Restriction map for
pFN21AE2682
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII AccI AccII AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AviII BalI BamHI BbeI BbrPI
BclI BfrI BglI BlpI Bpu1102I
BsaAI BsaBI BsiWI BsmI Bsp1407I
BspDI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HpaI KasI
KspI MamI MluI MluNI MscI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 1702 GGTACC G^GTACC
AclI 1952 AACGTT AA^CGTT
AcyI 289 GGCGTC G[GA]^CG[CT]C
AflIII 1367 ACATGT A^C[GA][CT]GT
AlwNI 439 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1350 GGGCCC GGGCC^C
Asp718I 1702 GGTACC G^GTACC
AspHI 1183 GAGCTC G[AT]GC[AT]^C
AvrII 702 CCTAGG C^CTAGG
BbvCI 1968 CCTCAGC CCTCAGC(-5/-2)
BcgI 691 CGAGAAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 702 CCTAGG C^CTAGG
BsaHI 289 GGCGTC G[GA]^CG[CT]C
BsaI 1016 GAGACC GGTCTC(1/5)
BsiEI 687 CGACCG CG[GA][CT]^CG
BsiHKAI 1183 GAGCTC G[AT]GC[AT]^C
BsmBI 669 GAGACG CGTCTC(1/5)
BspLU11I 1367 ACATGT A^CATGT
BssSI 302 CTCGTG CACGAG(-5/-1)
BstAPI 853 GCAATGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1750 GGTGACC G^GT[ACGT]ACC
BstPI 1750 GGTGACC G^GT[ACGT]ACC
DraIII 1430 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1190 CCATGG C^C[GA][CT]GG
EarI 1845 GAAGAG CTCTTC(1/4)
EcoO65I 1750 GGTGACC G^GT[ACGT]ACC
EcoRI 175 GAATTC G^AATTC
FauI 676 GCGGG CCCGC(4/6)
Hin1I 289 GGCGTC G[GA]^CG[CT]C
KpnI 1702 GGTACC GGTAC^C
Ksp632I 1845 GAAGAG CTCTTC(1/4)
MslI 252 CATACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1253 CAGCTG C[AC]G^C[GT]G
NcoI 1190 CCATGG C^CATGG
Psp1406I 1952 AACGTT AA^CGTT
PstI 1661 CTGCAG CTGCA^G
PvuII 1253 CAGCTG CAG^CTG
SacI 1183 GAGCTC GAGCT^C
SspI 1197 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccIII 424 TCCGGA T^CCGGA
1324 TCCGGA
Asp700I 106 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1068 GAATGATTTC
AvaI 614 CCCGGG C^[CT]CG[GA]G
1617 CCCGGG
BciVI 421 GTATCC GTATCC(6/5)
707 GTATCC
BfaI 622 CTAG C^TAG
703 CTAG
BsaWI 424 TCCGGA [AT]^CCGG[AT]
1324 TCCGGA
BseAI 424 TCCGGA T^CCGGA
1324 TCCGGA
BseRI 1036 GAGGAG GAGGAG(10/8)
1180 GAGGAG
BsgI 750 GTGCAG GTGCAG(16/14)
860 GTGCAG
BsmFI 1132 GTCCC GGGAC(10/14)
1965 GTCCC
BsoBI 614 CCCGGG C^[CT]CG[GA]G
1617 CCCGGG
BspEI 424 TCCGGA T^CCGGA
1324 TCCGGA
BsrDI 215 GCAATG GCAATG(2/0)
853 GCAATG
CfoI 1614 GCGC GCG^C
1778 GCGC
EcoNI 1029 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1821 CCTGAGACAGG
EcoT14I 702 CCTAGG C^C[AT][AT]GG
1190 CCATGG
HgaI 261 GCGTC GACGC(5/10)
290 GCGTC
HhaI 1614 GCGC GCG^C
1778 GCGC
HinP1I 1614 GCGC G^CGC
1778 GCGC
MaeI 622 CTAG C^TAG
703 CTAG
MfeI 359 CAATTG C^AATTG
1742 CAATTG
MroI 424 TCCGGA T^CCGGA
1324 TCCGGA
MunI 359 CAATTG C^AATTG
1742 CAATTG
PflMI 279 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
328 CCAGGCTGTGG
PpuMI 617 GGGTCCT [GA]G^G[AT]CC[CT]
1259 GGGTCCT
SmaI 614 CCCGGG CCC^GGG
1617 CCCGGG
SmlI 770 CTCAAG C^T[CT][GA]AG
1870 CTCAAG
StyI 702 CCTAGG C^C[AT][AT]GG
1190 CCATGG
Van91I 279 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
328 CCAGGCTGTGG
XmaCI 614 CCCGGG C^CCGGG
1617 CCCGGG
XmaI 614 CCCGGG C^CCGGG
1617 CCCGGG
XmnI 106 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1068 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BanII BbsI
BbvI BcnI BglII BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII