(Linear) (Six-base) MAPSORT of: KIEE2682.seq Check: 3823 from: 1 to: 1977 KIEE2682.orf With 182 enzymes: SgfI * March 10, 2017 10:38 .. Acc65I G'GTAC_C Cuts at: 0 1702 1977 Size: 1702 275 AclI AA'CG_TT Cuts at: 0 1953 1977 Size: 1953 24 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 1977 Size: 97 1880 AflIII A'CryG_T Cuts at: 0 1367 1977 Size: 1367 610 AlwNI CAG_nnn'CTG Cuts at: 0 444 1977 Size: 444 1533 ApaI G_GGCC'C Cuts at: 0 1354 1977 Size: 1354 623 ApoI r'AATT_y Cuts at: 0 175 1332 1731 1977 Size: 175 1157 399 246 Fragments arranged by size: 1157 399 246 175 AvaI C'yCGr_G Cuts at: 0 614 1617 1977 Size: 614 1003 360 Fragments arranged by size: 1003 614 360 AvrII C'CTAG_G Cuts at: 0 702 1977 Size: 702 1275 BanI G'GyrC_C Cuts at: 0 1159 1203 1702 1977 Size: 1159 44 499 275 Fragments arranged by size: 1159 499 275 44 BanII G_rGCy'C Cuts at: 0 968 1187 1354 1977 Size: 968 219 167 623 Fragments arranged by size: 968 623 219 167 BbsI GAAGACnn'nnnn_ Cuts at: 0 11 346 1175 1297 1977 Size: 11 335 829 122 680 Fragments arranged by size: 829 680 335 122 11 BbvCI CC'TCA_GC Cuts at: 0 1969 1977 Size: 1969 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 680 714 1977 Size: 680 34 1263 Fragments arranged by size: 1263 680 34 BciVI GTATCCnnnnn_n' Cuts at: 0 432 718 1977 Size: 432 286 1259 Fragments arranged by size: 1259 432 286 BglII A'GATC_T Cuts at: 0 108 182 1782 1977 Size: 108 74 1600 195 Fragments arranged by size: 1600 195 108 74 Bme1580I G_kGCm'C Cuts at: 0 299 1164 1354 1821 1977 Size: 299 865 190 467 156 Fragments arranged by size: 865 467 299 190 156 BmgBI CAC'GTC Cuts at: 0 673 1977 Size: 673 1304 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 785 1201 1977 Size: 785 416 776 Fragments arranged by size: 785 776 416 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 386 437 791 1235 1977 Size: 386 51 354 444 742 Fragments arranged by size: 742 444 386 354 51 Bpu10I CC'TnA_GC Cuts at: 0 1969 1977 Size: 1969 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 755 1855 1977 Size: 755 1100 122 Fragments arranged by size: 1100 755 122 BsaI GGTCTCn'nnnn_ Cuts at: 0 1010 1977 Size: 1010 967 BsaHI Gr'CG_yC Cuts at: 0 290 1977 Size: 290 1687 BsaWI w'CCGG_w Cuts at: 0 424 1324 1977 Size: 424 900 653 Fragments arranged by size: 900 653 424 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1051 1195 1977 Size: 1051 144 782 Fragments arranged by size: 1051 782 144 BseYI C'CCAG_C Cuts at: 0 364 954 1002 1255 1977 Size: 364 590 48 253 722 Fragments arranged by size: 722 590 364 253 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 771 881 1977 Size: 771 110 1096 Fragments arranged by size: 1096 771 110 BsiEI CG_ry'CG Cuts at: 0 690 1977 Size: 690 1287 BsiHKAI G_wGCw'C Cuts at: 0 1187 1977 Size: 1187 790 BsmBI CGTCTCn'nnnn_ Cuts at: 0 663 1977 Size: 663 1314 Bsp1286I G_dGCh'C Cuts at: 0 299 968 1164 1187 1354 1821 1977 Size: 299 669 196 23 167 467 156 Fragments arranged by size: 669 467 299 196 167 156 23 BspEI T'CCGG_A Cuts at: 0 424 1324 1977 Size: 424 900 653 Fragments arranged by size: 900 653 424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 386 586 1548 1977 Size: 386 200 962 429 Fragments arranged by size: 962 429 386 200 BsrDI GCAATG_nn' Cuts at: 0 222 860 1977 Size: 222 638 1117 Fragments arranged by size: 1117 638 222 BssSI C'ACGA_G Cuts at: 0 302 1977 Size: 302 1675 BstAPI GCAn_nnn'nTGC Cuts at: 0 859 1977 Size: 859 1118 BstEII G'GTnAC_C Cuts at: 0 1750 1977 Size: 1750 227 BstYI r'GATC_y Cuts at: 0 15 108 182 1023 1782 1977 Size: 15 93 74 841 759 195 Fragments arranged by size: 841 759 195 93 74 15 BtgI C'CryG_G Cuts at: 0 1190 1977 Size: 1190 787 DraIII CAC_nnn'GTG Cuts at: 0 1435 1977 Size: 1435 542 EarI CTCTTCn'nnn_ Cuts at: 0 1840 1977 Size: 1840 137 EcoICRI GAG'CTC Cuts at: 0 1185 1977 Size: 1185 792 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 386 437 791 1235 1977 Size: 97 289 51 354 444 742 Fragments arranged by size: 742 444 354 289 97 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 1033 1825 1977 Size: 1033 792 152 Fragments arranged by size: 1033 792 152 EcoO109I rG'GnC_Cy Cuts at: 0 618 1260 1350 1351 1977 Size: 618 642 90 1 626 Fragments arranged by size: 642 626 618 90 1 EcoRI G'AATT_C Cuts at: 0 175 1977 Size: 175 1802 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 127 1977 Size: 127 1850 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 397 429 706 738 913 945 1015 1047 Size: 397 32 277 32 175 32 70 32 Cuts at: 1047 1524 1556 1977 Size: 477 32 421 Fragments arranged by size: 477 421 397 277 175 70 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 773 1805 1898 1977 Size: 773 1032 93 79 Fragments arranged by size: 1032 773 93 79 KpnI G_GTAC'C Cuts at: 0 1706 1977 Size: 1706 271 MfeI C'AATT_G Cuts at: 0 359 1742 1977 Size: 359 1383 235 Fragments arranged by size: 1383 359 235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 212 1122 1977 Size: 212 910 855 Fragments arranged by size: 910 855 212 MslI CAynn'nnrTG Cuts at: 0 256 1977 Size: 256 1721 MspA1I CmG'CkG Cuts at: 0 1255 1977 Size: 1255 722 NcoI C'CATG_G Cuts at: 0 1190 1977 Size: 1190 787 NspI r_CATG'y Cuts at: 0 261 358 1371 1977 Size: 261 97 1013 606 Fragments arranged by size: 1013 606 261 97 PciI A'CATG_T Cuts at: 0 1367 1977 Size: 1367 610 PflMI CCAn_nnn'nTGG Cuts at: 0 285 334 1977 Size: 285 49 1643 Fragments arranged by size: 1643 285 49 PfoI T'CCnGG_A Cuts at: 0 941 1977 Size: 941 1036 PpuMI rG'GwC_Cy Cuts at: 0 618 1260 1977 Size: 618 642 717 Fragments arranged by size: 717 642 618 PspOMI G'GGCC_C Cuts at: 0 1350 1977 Size: 1350 627 PstI C_TGCA'G Cuts at: 0 1665 1977 Size: 1665 312 PvuII CAG'CTG Cuts at: 0 1255 1977 Size: 1255 722 SacI G_AGCT'C Cuts at: 0 1187 1977 Size: 1187 790 SfcI C'TryA_G Cuts at: 0 608 886 1661 1977 Size: 608 278 775 316 Fragments arranged by size: 775 608 316 278 SmaI CCC'GGG Cuts at: 0 616 1619 1977 Size: 616 1003 358 Fragments arranged by size: 1003 616 358 SmlI C'TyrA_G Cuts at: 0 770 1870 1977 Size: 770 1100 107 Fragments arranged by size: 1100 770 107 SrfI GCCC'GGGC Cuts at: 0 1619 1977 Size: 1619 358 SspI AAT'ATT Cuts at: 0 1199 1977 Size: 1199 778 StyI C'CwwG_G Cuts at: 0 702 1190 1977 Size: 702 488 787 Fragments arranged by size: 787 702 488 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 704 1499 1977 Size: 704 795 478 Fragments arranged by size: 795 704 478 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 528 1197 1713 1977 Size: 528 669 516 264 Fragments arranged by size: 669 528 516 264 XmaI C'CCGG_G Cuts at: 0 614 1617 1977 Size: 614 1003 360 Fragments arranged by size: 1003 614 360 XmnI GAAnn'nnTTC Cuts at: 0 110 1072 1977 Size: 110 962 905 Fragments arranged by size: 962 905 110 Enzymes that do cut: Acc65I AclI AcuI AflIII AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI BbvCI BcgI BciVI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BssSI BstAPI BstEII BstYI BtgI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HindIII KpnI MfeI MmeI MslI MspA1I NcoI NspI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SfcI SmaI SmlI SrfI SspI StyI TaqII XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BclI BfrBI BglI BlpI BmrI BmtI BsaAI BsaBI BsaXI BsiWI BsmI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoRV FseI FspI FspAI HaeII HincII HpaI KasI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI StuI SwaI TatI TstI Tth111I XbaI XhoI ZraI