(Circular) (Six-base) MAPSORT of: pFN21AE1648.seq Check: 6997 from: 1 to: 5197 pFN21AE1648 5197 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3007 278 Size: 53 83 186 936 1471 2468 Fragments arranged by size: 2468 1471 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2540 1937 Size: 603 4594 Acc65I G'GTAC_C Cuts at: 2518 2518 Size: 5197 AclI AA'CG_TT Cuts at: 1563 3324 3697 1563 Size: 1761 373 3063 Fragments arranged by size: 3063 1761 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3260 4644 4912 1632 Size: 1628 1384 268 1917 Fragments arranged by size: 1917 1628 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5000 AflIII A'CryG_T Cuts at: 1203 4096 5073 1203 Size: 2893 977 1327 Fragments arranged by size: 2893 1327 977 AgeI A'CCGG_T Cuts at: 4028 4028 Size: 5197 AhdI GACnn_n'nnGTC Cuts at: 3926 3926 Size: 5197 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5197 AlwNI CAG_nnn'CTG Cuts at: 2175 2940 4512 2175 Size: 765 1572 2860 Fragments arranged by size: 2860 1572 765 ApaI G_GGCC'C Cuts at: 2169 2169 Size: 5197 ApaLI G'TGCA_C Cuts at: 3254 4410 3254 Size: 1156 4041 ApoI r'AATT_y Cuts at: 1493 2506 2732 2884 4993 1493 Size: 1013 226 152 2109 1697 Fragments arranged by size: 2109 1697 1013 226 152 AseI AT'TA_AT Cuts at: 160 1032 3751 160 Size: 872 2719 1606 Fragments arranged by size: 2719 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5197 AvaI C'yCGr_G Cuts at: 1870 1943 2080 2141 2345 2462 2522 1870 Size: 73 137 61 204 117 60 4545 Fragments arranged by size: 4545 204 137 117 73 61 60 BamHI G'GATC_C Cuts at: 2527 2894 2527 Size: 367 4830 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2458 2518 3973 618 Size: 325 217 627 671 60 1455 1842 Fragments arranged by size: 1842 1455 671 627 325 217 60 BanII G_rGCy'C Cuts at: 1391 2169 2346 2469 2478 2516 1391 Size: 778 177 123 9 38 4072 Fragments arranged by size: 4072 778 177 123 38 9 BbeI G_GCGC'C Cuts at: 2462 2462 Size: 5197 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4226 BbvCI CC'TCA_GC Cuts at: 2214 2214 Size: 5197 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3388 3422 1903 Size: 34 50 34 1367 34 3678 Fragments arranged by size: 3678 1367 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3089 4299 3089 Size: 1210 3987 BclI T'GATC_A Cuts at: 1548 1548 Size: 5197 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2085 2145 2265 Size: 107 122 71 631 605 413 60 120 Cuts at: 2265 2358 3808 136 Size: 93 1450 1525 Fragments arranged by size: 1525 1450 631 605 413 122 120 107 93 71 60 BglII A'GATC_T Cuts at: 5192 5192 Size: 5197 BlpI GC'TnA_GC Cuts at: 2614 2614 Size: 5197 Bme1580I G_kGCm'C Cuts at: 1790 2169 2210 3258 4414 1790 Size: 379 41 1048 1156 2573 Fragments arranged by size: 2573 1156 1048 379 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5197 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2928 3886 442 Size: 416 53 83 399 24 1511 958 1753 Fragments arranged by size: 1753 1511 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5197 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 3857 1239 Size: 893 1725 2579 Fragments arranged by size: 2579 1725 893 Bpu10I CC'TnA_GC Cuts at: 2214 2214 Size: 5197 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3322 4187 4485 3322 Size: 865 298 4034 Fragments arranged by size: 4034 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3860 882 Size: 617 2361 2219 Fragments arranged by size: 2361 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2988 493 Size: 711 1784 2702 Fragments arranged by size: 2702 1784 711 BsaBI GATnn'nnATC Cuts at: 5191 5191 Size: 5197 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2459 2490 3004 3386 Size: 53 83 186 936 926 31 514 382 Cuts at: 3386 4021 275 Size: 635 1451 Fragments arranged by size: 1451 936 926 635 514 382 186 83 53 31 BsaWI w'CCGG_w Cuts at: 2388 3630 4028 4302 4449 4860 2388 Size: 1242 398 274 147 411 2725 Fragments arranged by size: 2725 1242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2112 2142 1178 Size: 30 165 30 709 30 4233 Fragments arranged by size: 4233 709 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2249 1186 Size: 858 205 4134 Fragments arranged by size: 4134 858 205 BseYI C'CCAG_C Cuts at: 2168 2191 2481 4400 4896 2168 Size: 23 290 1919 496 2469 Fragments arranged by size: 2469 1919 496 290 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 1163 Size: 573 729 3895 Fragments arranged by size: 3895 729 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2389 2421 2647 3408 4436 1540 Size: 275 186 388 32 226 761 1028 2301 Fragments arranged by size: 2301 1028 761 388 275 226 186 32 BsiHKAI G_wGCw'C Cuts at: 3258 3343 4414 3258 Size: 85 1071 4041 Fragments arranged by size: 4041 1071 85 BsmI GAATG_Cn' Cuts at: 2708 2801 2708 Size: 93 5104 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2210 2346 2469 2478 2516 3258 Size: 399 379 41 136 123 9 38 742 Cuts at: 3258 3343 4414 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 136 123 85 41 38 9 BspEI T'CCGG_A Cuts at: 4860 4860 Size: 5197 BspHI T'CATG_A Cuts at: 3086 4816 3086 Size: 1730 3467 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2253 2552 844 Size: 1409 299 3489 Fragments arranged by size: 3489 1409 299 BsrBI CCG'CTC Cuts at: 1394 1823 2286 3084 1394 Size: 429 463 798 3507 Fragments arranged by size: 3507 798 463 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3692 3866 65 Size: 1183 2444 174 1396 Fragments arranged by size: 2444 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2416 3841 4028 1700 Size: 110 606 1425 187 2869 Fragments arranged by size: 2869 1425 606 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5197 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5197 BssSI C'ACGA_G Cuts at: 3257 4269 3257 Size: 1012 4185 BstAPI GCAn_nnn'nTGC Cuts at: 794 2178 794 Size: 1384 3813 BstBI TT'CG_AA Cuts at: 4001 4001 Size: 5197 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5197 BstXI CCAn_nnnn'nTGG Cuts at: 2407 2407 Size: 5197 BstYI r'GATC_y Cuts at: 1972 2527 2894 3279 3296 4748 4834 4846 5192 Size: 555 367 385 17 1452 86 12 346 Cuts at: 5192 1972 Size: 1977 Fragments arranged by size: 1977 1452 555 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4645 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4458 Fragments arranged by size: 4458 683 56 BtsI GCAGTG_nn' Cuts at: 2713 3507 3527 2713 Size: 794 20 4383 Fragments arranged by size: 4383 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5197 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5162 DraI TTT'AAA Cuts at: 2501 2501 Size: 5197 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4204 809 Size: 507 2888 1802 Fragments arranged by size: 2888 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2266 2418 2451 Size: 54 1425 50 275 18 436 152 33 Cuts at: 2451 2644 3533 5162 8 Size: 193 889 1629 43 Fragments arranged by size: 1629 1425 889 436 275 193 152 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2418 2644 1537 Size: 275 606 226 4090 Fragments arranged by size: 4090 606 275 226 EarI CTCTTCn'nnn_ Cuts at: 1353 3127 1353 Size: 1774 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3784 4158 4304 387 Size: 1064 138 2195 374 146 1280 Fragments arranged by size: 2195 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 3260 3857 4644 4912 1239 Size: 393 500 1128 597 787 268 1524 Fragments arranged by size: 1524 1128 787 597 500 393 268 EcoO109I rG'GnC_Cy Cuts at: 2424 2424 Size: 5197 EcoRI G'AATT_C Cuts at: 2506 2884 2506 Size: 378 4819 FseI GG_CCGG'CC Cuts at: 2420 2420 Size: 5197 FspI TGC'GCA Cuts at: 3703 3703 Size: 5197 HaeII r_GCGC'y Cuts at: 1678 2222 2462 4344 1678 Size: 544 240 1882 2531 Fragments arranged by size: 2531 1882 544 240 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2112 2144 3838 3870 3912 Size: 32 135 32 540 32 1694 32 42 Cuts at: 3912 3944 1373 Size: 32 2626 Fragments arranged by size: 2626 1694 540 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2541 2787 669 Size: 988 281 603 246 3079 Fragments arranged by size: 3079 988 603 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5197 HpaI GTT'AAC Cuts at: 2787 2787 Size: 5197 KasI G'GCGC_C Cuts at: 2458 2458 Size: 5197 KpnI G_GTAC'C Cuts at: 2522 2522 Size: 5197 MfeI C'AATT_G Cuts at: 2796 2796 Size: 5197 MluI A'CGCG_T Cuts at: 5073 5073 Size: 5197 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 4311 4495 1305 Size: 825 2181 184 2007 Fragments arranged by size: 2181 2007 825 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3375 Fragments arranged by size: 3375 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3155 3514 3673 518 Size: 648 1989 359 159 2042 Fragments arranged by size: 2042 1989 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2157 2295 2407 2449 2613 3290 4438 4683 Size: 551 138 112 42 164 677 1148 245 Cuts at: 4683 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 551 245 164 138 112 42 NaeI GCC'GGC Cuts at: 2418 2418 Size: 5197 NarI GG'CG_CC Cuts at: 2459 2459 Size: 5197 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4645 NdeI CA'TA_TG Cuts at: 387 387 Size: 5197 NgoMIV G'CCGG_C Cuts at: 2416 2416 Size: 5197 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5197 NotI GC'GGCC_GC Cuts at: 2644 2644 Size: 5197 NspI r_CATG'y Cuts at: 1132 1229 2555 4100 1132 Size: 97 1326 1545 2229 Fragments arranged by size: 2229 1545 1326 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5197 PciI A'CATG_T Cuts at: 4096 4096 Size: 5197 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5197 PmeI GTTT'AAAC Cuts at: 2501 2501 Size: 5197 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3266 3298 4779 4811 3266 Size: 32 1481 32 3652 Fragments arranged by size: 3652 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5197 PsiI TTA'TAA Cuts at: 2767 2767 Size: 5197 PspOMI G'GGCC_C Cuts at: 2165 2165 Size: 5197 PstI C_TGCA'G Cuts at: 830 2549 830 Size: 1719 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5197 PvuII CAG'CTG Cuts at: 2407 2449 2407 Size: 42 5155 SalI G'TCGA_C Cuts at: 1936 2539 1936 Size: 603 4594 SbfI CC_TGCA'GG Cuts at: 2549 2549 Size: 5197 ScaI AGT'ACT Cuts at: 3445 5157 3445 Size: 1712 3485 SfcI C'TryA_G Cuts at: 826 1045 2545 3680 4361 4552 826 Size: 219 1500 1135 681 191 1471 Fragments arranged by size: 1500 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2460 2460 Size: 5197 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5197 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5197 SmaI CCC'GGG Cuts at: 1872 2143 2524 1872 Size: 271 381 4545 Fragments arranged by size: 4545 381 271 SmlI C'TyrA_G Cuts at: 820 1017 1943 3301 4202 4464 820 Size: 197 926 1358 901 262 1553 Fragments arranged by size: 1553 1358 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2988 493 Size: 2495 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5197 SphI G_CATG'C Cuts at: 1132 2555 1132 Size: 1423 3774 SspI AAT'ATT Cuts at: 5 52 3121 5 Size: 47 3069 2081 Fragments arranged by size: 3069 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5197 StyI C'CwwG_G Cuts at: 513 1065 2636 513 Size: 552 1571 3074 Fragments arranged by size: 3074 1571 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 3224 3241 3394 3579 4024 325 Size: 1255 774 870 17 153 185 445 1498 Fragments arranged by size: 1498 1255 870 774 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3443 5155 96 Size: 274 80 33 51 1443 1466 1712 138 Fragments arranged by size: 1712 1466 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5157 5189 5157 Size: 32 5165 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5197 XbaI T'CTAG_A Cuts at: 2533 2533 Size: 5197 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2107 2107 Size: 5197 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5197 XmaI C'CCGG_G Cuts at: 1870 2141 2522 1870 Size: 271 381 4545 Fragments arranged by size: 4545 381 271 XmnI GAAnn'nnTTC Cuts at: 3326 3326 Size: 5197 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3005 276 Size: 53 83 186 936 1471 2468 Fragments arranged by size: 2468 1471 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI