(Circular) (Six-base) MAPSORT of: pFN21AE2682.seq Check: 3719 from: 1 to: 6682 pFN21AE2682 6682 bp With 182 enzymes: SgfI * March 10, 2017 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4492 278 Size: 53 83 186 936 2956 2468 Fragments arranged by size: 2956 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4025 1937 Size: 2088 4594 Acc65I G'GTAC_C Cuts at: 3707 4003 3707 Size: 296 6386 AclI AA'CG_TT Cuts at: 1563 3958 4809 5182 1563 Size: 2395 851 373 3063 Fragments arranged by size: 3063 2395 851 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 4745 6129 6397 1632 Size: 470 2643 1384 268 1917 Fragments arranged by size: 2643 1917 1384 470 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6485 AflIII A'CryG_T Cuts at: 1203 3372 5581 6558 1203 Size: 2169 2209 977 1327 Fragments arranged by size: 2209 2169 1327 977 AgeI A'CCGG_T Cuts at: 5513 5513 Size: 6682 AhdI GACnn_n'nnGTC Cuts at: 5411 5411 Size: 6682 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6682 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6682 AlwNI CAG_nnn'CTG Cuts at: 2449 4425 5997 2449 Size: 1976 1572 3134 Fragments arranged by size: 3134 1976 1572 ApaI G_GGCC'C Cuts at: 3359 3359 Size: 6682 ApaLI G'TGCA_C Cuts at: 4739 5895 4739 Size: 1156 5526 ApoI r'AATT_y Cuts at: 1493 2180 3337 3736 3991 4217 4369 6478 1493 Size: 687 1157 399 255 226 152 2109 1697 Fragments arranged by size: 2109 1697 1157 687 399 255 226 152 AseI AT'TA_AT Cuts at: 160 1032 5236 160 Size: 872 4204 1606 Fragments arranged by size: 4204 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 AvaI C'yCGr_G Cuts at: 1870 1943 2619 3622 4007 1870 Size: 73 676 1003 385 4545 Fragments arranged by size: 4545 1003 676 385 73 AvrII C'CTAG_G Cuts at: 2707 2707 Size: 6682 BamHI G'GATC_C Cuts at: 4012 4379 4012 Size: 367 6315 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3164 3208 3707 4003 5458 Size: 325 217 627 1377 44 499 296 1455 Cuts at: 5458 618 Size: 1842 Fragments arranged by size: 1842 1455 1377 627 499 325 296 217 44 BanII G_rGCy'C Cuts at: 1391 2973 3192 3359 4001 1391 Size: 1582 219 167 642 4072 Fragments arranged by size: 4072 1582 642 219 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2016 2351 3180 3302 928 Size: 971 117 335 829 122 4308 Fragments arranged by size: 4308 971 829 335 122 117 BbvCI CC'TCA_GC Cuts at: 3974 3974 Size: 6682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2685 2719 4873 4907 1903 Size: 34 748 34 2154 34 3678 Fragments arranged by size: 3678 2154 748 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2437 2723 4574 5784 2437 Size: 286 1851 1210 3335 Fragments arranged by size: 3335 1851 1210 286 BclI T'GATC_A Cuts at: 1548 1548 Size: 6682 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5293 136 Size: 107 122 71 631 605 3621 1525 Fragments arranged by size: 3621 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2113 2187 3787 6677 2113 Size: 74 1600 2890 2118 Fragments arranged by size: 2890 2118 1600 74 BlpI GC'TnA_GC Cuts at: 4099 4099 Size: 6682 Bme1580I G_kGCm'C Cuts at: 1790 2304 3169 3359 3826 4743 5899 1790 Size: 514 865 190 467 917 1156 2573 Fragments arranged by size: 2573 1156 917 865 514 467 190 BmgBI CAC'GTC Cuts at: 1318 2678 1318 Size: 1360 5322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4413 5371 442 Size: 416 53 83 399 24 2996 958 1753 Fragments arranged by size: 2996 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6682 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2790 3206 2790 Size: 416 6266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2391 2442 2796 3240 5342 1239 Size: 1152 51 354 444 2102 2579 Fragments arranged by size: 2579 2102 1152 444 354 51 Bpu10I CC'TnA_GC Cuts at: 3974 3974 Size: 6682 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2760 3860 4807 5672 5970 2760 Size: 1100 947 865 298 3472 Fragments arranged by size: 3472 1100 947 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3015 5345 882 Size: 617 1516 2330 2219 Fragments arranged by size: 2330 2219 1516 617 BsaAI yAC'GTr Cuts at: 493 1204 4473 493 Size: 711 3269 2702 Fragments arranged by size: 3269 2702 711 BsaBI GATnn'nnATC Cuts at: 6676 6676 Size: 6682 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2295 4489 4871 5506 Size: 53 83 186 936 762 2194 382 635 Cuts at: 5506 275 Size: 1451 Fragments arranged by size: 2194 1451 936 762 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2429 3329 5115 5513 5787 5934 6345 2429 Size: 900 1786 398 274 147 411 2766 Fragments arranged by size: 2766 1786 900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6457 Fragments arranged by size: 6457 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3056 3200 1186 Size: 1870 144 4668 Fragments arranged by size: 4668 1870 144 BseYI C'CCAG_C Cuts at: 2369 2959 3007 3260 5885 6381 2369 Size: 590 48 253 2625 496 2670 Fragments arranged by size: 2670 2625 590 496 253 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2776 2886 1163 Size: 573 1040 110 4959 Fragments arranged by size: 4959 1040 573 110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2695 4132 4893 5921 1540 Size: 275 186 694 1437 761 1028 2301 Fragments arranged by size: 2301 1437 1028 761 694 275 186 BsiHKAI G_wGCw'C Cuts at: 3192 4743 4828 5899 3192 Size: 1551 85 1071 3975 Fragments arranged by size: 3975 1551 1071 85 BsmI GAATG_Cn' Cuts at: 4193 4286 4193 Size: 93 6589 BsmBI CGTCTCn'nnnn_ Cuts at: 2668 2668 Size: 6682 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2973 3169 3192 3359 3826 4001 Size: 399 514 669 196 23 167 467 175 Cuts at: 4001 4743 4828 5899 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 669 514 467 399 196 175 167 85 23 BspEI T'CCGG_A Cuts at: 2429 3329 6345 2429 Size: 900 3016 2766 Fragments arranged by size: 3016 2766 900 BspHI T'CATG_A Cuts at: 4571 6301 4571 Size: 1730 4952 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 2591 3553 4037 844 Size: 1547 200 962 484 3489 Fragments arranged by size: 3489 1547 962 484 200 BsrBI CCG'CTC Cuts at: 1394 1823 4569 1394 Size: 429 2746 3507 Fragments arranged by size: 3507 2746 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2227 2865 5177 5351 65 Size: 1183 979 638 2312 174 1396 Fragments arranged by size: 2312 1396 1183 979 638 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5326 5513 1700 Size: 110 3516 187 2869 Fragments arranged by size: 3516 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6682 BssSI C'ACGA_G Cuts at: 2307 4742 5754 2307 Size: 2435 1012 3235 Fragments arranged by size: 3235 2435 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2864 794 Size: 2070 4612 BstBI TT'CG_AA Cuts at: 5486 5486 Size: 6682 BstEII G'GTnAC_C Cuts at: 1184 3755 1184 Size: 2571 4111 BstYI r'GATC_y Cuts at: 1972 2020 2113 2187 3028 3787 4012 4379 4764 Size: 48 93 74 841 759 225 367 385 Cuts at: 4764 4781 6233 6319 6331 6677 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 841 759 385 367 346 225 93 86 74 48 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3195 513 Size: 552 939 1191 4000 Fragments arranged by size: 4000 1191 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5943 Fragments arranged by size: 5943 683 56 BtsI GCAGTG_nn' Cuts at: 4198 4992 5012 4198 Size: 794 20 5868 Fragments arranged by size: 5868 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6682 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6647 DraI TTT'AAA Cuts at: 3986 3986 Size: 6682 DraIII CAC_nnn'GTG Cuts at: 3440 3440 Size: 6682 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5689 809 Size: 507 4373 1802 Fragments arranged by size: 4373 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4129 5018 6647 Size: 54 1425 50 275 18 2299 889 1629 Cuts at: 6647 8 Size: 43 Fragments arranged by size: 2299 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4129 1537 Size: 275 2317 4090 Fragments arranged by size: 4090 2317 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3845 4612 1353 Size: 2492 767 3423 Fragments arranged by size: 3423 2492 767 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5269 5643 5789 387 Size: 1064 138 3680 374 146 1280 Fragments arranged by size: 3680 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3190 3190 Size: 6682 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2391 2442 2796 3240 4745 5342 Size: 393 470 289 51 354 444 1505 597 Cuts at: 5342 6129 6397 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1505 787 597 470 444 393 354 289 268 51 EcoNI CCTnn'n_nnAGG Cuts at: 3038 3830 3038 Size: 792 5890 EcoO109I rG'GnC_Cy Cuts at: 2623 3265 3355 3356 2623 Size: 642 90 1 5949 Fragments arranged by size: 5949 642 90 1 EcoRI G'AATT_C Cuts at: 2180 3991 4369 2180 Size: 1811 378 4493 Fragments arranged by size: 4493 1811 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2132 2132 Size: 6682 FspI TGC'GCA Cuts at: 5188 5188 Size: 6682 HaeII r_GCGC'y Cuts at: 1678 5829 1678 Size: 4151 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 2711 2743 2918 Size: 32 135 32 830 32 277 32 175 Cuts at: 2918 2950 3020 3052 3529 3561 5323 5355 5397 Size: 32 70 32 477 32 1762 32 42 Cuts at: 5397 5429 1373 Size: 32 2626 Fragments arranged by size: 2626 1762 830 477 277 175 135 70 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4026 4272 669 Size: 988 281 2088 246 3079 Fragments arranged by size: 3079 2088 988 281 246 HindIII A'AGCT_T Cuts at: 748 2778 3810 3903 748 Size: 2030 1032 93 3527 Fragments arranged by size: 3527 2030 1032 93 HpaI GTT'AAC Cuts at: 4272 4272 Size: 6682 KpnI G_GTAC'C Cuts at: 3711 4007 3711 Size: 296 6386 MfeI C'AATT_G Cuts at: 2364 3747 4281 2364 Size: 1383 534 4765 Fragments arranged by size: 4765 1383 534 MluI A'CGCG_T Cuts at: 6558 6558 Size: 6682 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2217 3127 5796 5980 1305 Size: 912 910 2669 184 2007 Fragments arranged by size: 2669 2007 912 910 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4860 Fragments arranged by size: 4860 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2261 4640 4999 5158 518 Size: 648 1095 2379 359 159 2042 Fragments arranged by size: 2379 2042 1095 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3260 4098 4775 5923 6168 1606 Size: 1654 838 677 1148 245 2120 Fragments arranged by size: 2120 1654 1148 838 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3195 513 Size: 552 939 1191 4000 Fragments arranged by size: 4000 1191 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6682 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6682 NotI GC'GGCC_GC Cuts at: 4129 4129 Size: 6682 NspI r_CATG'y Cuts at: 1132 1229 2266 2363 3376 4040 5585 1132 Size: 97 1037 97 1013 664 1545 2229 Fragments arranged by size: 2229 1545 1037 1013 664 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6682 PciI A'CATG_T Cuts at: 3372 5581 3372 Size: 2209 4473 PflMI CCAn_nnn'nTGG Cuts at: 1108 2290 2339 1108 Size: 1182 49 5451 Fragments arranged by size: 5451 1182 49 PfoI T'CCnGG_A Cuts at: 2946 2946 Size: 6682 PmeI GTTT'AAAC Cuts at: 3986 3986 Size: 6682 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4751 4783 6264 6296 4751 Size: 32 1481 32 5137 Fragments arranged by size: 5137 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2623 3265 2623 Size: 642 6040 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6682 PsiI TTA'TAA Cuts at: 4252 4252 Size: 6682 PspOMI G'GGCC_C Cuts at: 3355 3355 Size: 6682 PstI C_TGCA'G Cuts at: 830 3670 4034 830 Size: 2840 364 3478 Fragments arranged by size: 3478 2840 364 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6682 PvuII CAG'CTG Cuts at: 3260 3260 Size: 6682 SacI G_AGCT'C Cuts at: 3192 3192 Size: 6682 SalI G'TCGA_C Cuts at: 1936 4024 1936 Size: 2088 4594 SbfI CC_TGCA'GG Cuts at: 4034 4034 Size: 6682 ScaI AGT'ACT Cuts at: 4930 6642 4930 Size: 1712 4970 SfcI C'TryA_G Cuts at: 826 1045 2613 2891 3666 4030 5165 5846 6037 Size: 219 1568 278 775 364 1135 681 191 Cuts at: 6037 826 Size: 1471 Fragments arranged by size: 1568 1471 1135 775 681 364 278 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6682 SmaI CCC'GGG Cuts at: 1872 2621 3624 4009 1872 Size: 749 1003 385 4545 Fragments arranged by size: 4545 1003 749 385 SmlI C'TyrA_G Cuts at: 820 1017 1943 2775 3875 4786 5687 5949 820 Size: 197 926 832 1100 911 901 262 1553 Fragments arranged by size: 1553 1100 926 911 901 832 262 197 SnaBI TAC'GTA Cuts at: 493 4473 493 Size: 3980 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6682 SphI G_CATG'C Cuts at: 1132 4040 1132 Size: 2908 3774 SrfI GCCC'GGGC Cuts at: 3624 3624 Size: 6682 SspI AAT'ATT Cuts at: 5 52 3204 4606 5 Size: 47 3152 1402 2081 Fragments arranged by size: 3152 2081 1402 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6682 StyI C'CwwG_G Cuts at: 513 1065 2004 2707 3195 4121 513 Size: 552 939 703 488 926 3074 Fragments arranged by size: 3074 939 926 703 552 488 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2709 3504 4709 4726 4879 5064 5509 Size: 1255 1129 795 1205 17 153 185 445 Cuts at: 5509 325 Size: 1498 Fragments arranged by size: 1498 1255 1205 1129 795 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4928 6640 96 Size: 274 80 33 51 1443 2951 1712 138 Fragments arranged by size: 2951 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6642 6674 6642 Size: 32 6650 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6682 XbaI T'CTAG_A Cuts at: 4018 4018 Size: 6682 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2533 3202 3718 2533 Size: 669 516 5497 Fragments arranged by size: 5497 669 516 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6682 XmaI C'CCGG_G Cuts at: 1870 2619 3622 4007 1870 Size: 749 1003 385 4545 Fragments arranged by size: 4545 1003 749 385 XmnI GAAnn'nnTTC Cuts at: 2115 3077 4811 2115 Size: 962 1734 3986 Fragments arranged by size: 3986 1734 962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4490 276 Size: 53 83 186 936 2956 2468 Fragments arranged by size: 2956 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI