(Linear) (Six-base) MAPSORT of: KIEE5750.seq Check: 648 from: 1 to: 2076 KIEE5750.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 403 2076 Size: 403 1673 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 1063 1573 2076 Size: 236 827 510 503 Fragments arranged by size: 827 510 503 236 AfeI AGC'GCT Cuts at: 0 1058 1594 2076 Size: 1058 536 482 Fragments arranged by size: 1058 536 482 AlwNI CAG_nnn'CTG Cuts at: 0 1562 1951 2076 Size: 1562 389 125 Fragments arranged by size: 1562 389 125 ApaI G_GGCC'C Cuts at: 0 1005 1942 2076 Size: 1005 937 134 Fragments arranged by size: 1005 937 134 AvaI C'yCGr_G Cuts at: 0 23 464 996 1252 2076 Size: 23 441 532 256 824 Fragments arranged by size: 824 532 441 256 23 BamHI G'GATC_C Cuts at: 0 546 2076 Size: 546 1530 BanI G'GyrC_C Cuts at: 0 79 677 882 1108 1410 1522 2076 Size: 79 598 205 226 302 112 554 Fragments arranged by size: 598 554 302 226 205 112 79 BanII G_rGCy'C Cuts at: 0 24 230 472 1005 1668 1830 1942 1994 Size: 24 206 242 533 663 162 112 52 Cuts at: 1994 2076 Size: 82 Fragments arranged by size: 663 533 242 206 162 112 82 52 24 BbeI G_GCGC'C Cuts at: 0 83 2076 Size: 83 1993 BbvCI CC'TCA_GC Cuts at: 0 424 1762 2076 Size: 424 1338 314 Fragments arranged by size: 1338 424 314 BciVI GTATCCnnnnn_n' Cuts at: 0 265 2076 Size: 265 1811 BclI T'GATC_A Cuts at: 0 416 2076 Size: 416 1660 BglI GCCn_nnn'nGGC Cuts at: 0 211 1798 2076 Size: 211 1587 278 Fragments arranged by size: 1587 278 211 BglII A'GATC_T Cuts at: 0 494 2076 Size: 494 1582 Bme1580I G_kGCm'C Cuts at: 0 1005 1111 1485 1942 2076 Size: 1005 106 374 457 134 Fragments arranged by size: 1005 457 374 134 106 BmrI ACTGGGnnnn_n' Cuts at: 0 138 999 1472 1521 2076 Size: 138 861 473 49 555 Fragments arranged by size: 861 555 473 138 49 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 195 2076 Size: 195 1881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 187 229 262 799 1073 1225 1640 Size: 27 160 42 33 537 274 152 415 Cuts at: 1640 2076 Size: 436 Fragments arranged by size: 537 436 415 274 160 152 42 33 27 Bpu10I CC'TnA_GC Cuts at: 0 424 1762 2076 Size: 424 1338 314 Fragments arranged by size: 1338 424 314 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 2076 Size: 256 1820 BsaI GGTCTCn'nnnn_ Cuts at: 0 183 2076 Size: 183 1893 BsaAI yAC'GTr Cuts at: 0 729 2076 Size: 729 1347 BsaHI Gr'CG_yC Cuts at: 0 80 2076 Size: 80 1996 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 692 722 1198 1228 2076 Size: 692 30 476 30 848 Fragments arranged by size: 848 692 476 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 184 940 1104 1750 2076 Size: 184 756 164 646 326 Fragments arranged by size: 756 646 326 184 164 BseYI C'CCAG_C Cuts at: 0 1181 1245 1387 1484 1661 1847 1993 2076 Size: 1181 64 142 97 177 186 146 83 Fragments arranged by size: 1181 186 177 146 142 97 83 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 375 1358 1741 1893 2076 Size: 375 983 383 152 183 Fragments arranged by size: 983 383 375 183 152 BsiHKAI G_wGCw'C Cuts at: 0 24 230 1656 2076 Size: 24 206 1426 420 Fragments arranged by size: 1426 420 206 24 BsmI GAATG_Cn' Cuts at: 0 438 1578 2076 Size: 438 1140 498 Fragments arranged by size: 1140 498 438 BsmBI CGTCTCn'nnnn_ Cuts at: 0 712 2076 Size: 712 1364 Bsp1286I G_dGCh'C Cuts at: 0 24 230 472 1005 1111 1485 1656 1668 Size: 24 206 242 533 106 374 171 12 Cuts at: 1668 1830 1942 1994 2076 Size: 162 112 52 82 Fragments arranged by size: 533 374 242 206 171 162 112 106 82 52 24 12 BspHI T'CATG_A Cuts at: 0 1986 2076 Size: 1986 90 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 403 1502 2076 Size: 403 1099 574 Fragments arranged by size: 1099 574 403 BsrBI CCG'CTC Cuts at: 0 795 2076 Size: 795 1281 BsrDI GCAATG_nn' Cuts at: 0 336 2076 Size: 336 1740 BsrFI r'CCGG_y Cuts at: 0 174 772 888 1047 1792 2076 Size: 174 598 116 159 745 284 Fragments arranged by size: 745 598 284 174 159 116 BsrGI T'GTAC_A Cuts at: 0 641 2076 Size: 641 1435 BssHII G'CGCG_C Cuts at: 0 613 819 2076 Size: 613 206 1257 Fragments arranged by size: 1257 613 206 BssSI C'ACGA_G Cuts at: 0 490 2076 Size: 490 1586 BstAPI GCAn_nnn'nTGC Cuts at: 0 1410 1562 2076 Size: 1410 152 514 Fragments arranged by size: 1410 514 152 BstXI CCAn_nnnn'nTGG Cuts at: 0 729 1935 2076 Size: 729 1206 141 Fragments arranged by size: 1206 729 141 BstYI r'GATC_y Cuts at: 0 494 546 2062 2076 Size: 494 52 1516 14 Fragments arranged by size: 1516 494 52 14 BtgI C'CryG_G Cuts at: 0 1014 1073 1617 1928 2076 Size: 1014 59 544 311 148 Fragments arranged by size: 1014 544 311 148 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1854 2076 Size: 1854 222 BtsI GCAGTG_nn' Cuts at: 0 1176 1219 1375 1881 2076 Size: 1176 43 156 506 195 Fragments arranged by size: 1176 506 195 156 43 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1606 1641 2076 Size: 1606 35 435 Fragments arranged by size: 1606 435 35 DraIII CAC_nnn'GTG Cuts at: 0 1159 1410 1529 2076 Size: 1159 251 119 547 Fragments arranged by size: 1159 547 251 119 EaeI y'GGCC_r Cuts at: 0 67 457 537 945 1305 1440 1751 2076 Size: 67 390 80 408 360 135 311 325 Fragments arranged by size: 408 390 360 325 311 135 80 67 EcoICRI GAG'CTC Cuts at: 0 22 228 2076 Size: 22 206 1848 Fragments arranged by size: 1848 206 22 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 187 229 236 262 799 1063 1073 Size: 27 160 42 7 26 537 264 10 Cuts at: 1073 1225 1573 1640 2076 Size: 152 348 67 436 Fragments arranged by size: 537 436 348 264 160 152 67 42 27 26 10 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 1161 2076 Size: 1161 915 EcoO109I rG'GnC_Cy Cuts at: 0 11 29 132 620 1001 1231 1863 1939 Size: 11 18 103 488 381 230 632 76 Cuts at: 1939 2076 Size: 137 Fragments arranged by size: 632 488 381 230 137 103 76 18 11 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1593 1707 2076 Size: 1593 114 369 Fragments arranged by size: 1593 369 114 FseI GG_CCGG'CC Cuts at: 0 1796 2076 Size: 1796 280 HaeII r_GCGC'y Cuts at: 0 83 769 848 895 1060 1563 1596 2076 Size: 83 686 79 47 165 503 33 480 Fragments arranged by size: 686 503 480 165 83 79 47 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2054 2076 Size: 2054 22 KasI G'GCGC_C Cuts at: 0 79 2076 Size: 79 1997 MscI TGG'CCA Cuts at: 0 69 459 539 947 1442 1753 2076 Size: 69 390 80 408 495 311 323 Fragments arranged by size: 495 408 390 323 311 80 69 MslI CAynn'nnrTG Cuts at: 0 760 1436 2076 Size: 760 676 640 Fragments arranged by size: 760 676 640 MspA1I CmG'CkG Cuts at: 0 513 816 1488 1847 2076 Size: 513 303 672 359 229 Fragments arranged by size: 672 513 359 303 229 NaeI GCC'GGC Cuts at: 0 890 1049 1794 2076 Size: 890 159 745 282 Fragments arranged by size: 890 745 282 159 NarI GG'CG_CC Cuts at: 0 80 2076 Size: 80 1996 NcoI C'CATG_G Cuts at: 0 1073 1928 2076 Size: 1073 855 148 Fragments arranged by size: 1073 855 148 NgoMIV G'CCGG_C Cuts at: 0 888 1047 1792 2076 Size: 888 159 745 284 Fragments arranged by size: 888 745 284 159 NspI r_CATG'y Cuts at: 0 1196 2076 Size: 1196 880 PasI CC'CwG_GG Cuts at: 0 106 1786 1911 2076 Size: 106 1680 125 165 Fragments arranged by size: 1680 165 125 106 PflMI CCAn_nnn'nTGG Cuts at: 0 1074 1693 1911 2076 Size: 1074 619 218 165 Fragments arranged by size: 1074 619 218 165 PfoI T'CCnGG_A Cuts at: 0 134 710 1580 2076 Size: 134 576 870 496 Fragments arranged by size: 870 576 496 134 PpuMI rG'GwC_Cy Cuts at: 0 29 132 620 1863 2076 Size: 29 103 488 1243 213 Fragments arranged by size: 1243 488 213 103 29 PspOMI G'GGCC_C Cuts at: 0 1001 1938 2076 Size: 1001 937 138 Fragments arranged by size: 1001 937 138 PstI C_TGCA'G Cuts at: 0 1370 1567 1838 1858 2076 Size: 1370 197 271 20 218 Fragments arranged by size: 1370 271 218 197 20 PvuII CAG'CTG Cuts at: 0 513 816 1488 1847 2076 Size: 513 303 672 359 229 Fragments arranged by size: 672 513 359 303 229 SacI G_AGCT'C Cuts at: 0 24 230 2076 Size: 24 206 1846 Fragments arranged by size: 1846 206 24 SanDI GG'GwC_CC Cuts at: 0 29 1863 2076 Size: 29 1834 213 Fragments arranged by size: 1834 213 29 SfcI C'TryA_G Cuts at: 0 1366 1563 1834 1854 2076 Size: 1366 197 271 20 222 Fragments arranged by size: 1366 271 222 197 20 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1798 2076 Size: 1798 278 SfoI GGC'GCC Cuts at: 0 81 2076 Size: 81 1995 SgrAI Cr'CCGG_yG Cuts at: 0 888 2076 Size: 888 1188 SmaI CCC'GGG Cuts at: 0 466 998 2076 Size: 466 532 1078 Fragments arranged by size: 1078 532 466 SmlI C'TyrA_G Cuts at: 0 235 2076 Size: 235 1841 StuI AGG'CCT Cuts at: 0 1258 2076 Size: 1258 818 StyI C'CwwG_G Cuts at: 0 348 473 1073 1466 1928 2076 Size: 348 125 600 393 462 148 Fragments arranged by size: 600 462 393 348 148 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 805 1066 1259 1540 2076 Size: 805 261 193 281 536 Fragments arranged by size: 805 536 281 261 193 TatI w'GTAC_w Cuts at: 0 641 2076 Size: 641 1435 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 866 898 1198 1230 1339 1371 1855 1887 Size: 866 32 300 32 109 32 484 32 Cuts at: 1887 2076 Size: 189 Fragments arranged by size: 866 484 300 189 109 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 547 1485 2076 Size: 547 938 591 Fragments arranged by size: 938 591 547 XmaI C'CCGG_G Cuts at: 0 464 996 2076 Size: 464 532 1080 Fragments arranged by size: 1080 532 464 XmnI GAAnn'nnTTC Cuts at: 0 907 1576 2076 Size: 907 669 500 Fragments arranged by size: 907 669 500 Enzymes that do cut: AarI AcuI AfeI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI BfrBI BlpI BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BspEI BstBI BstEII BstZ17I Bsu36I ClaI DraI DrdI EagI EarI EciI EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI