCGGC [GA]^CCGG[CT] BstAPI 167 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 395 CCACGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 411 GCCGGC [GA]^CCGG[CT] DraII 418 GGGGCCT [GA]G^G[ACGT]CC[CT] EagI 413 CGGCCG C^GGCCG EclXI 413 CGGCCG C^GGCCG Eco52I 413 CGGCCG C^GGCCG EcoO109I 418 GGGGCCT [GA]G^G[ACGT]CC[CT] FseI 410 GGCCGGCC GGCCGG^CC HgaI 484 GACGC GACGC(5/10) HphI 360 TCACC GGTGA(8/7) KasI 453 GGCGCC G^GCGCC MaeIII 331 GTGAC ^GT[ACGT]AC NaeI 411 GCCGGC GCC^GGC NarI 453 GGCGCC GG^CGCC NgoMIV 411 GCCGGC G^CCGGC SfaNI 167 GCATC GCATC(5/9) SfoI 453 GGCGCC GGC^GCC SmaI 136 CCCGGG CCC^GGG TaqI 467 TCGA T^CGA Tsp45I 331 GTGAC ^GT[GC]AC TspRI 118 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 467 TCGA T^CGA XcmI 95 CCATGTCCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 136 CCCGGG C^CCGGG XmaI 136 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcyI 453 GGCGCC G[GA]^CG[CT]C 484 GACGCC BsaHI 453 GGCGCC G[GA]^CG[CT]C 484 GACGCC BseRI 48 CTCCTC GAGGAG(10/8) 229 GAGGAG BsiEI 381 CGACCG CG[GA][CT]^CG 413 CGGCCG BsiYI 76 CCGAGGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 377 CCAGCGACCGG BslI 76 CCGAGGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 377 CCAGCGACCGG BsrI 39 ACTGG ACTGG(1/-1) 255 CCAGT BstNI 105 CCTGG CC^[AT]GG 225 CCAGG DdeI 112 CTGAG C^T[ACGT]AG 209 CTGAG EcoRII 105 CCTGG ^CC[AT]GG 225 CCAGG HaeII 213 GGCGCT [GA]GCGC^[CT] 453 GGCGCC Hin1I 453 GGCGCC G[GA]^CG[CT]C 484 GACGCC MvaI 105 CCTGG CC^[AT]GG 225 CCAGG NlaIII 12 CATG CATG^ 96 CATG PvuII 400 CAGCTG CAG^CTG 442 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AluI AvaI BanII BbvI BcnI BglI BmyI BsaJI BsoBI Bsp1286I BssKI BstUI Cac8I CfoI Cfr13I EaeI FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI