################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BbsI BcgI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AcyI 79 GGCGCC G[GA]^CG[CT]C BamHI 546 GGATCC G^GATCC BbeI 79 GGCGCC GGCGC^C BciVI 271 GGATAC GTATCC(6/5) BclI 416 TGATCA T^GATCA BglII 494 AGATCT A^GATCT BsaAI 727 TACGTG [CT]AC^GT[GA] BsaHI 79 GGCGCC G[GA]^CG[CT]C BsaI 177 GGTCTC GGTCTC(1/5) BsmBI 706 CGTCTC CGTCTC(1/5) Bsp1407I 641 TGTACA T^GTACA BspHI 1986 TCATGA T^CATGA BsrBI 793 GAGCGG CCGCTC(-3/-3) BsrDI 337 CATTGC GCAATG(2/0) BsrGI 641 TGTACA T^GTACA BssSI 490 CACGAG CACGAG(-5/-1) EcoNI 1157 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 416 TGATCA T^GATCA FseI 1791 GGCCGGCC GGCCGG^CC Hin1I 79 GGCGCC G[GA]^CG[CT]C KasI 79 GGCGCC G^GCGCC NarI 79 GGCGCC GG^CGCC NspI 1192 GCATGT [GA]CATG^[CT] RcaI 1986 TCATGA T^CATGA SfiI 1791 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 79 GGCGCC GGC^GCC SgrAI 887 CGCCGGCG C[GA]^CCGG[CT]G SmlI 235 CTTGAG C^T[CT][GA]AG SspBI 641 TGTACA T^GTACA StuI 1256 AGGCCT AGG^CCT TaqI 181 TCGA T^CGA TthHB8I 181 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 630 GTAC GT^AC 642 GTAC AlwNI 1557 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 1946 CAGCCACTG Aor51HI 1056 AGCGCT AGC^GCT 1592 AGCGCT ApaI 1001 GGGCCC GGGCC^C 1938 GGGCCC Asp700I 903 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1572 GAATGCCTTC BbvCI 423 CCTCAGC CCTCAGC(-5/-2) 1761 CCTCAGC BfaI 1435 CTAG C^TAG 1538 CTAG BglI 205 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1792 GCCGGCCTGGC BsmI 432 GAATGC GAATGC(1/-1) 1572 GAATGC BspMI 394 ACCTGC ACCTGC(4/8) 1493 ACCTGC BssHII 613 GCGCGC G^CGCGC 819 GCGCGC BstAPI 1404 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1556 GCAGGCGCTGC BstXI 722 CCAACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1928 CCATGGAGTTGG Eco47III 1056 AGCGCT AGC^GCT 1592 AGCGCT MaeI 1435 CTAG C^TAG 1538 CTAG MaeII 728 ACGT A^CGT 1133 ACGT MslI 756 CATTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1432 CACCTAGATG NcoI 1073 CCATGG C^CATGG 1928 CCATGG RsaI 630 GTAC GT^AC 642 GTAC SacI 20 GAGCTC GAGCT^C 226 GAGCTC SmaI 464 CCCGGG CCC^GGG 996 CCCGGG XcmI 540 CCACAGGGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1478 CCAGTGCCCAGCTGG XmaCI 464 CCCGGG C^CCGGG 996 CCCGGG XmaI 464 CCCGGG C^CCGGG 996 CCCGGG XmnI 903 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1572 GAATGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI Van91I XhoII