(Circular) (Six-base) MAPSORT of: pFN21AE5750.seq Check: 7440 from: 1 to: 6781 pFN21AE5750 6781 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2408 2408 Size: 6781 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4591 278 Size: 53 83 186 936 3055 2468 Fragments arranged by size: 3055 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4124 1937 Size: 2187 4594 Acc65I G'GTAC_C Cuts at: 4102 4102 Size: 6781 AclI AA'CG_TT Cuts at: 1563 4908 5281 1563 Size: 3345 373 3063 Fragments arranged by size: 3345 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2241 3068 3578 4844 6228 6496 1632 Size: 609 827 510 1266 1384 268 1917 Fragments arranged by size: 1917 1384 1266 827 609 510 268 AfeI AGC'GCT Cuts at: 3063 3599 3063 Size: 536 6245 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6584 AflIII A'CryG_T Cuts at: 1203 5680 6657 1203 Size: 4477 977 1327 Fragments arranged by size: 4477 1327 977 AgeI A'CCGG_T Cuts at: 5612 5612 Size: 6781 AhdI GACnn_n'nnGTC Cuts at: 5510 5510 Size: 6781 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6781 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6781 AlwNI CAG_nnn'CTG Cuts at: 3567 3956 4524 6096 3567 Size: 389 568 1572 4252 Fragments arranged by size: 4252 1572 568 389 ApaI G_GGCC'C Cuts at: 3010 3947 3010 Size: 937 5844 ApaLI G'TGCA_C Cuts at: 4838 5994 4838 Size: 1156 5625 ApoI r'AATT_y Cuts at: 1493 4090 4316 4468 6577 1493 Size: 2597 226 152 2109 1697 Fragments arranged by size: 2597 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5335 160 Size: 872 4303 1606 Fragments arranged by size: 4303 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 AvaI C'yCGr_G Cuts at: 1870 1943 2028 2469 3001 3257 4106 1870 Size: 73 85 441 532 256 849 4545 Fragments arranged by size: 4545 849 532 441 256 85 73 BamHI G'GATC_C Cuts at: 2551 4111 4478 2551 Size: 1560 367 4854 Fragments arranged by size: 4854 1560 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2682 2887 3113 3415 Size: 325 217 627 297 598 205 226 302 Cuts at: 3415 3527 4102 5557 618 Size: 112 575 1455 1842 Fragments arranged by size: 1842 1455 627 598 575 325 302 297 226 217 205 112 BanII G_rGCy'C Cuts at: 1391 2029 2235 2477 3010 3673 3835 3947 3999 Size: 638 206 242 533 663 162 112 52 Cuts at: 3999 4100 1391 Size: 101 4072 Fragments arranged by size: 4072 663 638 533 242 206 162 112 101 52 BbeI G_GCGC'C Cuts at: 2088 2088 Size: 6781 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5810 BbvCI CC'TCA_GC Cuts at: 2429 3767 2429 Size: 1338 5443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4972 5006 1903 Size: 34 3035 34 3678 Fragments arranged by size: 3678 3035 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2270 4673 5883 2270 Size: 2403 1210 3168 Fragments arranged by size: 3168 2403 1210 BclI T'GATC_A Cuts at: 1548 2421 1548 Size: 873 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2216 3803 5392 Size: 107 122 71 631 605 544 1587 1589 Cuts at: 5392 136 Size: 1525 Fragments arranged by size: 1589 1587 1525 631 605 544 122 107 71 BglII A'GATC_T Cuts at: 2499 6776 2499 Size: 4277 2504 BlpI GC'TnA_GC Cuts at: 4198 4198 Size: 6781 Bme1580I G_kGCm'C Cuts at: 1790 3010 3116 3490 3947 4842 5998 1790 Size: 1220 106 374 457 895 1156 2573 Fragments arranged by size: 2573 1220 1156 895 457 374 106 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6781 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2143 3004 3477 Size: 416 53 83 399 24 726 861 473 Cuts at: 3477 3526 4512 5470 442 Size: 49 986 958 1753 Fragments arranged by size: 1753 986 958 861 726 473 416 399 83 53 49 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6781 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2200 2200 Size: 6781 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 2192 2234 2267 2804 3078 3230 3645 Size: 793 160 42 33 537 274 152 415 Cuts at: 3645 5441 1239 Size: 1796 2579 Fragments arranged by size: 2579 1796 793 537 415 274 160 152 42 33 Bpu10I CC'TnA_GC Cuts at: 2429 3767 2429 Size: 1338 5443 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2261 4906 5771 6069 2261 Size: 2645 865 298 2973 Fragments arranged by size: 2973 2645 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2188 5444 882 Size: 617 689 3256 2219 Fragments arranged by size: 3256 2219 689 617 BsaAI yAC'GTr Cuts at: 493 1204 2734 4572 493 Size: 711 1530 1838 2702 Fragments arranged by size: 2702 1838 1530 711 BsaBI GATnn'nnATC Cuts at: 6775 6775 Size: 6781 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 4588 4970 5605 Size: 53 83 186 936 552 2503 382 635 Cuts at: 5605 275 Size: 1451 Fragments arranged by size: 2503 1451 936 635 552 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5214 5612 5886 6033 6444 5214 Size: 398 274 147 411 5551 Fragments arranged by size: 5551 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2697 2727 3203 3233 1178 Size: 30 165 30 1294 30 476 30 4726 Fragments arranged by size: 4726 1294 476 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 2945 3109 3755 1186 Size: 1003 756 164 646 4212 Fragments arranged by size: 4212 1003 756 646 164 BseYI C'CCAG_C Cuts at: 3186 3250 3392 3489 3666 3852 3998 5984 6480 Size: 64 142 97 177 186 146 1986 496 Cuts at: 6480 3186 Size: 3487 Fragments arranged by size: 3487 1986 496 186 177 146 142 97 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2380 3363 3746 3898 1163 Size: 573 644 983 383 152 4046 Fragments arranged by size: 4046 983 644 573 383 152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4231 4992 6020 1540 Size: 275 186 2230 761 1028 2301 Fragments arranged by size: 2301 2230 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2029 2235 3661 4842 4927 5998 2029 Size: 206 1426 1181 85 1071 2812 Fragments arranged by size: 2812 1426 1181 1071 206 85 BsmI GAATG_Cn' Cuts at: 2443 3583 4292 4385 2443 Size: 1140 709 93 4839 Fragments arranged by size: 4839 1140 709 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2717 2717 Size: 6781 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2235 2477 3010 3116 3490 3661 Size: 399 239 206 242 533 106 374 171 Cuts at: 3661 3673 3835 3947 3999 4100 4842 4927 5998 Size: 12 162 112 52 101 742 85 1071 Cuts at: 5998 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 533 399 374 242 239 206 171 162 112 106 101 85 52 12 BspEI T'CCGG_A Cuts at: 6444 6444 Size: 6781 BspHI T'CATG_A Cuts at: 3991 4670 6400 3991 Size: 679 1730 4372 Fragments arranged by size: 4372 1730 679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2408 3507 4136 844 Size: 1564 1099 629 3489 Fragments arranged by size: 3489 1564 1099 629 BsrBI CCG'CTC Cuts at: 1394 1823 2800 4668 1394 Size: 429 977 1868 3507 Fragments arranged by size: 3507 1868 977 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2341 5276 5450 65 Size: 1183 1093 2935 174 1396 Fragments arranged by size: 2935 1396 1183 1093 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2179 2777 2893 3052 3797 5425 5612 Size: 110 369 598 116 159 745 1628 187 Cuts at: 5612 1700 Size: 2869 Fragments arranged by size: 2869 1628 745 598 369 187 159 116 110 BsrGI T'GTAC_A Cuts at: 96 2646 96 Size: 2550 4231 BssHII G'CGCG_C Cuts at: 1925 2618 2824 1925 Size: 693 206 5882 Fragments arranged by size: 5882 693 206 BssSI C'ACGA_G Cuts at: 2495 4841 5853 2495 Size: 2346 1012 3423 Fragments arranged by size: 3423 2346 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3415 3567 794 Size: 2621 152 4008 Fragments arranged by size: 4008 2621 152 BstBI TT'CG_AA Cuts at: 5585 5585 Size: 6781 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6781 BstXI CCAn_nnnn'nTGG Cuts at: 2734 3940 2734 Size: 1206 5575 BstYI r'GATC_y Cuts at: 1972 2499 2551 4067 4111 4478 4863 4880 6332 Size: 527 52 1516 44 367 385 17 1452 Cuts at: 6332 6418 6430 6776 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1516 1452 527 385 367 346 86 52 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3019 3078 3622 3933 513 Size: 552 939 1015 59 544 311 3361 Fragments arranged by size: 3361 1015 939 552 544 311 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3859 487 Size: 683 56 2633 3409 Fragments arranged by size: 3409 2633 683 56 BtsI GCAGTG_nn' Cuts at: 3181 3224 3380 3886 4297 5091 5111 3181 Size: 43 156 506 411 794 20 4851 Fragments arranged by size: 4851 794 506 411 156 43 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6781 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3611 3646 529 Size: 35 3047 35 3664 Fragments arranged by size: 3664 3047 35 35 DraI TTT'AAA Cuts at: 4085 4085 Size: 6781 DraIII CAC_nnn'GTG Cuts at: 3164 3415 3534 3164 Size: 251 119 6411 Fragments arranged by size: 6411 251 119 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5788 809 Size: 507 4472 1802 Fragments arranged by size: 4472 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2072 2462 2542 Size: 54 1425 50 275 18 242 390 80 Cuts at: 2542 2950 3310 3445 3756 4228 5117 6746 8 Size: 408 360 135 311 472 889 1629 43 Fragments arranged by size: 1629 1425 889 472 408 390 360 311 275 242 135 80 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4228 1537 Size: 275 2416 4090 Fragments arranged by size: 4090 2416 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4711 1353 Size: 3358 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5368 5742 5888 387 Size: 1064 138 3779 374 146 1280 Fragments arranged by size: 3779 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2027 2233 2027 Size: 206 6575 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2192 2234 2241 2267 2804 3068 Size: 393 400 160 42 7 26 537 264 Cuts at: 3068 3078 3230 3578 3645 4844 5441 6228 6496 Size: 10 152 348 67 1199 597 787 268 Cuts at: 6496 1239 Size: 1524 Fragments arranged by size: 1524 1199 787 597 537 400 393 348 268 264 160 152 67 42 26 10 7 EcoNI CCTnn'n_nnAGG Cuts at: 3166 3166 Size: 6781 EcoO109I rG'GnC_Cy Cuts at: 2016 2034 2137 2625 3006 3236 3868 3944 2016 Size: 18 103 488 381 230 632 76 4853 Fragments arranged by size: 4853 632 488 381 230 103 76 18 EcoRI G'AATT_C Cuts at: 4090 4468 4090 Size: 378 6403 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3598 3712 3598 Size: 114 6667 FseI GG_CCGG'CC Cuts at: 3801 3801 Size: 6781 FspI TGC'GCA Cuts at: 5287 5287 Size: 6781 HaeII r_GCGC'y Cuts at: 1678 2088 2774 2853 2900 3065 3568 3601 5928 Size: 410 686 79 47 165 503 33 2327 Cuts at: 5928 1678 Size: 2531 Fragments arranged by size: 2531 2327 686 503 410 165 79 47 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4059 4091 5422 5454 5496 Size: 32 135 32 2487 32 1331 32 42 Cuts at: 5496 5528 1373 Size: 32 2626 Fragments arranged by size: 2626 2487 1331 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4125 4371 669 Size: 988 281 2187 246 3079 Fragments arranged by size: 3079 2187 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6781 HpaI GTT'AAC Cuts at: 4371 4371 Size: 6781 KasI G'GCGC_C Cuts at: 2084 2084 Size: 6781 KpnI G_GTAC'C Cuts at: 4106 4106 Size: 6781 MfeI C'AATT_G Cuts at: 4380 4380 Size: 6781 MluI A'CGCG_T Cuts at: 6657 6657 Size: 6781 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5895 6079 1305 Size: 4590 184 2007 Fragments arranged by size: 4590 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2074 2464 2544 2952 3447 Size: 54 1425 343 242 390 80 408 495 Cuts at: 3447 3758 10 Size: 311 3033 Fragments arranged by size: 3033 1425 495 408 390 343 311 242 80 54 MslI CAynn'nnrTG Cuts at: 518 1166 2765 3441 4739 5098 5257 518 Size: 648 1599 676 1298 359 159 2042 Fragments arranged by size: 2042 1599 1298 676 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2518 2821 3493 3852 4197 4874 6022 6267 Size: 912 303 672 359 345 677 1148 245 Cuts at: 6267 1606 Size: 2120 Fragments arranged by size: 2120 1148 912 677 672 359 345 303 245 NaeI GCC'GGC Cuts at: 2895 3054 3799 2895 Size: 159 745 5877 Fragments arranged by size: 5877 745 159 NarI GG'CG_CC Cuts at: 2085 2085 Size: 6781 NcoI C'CATG_G Cuts at: 513 1065 2004 3078 3933 513 Size: 552 939 1074 855 3361 Fragments arranged by size: 3361 1074 939 855 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6781 NgoMIV G'CCGG_C Cuts at: 2893 3052 3797 2893 Size: 159 745 5877 Fragments arranged by size: 5877 745 159 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6781 NotI GC'GGCC_GC Cuts at: 4228 4228 Size: 6781 NspI r_CATG'y Cuts at: 1132 1229 3201 4139 5684 1132 Size: 97 1972 938 1545 2229 Fragments arranged by size: 2229 1972 1545 938 97 PasI CC'CwG_GG Cuts at: 1348 2111 3791 3916 1348 Size: 763 1680 125 4213 Fragments arranged by size: 4213 1680 763 125 PciI A'CATG_T Cuts at: 5680 5680 Size: 6781 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 3079 3698 3916 1108 Size: 902 1069 619 218 3973 Fragments arranged by size: 3973 1069 902 619 218 PfoI T'CCnGG_A Cuts at: 2139 2715 3585 2139 Size: 576 870 5335 Fragments arranged by size: 5335 870 576 PmeI GTTT'AAAC Cuts at: 4085 4085 Size: 6781 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4850 4882 6363 6395 4850 Size: 32 1481 32 5236 Fragments arranged by size: 5236 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2034 2137 2625 3868 2034 Size: 103 488 1243 4947 Fragments arranged by size: 4947 1243 488 103 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6781 PsiI TTA'TAA Cuts at: 4351 4351 Size: 6781 PspOMI G'GGCC_C Cuts at: 3006 3943 3006 Size: 937 5844 PstI C_TGCA'G Cuts at: 830 3375 3572 3843 3863 4133 830 Size: 2545 197 271 20 270 3478 Fragments arranged by size: 3478 2545 271 270 197 20 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6781 PvuII CAG'CTG Cuts at: 2518 2821 3493 3852 2518 Size: 303 672 359 5447 Fragments arranged by size: 5447 672 359 303 SacI G_AGCT'C Cuts at: 2029 2235 2029 Size: 206 6575 SalI G'TCGA_C Cuts at: 1936 4123 1936 Size: 2187 4594 SanDI GG'GwC_CC Cuts at: 2034 3868 2034 Size: 1834 4947 SbfI CC_TGCA'GG Cuts at: 4133 4133 Size: 6781 ScaI AGT'ACT Cuts at: 5029 6741 5029 Size: 1712 5069 SfcI C'TryA_G Cuts at: 826 1045 3371 3568 3839 3859 4129 5264 5945 Size: 219 2326 197 271 20 270 1135 681 Cuts at: 5945 6136 826 Size: 191 1471 Fragments arranged by size: 2326 1471 1135 681 271 270 219 197 191 20 SfiI GGCCn_nnn'nGGCC Cuts at: 3803 3803 Size: 6781 SfoI GGC'GCC Cuts at: 2086 2086 Size: 6781 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 SgrAI Cr'CCGG_yG Cuts at: 1700 2893 1700 Size: 1193 5588 SmaI CCC'GGG Cuts at: 1872 2471 3003 4108 1872 Size: 599 532 1105 4545 Fragments arranged by size: 4545 1105 599 532 SmlI C'TyrA_G Cuts at: 820 1017 1943 2240 4885 5786 6048 820 Size: 197 926 297 2645 901 262 1553 Fragments arranged by size: 2645 1553 926 901 297 262 197 SnaBI TAC'GTA Cuts at: 493 4572 493 Size: 4079 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6781 SphI G_CATG'C Cuts at: 1132 4139 1132 Size: 3007 3774 SspI AAT'ATT Cuts at: 5 52 4705 5 Size: 47 4653 2081 Fragments arranged by size: 4653 2081 47 StuI AGG'CCT Cuts at: 1517 3263 1517 Size: 1746 5035 StyI C'CwwG_G Cuts at: 513 1065 2004 2353 2478 3078 3471 3933 4220 Size: 552 939 349 125 600 393 462 287 Cuts at: 4220 513 Size: 3074 Fragments arranged by size: 3074 939 600 552 462 393 349 287 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2810 3071 3264 3545 4808 4825 4978 Size: 1255 1230 261 193 281 1263 17 153 Cuts at: 4978 5163 5608 325 Size: 185 445 1498 Fragments arranged by size: 1498 1263 1255 1230 445 281 261 193 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2646 5027 6739 Size: 274 80 33 51 1443 669 2381 1712 Cuts at: 6739 96 Size: 138 Fragments arranged by size: 2381 1712 1443 669 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2871 2903 3203 3235 3344 3376 3860 3892 6741 Size: 32 300 32 109 32 484 32 2849 Cuts at: 6741 6773 2871 Size: 32 2879 Fragments arranged by size: 2879 2849 484 300 109 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6781 XbaI T'CTAG_A Cuts at: 4117 4117 Size: 6781 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2552 3490 2552 Size: 938 5843 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6781 XmaI C'CCGG_G Cuts at: 1870 2469 3001 4106 1870 Size: 599 532 1105 4545 Fragments arranged by size: 4545 1105 599 532 XmnI GAAnn'nnTTC Cuts at: 2912 3581 4910 2912 Size: 669 1329 4783 Fragments arranged by size: 4783 1329 669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4589 276 Size: 53 83 186 936 3055 2468 Fragments arranged by size: 3055 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SrfI SwaI