(Linear) (Six-base) MAPSORT of: KIEE2424.seq Check: 3166 from: 1 to: 2487 KIEE2424.orf With 182 enzymes: SgfI * November 9, 2012 16:00 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 557 1786 2487 Size: 557 1229 701 Fragments arranged by size: 1229 701 557 AfeI AGC'GCT Cuts at: 0 1014 1137 2487 Size: 1014 123 1350 Fragments arranged by size: 1350 1014 123 AgeI A'CCGG_T Cuts at: 0 807 2487 Size: 807 1680 AleI CACnn'nnGTG Cuts at: 0 115 2257 2487 Size: 115 2142 230 Fragments arranged by size: 2142 230 115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2077 2487 Size: 2077 410 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 367 399 2487 Size: 367 32 2088 Fragments arranged by size: 2088 367 32 AlwNI CAG_nnn'CTG Cuts at: 0 2306 2487 Size: 2306 181 ApaI G_GGCC'C Cuts at: 0 74 1581 2487 Size: 74 1507 906 Fragments arranged by size: 1507 906 74 AvaI C'yCGr_G Cuts at: 0 1034 1181 1974 2487 Size: 1034 147 793 513 Fragments arranged by size: 1034 793 513 147 BamHI G'GATC_C Cuts at: 0 1616 2487 Size: 1616 871 BanI G'GyrC_C Cuts at: 0 231 282 821 1784 2487 Size: 231 51 539 963 703 Fragments arranged by size: 963 703 539 231 51 BanII G_rGCy'C Cuts at: 0 74 533 775 1201 1581 2487 Size: 74 459 242 426 380 906 Fragments arranged by size: 906 459 426 380 242 74 BbeI G_GCGC'C Cuts at: 0 235 2487 Size: 235 2252 BbsI GAAGACnn'nnnn_ Cuts at: 0 754 761 979 1356 1890 2475 2487 Size: 754 7 218 377 534 585 12 Fragments arranged by size: 754 585 534 377 218 12 7 BbvCI CC'TCA_GC Cuts at: 0 1501 1933 2487 Size: 1501 432 554 Fragments arranged by size: 1501 554 432 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2340 2374 2487 Size: 2340 34 113 Fragments arranged by size: 2340 113 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2448 2487 Size: 2448 39 BclI T'GATC_A Cuts at: 0 1394 2487 Size: 1394 1093 BglI GCCn_nnn'nGGC Cuts at: 0 1828 2487 Size: 1828 659 BglII A'GATC_T Cuts at: 0 1331 2474 2487 Size: 1331 1143 13 Fragments arranged by size: 1331 1143 13 Bme1580I G_kGCm'C Cuts at: 0 74 287 824 1581 1605 1654 1702 2487 Size: 74 213 537 757 24 49 48 785 Fragments arranged by size: 785 757 537 213 74 49 48 24 BmgBI CAC'GTC Cuts at: 0 866 2487 Size: 866 1621 BmrI ACTGGGnnnn_n' Cuts at: 0 482 507 1154 1480 2487 Size: 482 25 647 326 1007 Fragments arranged by size: 1007 647 482 326 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 978 2487 Size: 978 1509 Bpu10I CC'TnA_GC Cuts at: 0 466 1501 1933 2487 Size: 466 1035 432 554 Fragments arranged by size: 1035 554 466 432 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 839 2487 Size: 839 1648 BsaAI yAC'GTr Cuts at: 0 117 393 2001 2046 2487 Size: 117 276 1608 45 441 Fragments arranged by size: 1608 441 276 117 45 BsaHI Gr'CG_yC Cuts at: 0 95 232 2487 Size: 95 137 2255 Fragments arranged by size: 2255 137 95 BsaWI w'CCGG_w Cuts at: 0 807 2204 2487 Size: 807 1397 283 Fragments arranged by size: 1397 807 283 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 367 397 1075 1105 2380 2410 2487 Size: 367 30 678 30 1275 30 77 Fragments arranged by size: 1275 678 367 77 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1222 1921 2347 2388 2487 Size: 1222 699 426 41 99 Fragments arranged by size: 1222 699 426 99 41 BseYI C'CCAG_C Cuts at: 0 715 947 1524 1854 2487 Size: 715 232 577 330 633 Fragments arranged by size: 715 633 577 330 232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1405 2049 2482 2487 Size: 1405 644 433 5 Fragments arranged by size: 1405 644 433 5 BsiEI CG_ry'CG Cuts at: 0 47 812 2487 Size: 47 765 1675 Fragments arranged by size: 1675 765 47 BsiHKAI G_wGCw'C Cuts at: 0 32 467 533 1201 2006 2339 2487 Size: 32 435 66 668 805 333 148 Fragments arranged by size: 805 668 435 333 148 66 32 BsmBI CGTCTCn'nnnn_ Cuts at: 0 87 2487 Size: 87 2400 Bsp1286I G_dGCh'C Cuts at: 0 32 74 287 467 533 775 824 1201 Size: 32 42 213 180 66 242 49 377 Cuts at: 1201 1581 1605 1654 1702 2006 2339 2487 Size: 380 24 49 48 304 333 148 Fragments arranged by size: 380 377 333 304 242 213 180 148 66 49 49 48 42 32 24 BspEI T'CCGG_A Cuts at: 0 2204 2487 Size: 2204 283 BspHI T'CATG_A Cuts at: 0 2195 2487 Size: 2195 292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 932 2174 2487 Size: 932 1242 313 Fragments arranged by size: 1242 932 313 BsrBI CCG'CTC Cuts at: 0 2040 2487 Size: 2040 447 BsrDI GCAATG_nn' Cuts at: 0 1346 1355 1596 2487 Size: 1346 9 241 891 Fragments arranged by size: 1346 891 241 9 BsrFI r'CCGG_y Cuts at: 0 807 1958 2487 Size: 807 1151 529 Fragments arranged by size: 1151 807 529 BsrGI T'GTAC_A Cuts at: 0 1739 2487 Size: 1739 748 BstAPI GCAn_nnn'nTGC Cuts at: 0 2249 2487 Size: 2249 238 BstEII G'GTnAC_C Cuts at: 0 564 1027 1162 2487 Size: 564 463 135 1325 Fragments arranged by size: 1325 564 463 135 BstYI r'GATC_y Cuts at: 0 1111 1331 1616 1979 2474 2487 Size: 1111 220 285 363 495 13 Fragments arranged by size: 1111 495 363 285 220 13 BtgI C'CryG_G Cuts at: 0 1646 2258 2487 Size: 1646 612 229 Fragments arranged by size: 1646 612 229 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1902 2388 2487 Size: 1902 486 99 Fragments arranged by size: 1902 486 99 BtsI GCAGTG_nn' Cuts at: 0 2032 2487 Size: 2032 455 ClaI AT'CG_AT Cuts at: 0 1238 2487 Size: 1238 1249 DrdI GACnn_nn'nnGTC Cuts at: 0 1252 2487 Size: 1252 1235 EaeI y'GGCC_r Cuts at: 0 686 986 1829 2147 2487 Size: 686 300 843 318 340 Fragments arranged by size: 843 686 340 318 300 EarI CTCTTCn'nnn_ Cuts at: 0 2284 2487 Size: 2284 203 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 241 1975 2487 Size: 241 1734 512 Fragments arranged by size: 1734 512 241 EcoICRI GAG'CTC Cuts at: 0 531 1199 2487 Size: 531 668 1288 Fragments arranged by size: 1288 668 531 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 557 978 1786 2487 Size: 557 421 808 701 Fragments arranged by size: 808 701 557 421 EcoO109I rG'GnC_Cy Cuts at: 0 70 1149 1578 2417 2487 Size: 70 1079 429 839 70 Fragments arranged by size: 1079 839 429 70 70 FspI TGC'GCA Cuts at: 0 1588 2487 Size: 1588 899 HaeII r_GCGC'y Cuts at: 0 235 1016 1139 1487 1808 2487 Size: 235 781 123 348 321 679 Fragments arranged by size: 781 679 348 321 235 123 HincII GTy'rAC Cuts at: 0 129 144 420 1131 2487 Size: 129 15 276 711 1356 Fragments arranged by size: 1356 711 276 129 15 KasI G'GCGC_C Cuts at: 0 231 2487 Size: 231 2256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 353 596 1268 2487 Size: 353 243 672 1219 Fragments arranged by size: 1219 672 353 243 MscI TGG'CCA Cuts at: 0 988 1831 2149 2487 Size: 988 843 318 338 Fragments arranged by size: 988 843 338 318 MslI CAynn'nnrTG Cuts at: 0 115 658 1741 2194 2257 2407 2487 Size: 115 543 1083 453 63 150 80 Fragments arranged by size: 1083 543 453 150 115 80 63 MspA1I CmG'CkG Cuts at: 0 1389 2033 2466 2487 Size: 1389 644 433 21 Fragments arranged by size: 1389 644 433 21 NarI GG'CG_CC Cuts at: 0 232 2487 Size: 232 2255 NcoI C'CATG_G Cuts at: 0 1646 2487 Size: 1646 841 NspI r_CATG'y Cuts at: 0 663 1764 2487 Size: 663 1101 723 Fragments arranged by size: 1101 723 663 PflMI CCAn_nnn'nTGG Cuts at: 0 333 1410 1470 2487 Size: 333 1077 60 1017 Fragments arranged by size: 1077 1017 333 60 PmlI CAC'GTG Cuts at: 0 2001 2487 Size: 2001 486 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 367 399 2487 Size: 367 32 2088 Fragments arranged by size: 2088 367 32 PpuMI rG'GwC_Cy Cuts at: 0 1149 2417 2487 Size: 1149 1268 70 Fragments arranged by size: 1268 1149 70 PspOMI G'GGCC_C Cuts at: 0 70 1577 2487 Size: 70 1507 910 Fragments arranged by size: 1507 910 70 PspXI vC'TCGA_Gb Cuts at: 0 1034 2487 Size: 1034 1453 PstI C_TGCA'G Cuts at: 0 2325 2487 Size: 2325 162 PvuII CAG'CTG Cuts at: 0 1389 2487 Size: 1389 1098 SacI G_AGCT'C Cuts at: 0 533 1201 2487 Size: 533 668 1286 Fragments arranged by size: 1286 668 533 SapI GCTCTTCn'nnn_ Cuts at: 0 2284 2487 Size: 2284 203 ScaI AGT'ACT Cuts at: 0 517 2487 Size: 517 1970 SfcI C'TryA_G Cuts at: 0 1809 2321 2487 Size: 1809 512 166 Fragments arranged by size: 1809 512 166 SfoI GGC'GCC Cuts at: 0 233 2487 Size: 233 2254 SmlI C'TyrA_G Cuts at: 0 854 1034 2487 Size: 854 180 1453 Fragments arranged by size: 1453 854 180 SphI G_CATG'C Cuts at: 0 1764 2487 Size: 1764 723 StuI AGG'CCT Cuts at: 0 580 2487 Size: 580 1907 StyI C'CwwG_G Cuts at: 0 1023 1646 2487 Size: 1023 623 841 Fragments arranged by size: 1023 841 623 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 33 766 1010 1246 1481 2487 Size: 33 733 244 236 235 1006 Fragments arranged by size: 1006 733 244 236 235 33 TatI w'GTAC_w Cuts at: 0 515 1739 2487 Size: 515 1224 748 Fragments arranged by size: 1224 748 515 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 601 633 2380 2412 2487 Size: 601 32 1747 32 75 Fragments arranged by size: 1747 601 75 32 32 Tth111I GACn'n_nGTC Cuts at: 0 124 2487 Size: 124 2363 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1200 2487 Size: 1200 1287 XhoI C'TCGA_G Cuts at: 0 1034 2487 Size: 1034 1453 Enzymes that do cut: AcuI AfeI AgeI AleI AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstYI BtgI BtgZI BtsI ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII HincII KasI MmeI MscI MslI MspA1I NarI NcoI NspI PflMI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SapI ScaI SfcI SfoI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AhdI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BfrBI BlpI BmtI BplI BsaI BsaBI BsiWI BsmI BssHII BssSI BstBI BstXI BstZ17I Bsu36I CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI XmaI XmnI ZraI