(Circular) (Six-base) MAPSORT of: pFN21AE2424.seq Check: 6136 from: 1 to: 7192 pFN21AE2424 7192 bp With 182 enzymes: SgfI * November 9, 2012 16:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5002 278 Size: 53 83 186 936 3466 2468 Fragments arranged by size: 3466 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4535 1937 Size: 2598 4594 Acc65I G'GTAC_C Cuts at: 4513 4513 Size: 7192 AclI AA'CG_TT Cuts at: 1563 5319 5692 1563 Size: 3756 373 3063 Fragments arranged by size: 3756 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 3791 5255 6639 6907 1632 Size: 930 1229 1464 1384 268 1917 Fragments arranged by size: 1917 1464 1384 1229 930 268 AfeI AGC'GCT Cuts at: 3019 3142 3019 Size: 123 7069 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6995 AflIII A'CryG_T Cuts at: 1203 6091 7068 1203 Size: 4888 977 1327 Fragments arranged by size: 4888 1327 977 AgeI A'CCGG_T Cuts at: 2812 6023 2812 Size: 3211 3981 AhdI GACnn_n'nnGTC Cuts at: 5921 5921 Size: 7192 AleI CACnn'nnGTG Cuts at: 1166 2120 4262 1166 Size: 954 2142 4096 Fragments arranged by size: 4096 2142 954 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4082 1184 Size: 2898 4294 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 2372 Size: 32 7160 AlwNI CAG_nnn'CTG Cuts at: 4311 4935 6507 4311 Size: 624 1572 4996 Fragments arranged by size: 4996 1572 624 ApaI G_GGCC'C Cuts at: 2079 3586 2079 Size: 1507 5685 ApaLI G'TGCA_C Cuts at: 5249 6405 5249 Size: 1156 6036 ApoI r'AATT_y Cuts at: 1493 4501 4727 4879 6988 1493 Size: 3008 226 152 2109 1697 Fragments arranged by size: 3008 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5746 160 Size: 872 4714 1606 Fragments arranged by size: 4714 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7192 AvaI C'yCGr_G Cuts at: 1870 1943 3039 3186 3979 4517 1870 Size: 73 1096 147 793 538 4545 Fragments arranged by size: 4545 1096 793 538 147 73 BamHI G'GATC_C Cuts at: 3621 4522 4889 3621 Size: 901 367 5924 Fragments arranged by size: 5924 901 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 2287 2826 3789 4513 Size: 325 217 627 449 51 539 963 724 Cuts at: 4513 5968 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 963 724 627 539 449 325 217 51 BanII G_rGCy'C Cuts at: 1391 2079 2538 2780 3206 3586 4511 1391 Size: 688 459 242 426 380 925 4072 Fragments arranged by size: 4072 925 688 459 426 380 242 BbeI G_GCGC'C Cuts at: 2240 2240 Size: 7192 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2759 2766 2984 3361 3895 4480 928 Size: 971 860 7 218 377 534 585 3640 Fragments arranged by size: 3640 971 860 585 534 377 218 7 BbvCI CC'TCA_GC Cuts at: 3506 3938 3506 Size: 432 6760 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4345 4379 5383 5417 1903 Size: 34 2408 34 1004 34 3678 Fragments arranged by size: 3678 2408 1004 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4453 5084 6294 4453 Size: 631 1210 5351 Fragments arranged by size: 5351 1210 631 BclI T'GATC_A Cuts at: 1548 3399 1548 Size: 1851 5341 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3833 5803 136 Size: 107 122 71 631 605 2161 1970 1525 Fragments arranged by size: 2161 1970 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3336 4479 7187 3336 Size: 1143 2708 3341 Fragments arranged by size: 3341 2708 1143 BlpI GC'TnA_GC Cuts at: 4609 4609 Size: 7192 Bme1580I G_kGCm'C Cuts at: 1790 2079 2292 2829 3586 3610 3659 3707 5253 Size: 289 213 537 757 24 49 48 1546 Cuts at: 5253 6409 1790 Size: 1156 2573 Fragments arranged by size: 2573 1546 1156 757 537 289 213 49 48 24 BmgBI CAC'GTC Cuts at: 1318 2871 1318 Size: 1553 5639 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2487 2512 3159 Size: 416 53 83 399 24 1070 25 647 Cuts at: 3159 3485 4923 5881 442 Size: 326 1438 958 1753 Fragments arranged by size: 1753 1438 1070 958 647 416 399 326 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7192 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2983 5852 1239 Size: 1744 2869 2579 Fragments arranged by size: 2869 2579 1744 Bpu10I CC'TnA_GC Cuts at: 2471 3506 3938 2471 Size: 1035 432 5725 Fragments arranged by size: 5725 1035 432 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2844 5317 6182 6480 2844 Size: 2473 865 298 3556 Fragments arranged by size: 3556 2473 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5855 882 Size: 617 4356 2219 Fragments arranged by size: 4356 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2122 2398 4006 4051 4983 493 Size: 711 918 276 1608 45 932 2702 Fragments arranged by size: 2702 1608 932 918 711 276 45 BsaBI GATnn'nnATC Cuts at: 7186 7186 Size: 7192 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 2237 4999 5381 Size: 53 83 186 936 567 137 2762 382 Cuts at: 5381 6016 275 Size: 635 1451 Fragments arranged by size: 2762 1451 936 635 567 382 186 137 83 53 BsaWI w'CCGG_w Cuts at: 2812 4209 5625 6023 6297 6444 6855 2812 Size: 1397 1416 398 274 147 411 3149 Fragments arranged by size: 3149 1416 1397 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2372 2402 3080 3110 4385 Size: 30 165 30 969 30 678 30 1275 Cuts at: 4385 4415 1178 Size: 30 3955 Fragments arranged by size: 3955 1275 969 678 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3227 3926 4352 4393 1186 Size: 2041 699 426 41 3985 Fragments arranged by size: 3985 2041 699 426 41 BseYI C'CCAG_C Cuts at: 2720 2952 3529 3859 6395 6891 2720 Size: 232 577 330 2536 496 3021 Fragments arranged by size: 3021 2536 577 496 330 232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3410 4054 4487 1163 Size: 573 1674 644 433 3868 Fragments arranged by size: 3868 1674 644 573 433 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2052 2817 4642 5403 6431 1540 Size: 275 186 51 765 1825 761 1028 2301 Fragments arranged by size: 2301 1825 1028 765 761 275 186 51 BsiHKAI G_wGCw'C Cuts at: 2037 2472 2538 3206 4011 4344 5253 5338 6409 Size: 435 66 668 805 333 909 85 1071 Cuts at: 6409 2037 Size: 2820 Fragments arranged by size: 2820 1071 909 805 668 435 333 85 66 BsmI GAATG_Cn' Cuts at: 4703 4796 4703 Size: 93 7099 BsmBI CGTCTCn'nnnn_ Cuts at: 2092 2092 Size: 7192 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2079 2292 2472 2538 2780 2829 Size: 399 247 42 213 180 66 242 49 Cuts at: 2829 3206 3586 3610 3659 3707 4011 4344 4511 Size: 377 380 24 49 48 304 333 167 Cuts at: 4511 5253 5338 6409 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 377 333 304 247 242 213 180 167 85 66 49 49 48 42 24 BspEI T'CCGG_A Cuts at: 4209 6855 4209 Size: 2646 4546 BspHI T'CATG_A Cuts at: 4200 5081 6811 4200 Size: 881 1730 4581 Fragments arranged by size: 4581 1730 881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2937 4179 4547 844 Size: 2093 1242 368 3489 Fragments arranged by size: 3489 2093 1242 368 BsrBI CCG'CTC Cuts at: 1394 1823 4045 5079 1394 Size: 429 2222 1034 3507 Fragments arranged by size: 3507 2222 1034 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3351 3360 3601 5687 5861 65 Size: 1183 2103 9 241 2086 174 1396 Fragments arranged by size: 2103 2086 1396 1183 241 174 9 BsrFI r'CCGG_y Cuts at: 1700 1810 2812 3963 5836 6023 1700 Size: 110 1002 1151 1873 187 2869 Fragments arranged by size: 2869 1873 1151 1002 187 110 BsrGI T'GTAC_A Cuts at: 96 3744 96 Size: 3648 3544 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7192 BssSI C'ACGA_G Cuts at: 5252 6264 5252 Size: 1012 6180 BstAPI GCAn_nnn'nTGC Cuts at: 794 4254 794 Size: 3460 3732 BstBI TT'CG_AA Cuts at: 5996 5996 Size: 7192 BstEII G'GTnAC_C Cuts at: 1184 2569 3032 3167 1184 Size: 1385 463 135 5209 Fragments arranged by size: 5209 1385 463 135 BstYI r'GATC_y Cuts at: 1972 3116 3336 3621 3984 4479 4522 4889 5274 Size: 1144 220 285 363 495 43 367 385 Cuts at: 5274 5291 6743 6829 6841 7187 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1144 495 385 367 363 346 285 220 86 43 17 12 BtgI C'CryG_G Cuts at: 513 1065 3651 4263 513 Size: 552 2586 612 3442 Fragments arranged by size: 3442 2586 612 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3907 4393 487 Size: 683 56 2681 486 3286 Fragments arranged by size: 3286 2681 683 486 56 BtsI GCAGTG_nn' Cuts at: 4037 4708 5502 5522 4037 Size: 671 794 20 5707 Fragments arranged by size: 5707 794 671 20 ClaI AT'CG_AT Cuts at: 1554 3243 1554 Size: 1689 5503 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7157 DraI TTT'AAA Cuts at: 4496 4496 Size: 7192 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3257 6199 809 Size: 507 1941 2942 1802 Fragments arranged by size: 2942 1941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2691 2991 3834 Size: 54 1425 50 275 18 861 300 843 Cuts at: 3834 4152 4639 5528 7157 8 Size: 318 487 889 1629 43 Fragments arranged by size: 1629 1425 889 861 843 487 318 300 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4639 1537 Size: 275 2827 4090 Fragments arranged by size: 4090 2827 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4289 5122 1353 Size: 2936 833 3423 Fragments arranged by size: 3423 2936 833 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2246 3980 5779 6153 6299 387 Size: 1064 138 657 1734 1799 374 146 1280 Fragments arranged by size: 1799 1734 1280 1064 657 374 146 138 EcoICRI GAG'CTC Cuts at: 2536 3204 2536 Size: 668 6524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2562 2983 3791 5255 5852 6639 6907 Size: 393 930 421 808 1464 597 787 268 Cuts at: 6907 1239 Size: 1524 Fragments arranged by size: 1524 1464 930 808 787 597 421 393 268 EcoO109I rG'GnC_Cy Cuts at: 2075 3154 3583 4422 2075 Size: 1079 429 839 4845 Fragments arranged by size: 4845 1079 839 429 EcoRI G'AATT_C Cuts at: 4501 4879 4501 Size: 378 6814 FspI TGC'GCA Cuts at: 3593 5698 3593 Size: 2105 5087 HaeII r_GCGC'y Cuts at: 1678 2240 3021 3144 3492 3813 6339 1678 Size: 562 781 123 348 321 2526 2531 Fragments arranged by size: 2531 2526 781 562 348 321 123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5833 5865 5907 5939 1373 Size: 32 135 32 4261 32 42 32 2626 Fragments arranged by size: 4261 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 2149 2425 3136 4536 4782 Size: 988 281 196 15 276 711 1400 246 Cuts at: 4782 669 Size: 3079 Fragments arranged by size: 3079 1400 988 711 281 276 246 196 15 HindIII A'AGCT_T Cuts at: 748 748 Size: 7192 HpaI GTT'AAC Cuts at: 4782 4782 Size: 7192 KasI G'GCGC_C Cuts at: 2236 2236 Size: 7192 KpnI G_GTAC'C Cuts at: 4517 4517 Size: 7192 MfeI C'AATT_G Cuts at: 4791 4791 Size: 7192 MluI A'CGCG_T Cuts at: 7068 7068 Size: 7192 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2358 2601 3273 6306 6490 1305 Size: 1053 243 672 3033 184 2007 Fragments arranged by size: 3033 2007 1053 672 243 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2993 3836 4154 10 Size: 54 1425 343 1161 843 318 3048 Fragments arranged by size: 3048 1425 1161 843 343 318 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2663 3746 4199 4262 4412 5150 Size: 648 954 543 1083 453 63 150 738 Cuts at: 5150 5509 5668 518 Size: 359 159 2042 Fragments arranged by size: 2042 1083 954 738 648 543 453 359 159 150 63 MspA1I CmG'CkG Cuts at: 1606 3394 4038 4471 4608 5285 6433 6678 1606 Size: 1788 644 433 137 677 1148 245 2120 Fragments arranged by size: 2120 1788 1148 677 644 433 245 137 NarI GG'CG_CC Cuts at: 2237 2237 Size: 7192 NcoI C'CATG_G Cuts at: 513 1065 3651 513 Size: 552 2586 4054 Fragments arranged by size: 4054 2586 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7192 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7192 NotI GC'GGCC_GC Cuts at: 4639 4639 Size: 7192 NspI r_CATG'y Cuts at: 1132 1229 2668 3769 4550 6095 1132 Size: 97 1439 1101 781 1545 2229 Fragments arranged by size: 2229 1545 1439 1101 781 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7192 PciI A'CATG_T Cuts at: 6091 6091 Size: 7192 PflMI CCAn_nnn'nTGG Cuts at: 1108 2338 3415 3475 1108 Size: 1230 1077 60 4825 Fragments arranged by size: 4825 1230 1077 60 PmeI GTTT'AAAC Cuts at: 4496 4496 Size: 7192 PmlI CAC'GTG Cuts at: 4006 4006 Size: 7192 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2372 2404 5261 5293 6774 6806 2372 Size: 32 2857 32 1481 32 2758 Fragments arranged by size: 2857 2758 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3154 4422 3154 Size: 1268 5924 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7192 PsiI TTA'TAA Cuts at: 4762 4762 Size: 7192 PspOMI G'GGCC_C Cuts at: 2075 3582 2075 Size: 1507 5685 PspXI vC'TCGA_Gb Cuts at: 3039 3039 Size: 7192 PstI C_TGCA'G Cuts at: 830 4330 4544 830 Size: 3500 214 3478 Fragments arranged by size: 3500 3478 214 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7192 PvuII CAG'CTG Cuts at: 3394 3394 Size: 7192 SacI G_AGCT'C Cuts at: 2538 3206 2538 Size: 668 6524 SalI G'TCGA_C Cuts at: 1936 4534 1936 Size: 2598 4594 SapI GCTCTTCn'nnn_ Cuts at: 4289 4289 Size: 7192 SbfI CC_TGCA'GG Cuts at: 4544 4544 Size: 7192 ScaI AGT'ACT Cuts at: 2522 5440 7152 2522 Size: 2918 1712 2562 Fragments arranged by size: 2918 2562 1712 SfcI C'TryA_G Cuts at: 826 1045 3814 4326 4540 5675 6356 6547 826 Size: 219 2769 512 214 1135 681 191 1471 Fragments arranged by size: 2769 1471 1135 681 512 219 214 191 SfoI GGC'GCC Cuts at: 2238 2238 Size: 7192 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7192 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7192 SmaI CCC'GGG Cuts at: 1872 4519 1872 Size: 2647 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2859 3039 5296 6197 6459 820 Size: 197 926 916 180 2257 901 262 1553 Fragments arranged by size: 2257 1553 926 916 901 262 197 180 SnaBI TAC'GTA Cuts at: 493 4983 493 Size: 4490 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7192 SphI G_CATG'C Cuts at: 1132 3769 4550 1132 Size: 2637 781 3774 Fragments arranged by size: 3774 2637 781 SspI AAT'ATT Cuts at: 5 52 5116 5 Size: 47 5064 2081 Fragments arranged by size: 5064 2081 47 StuI AGG'CCT Cuts at: 1517 2585 1517 Size: 1068 6124 StyI C'CwwG_G Cuts at: 513 1065 3028 3651 4631 513 Size: 552 1963 623 980 3074 Fragments arranged by size: 3074 1963 980 623 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 2771 3015 3251 3486 5219 5236 Size: 1255 458 733 244 236 235 1733 17 Cuts at: 5236 5389 5574 6019 325 Size: 153 185 445 1498 Fragments arranged by size: 1733 1498 1255 733 458 445 244 236 235 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 3744 5438 Size: 274 80 33 51 1443 543 1224 1694 Cuts at: 5438 7150 96 Size: 1712 138 Fragments arranged by size: 1712 1694 1443 1224 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2606 2638 4385 4417 7152 7184 2606 Size: 32 1747 32 2735 32 2614 Fragments arranged by size: 2735 2614 1747 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2129 1316 Size: 813 6379 XbaI T'CTAG_A Cuts at: 4528 4528 Size: 7192 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3205 2011 Size: 1194 5998 XhoI C'TCGA_G Cuts at: 1943 3039 1943 Size: 1096 6096 XmaI C'CCGG_G Cuts at: 1870 4517 1870 Size: 2647 4545 XmnI GAAnn'nnTTC Cuts at: 5321 5321 Size: 7192 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5000 276 Size: 53 83 186 936 3466 2468 Fragments arranged by size: 3466 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI