################################## # NO CUT ENZYMES ( 230 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI ItaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 19 enzymes) # ################################### AhdI 146 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 174 AGCT AG^CT AlwI 231 GATCC GGATC(4/5) AlwNI 173 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 146 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BsmFI 144 GGGAC GGGAC(10/14) BsrDI 76 GCAATG GCAATG(2/0) BstXI 65 CCACTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 68 CTGAG C^T[ACGT]AG DpnII 231 GATC ^GATC Eam1105I 146 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 93 CTCTTC CTCTTC(1/4) Ksp632I 93 CTCTTC CTCTTC(1/4) MboI 231 GATC ^GATC MslI 66 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 231 GATC ^GATC Sau3AI 231 GATC ^GATC SfcI 179 CTGTAG C^T[GA][CT]AG Tsp45I 103 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AfaI 62 GTAC GT^AC 169 GTAC MaeIII 103 GTCAC ^GT[ACGT]AC 113 GTTAC MboII 94 TCTTC GAAGA(8/7) 97 TCTTC MseI 58 TTAA T^TAA 238 TTAA RsaI 62 GTAC GT^AC 169 GTAC Tru9I 58 TTAA T^TAA 238 TTAA Tsp509I 39 AATT ^AATT 240 AATT TspRI 66 CACTG CA[GC]TG[ACGT][ACGT]^ 165 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### MnlI