################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AccI AccII AccIII AciI AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV FauI FseI FspI HaeII HaeIII HgaI HhaI HinP1I HindIII KasI KspI MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 630 GACGTC GACGT^C Acc65I 476 GGTACC G^GTACC AcyI 630 GACGTC G[GA]^CG[CT]C AluI 379 AGCT AG^CT AocI 430 CCTCAGG CC^T[ACGT]AGG AseI 408 ATTAAT AT^TAAT AsnI 408 ATTAAT AT^TAAT Asp718I 476 GGTACC G^GTACC AspHI 85 GAGCAC G[AT]GC[AT]^C AvaII 483 GGACC G^G[AT]CC BamHI 856 GGATCC G^GATCC BanII 230 GGGCTC G[GA]GC[CT]^C BbvI 396 GCAGC GCAGC(8/12) BclI 168 TGATCA T^GATCA BglII 750 AGATCT A^GATCT BsaHI 630 GACGTC G[GA]^CG[CT]C BseRI 381 CTCCTC GAGGAG(10/8) BsiHKAI 85 GAGCAC G[AT]GC[AT]^C BsmBI 632 CGTCTC CGTCTC(1/5) BssSI 835 CTCGTG CACGAG(-5/-1) BstAPI 443 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 333 GGTGACC G^GT[ACGT]ACC BstF5I 711 CATCC GGATG(2/0) BstPI 333 GGTGACC G^GT[ACGT]ACC BstXI 140 CCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 430 CCTCAGG CC^T[ACGT]AGG Cac8I 135 GCAAGC GC[ACGT]^[ACGT]GC Cfr13I 483 GGACC G^G[ACGT]CC DraI 763 TTTAAA TTT^AAA DsaI 140 CCATGG C^C[GA][CT]GG Eco81I 430 CCTCAGG CC^T[ACGT]AGG EcoO65I 333 GGTGACC G^GT[ACGT]ACC EcoT22I 124 ATGCAT ATGCA^T FbaI 168 TGATCA T^GATCA Fnu4HI 396 GCAGC GC^[ACGT]GC FokI 711 CATCC GGATG(9/13) Hin1I 630 GACGTC G[GA]^CG[CT]C HincII 292 GTTAAC GT[CT]^[GA]AC HindII 292 GTTAAC GT[CT]^[GA]AC HpaI 292 GTTAAC GTT^AAC ItaI 396 GCAGC GC^[ACGT]GC KpnI 476 GGTACC GGTAC^C MaeII 631 ACGT A^CGT MaeIII 334 GTGAC ^GT[ACGT]AC MfeI 593 CAATTG C^AATTG MslI 306 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 593 CAATTG C^AATTG NcoI 140 CCATGG C^CATGG NsiI 124 ATGCAT ATGCA^T NspI 122 ACATGC [GA]CATG^[CT] PleI 607 GAGTC GAGTC(4/5) PshBI 408 ATTAAT AT^TAAT Sau96I 483 GGACC G^G[ACGT]CC SbfI 327 CCTGCAGG CCTGCA^GG SexAI 846 ACCTGGT A^CC[AT]GGT SfaNI 364 GATGC GCATC(5/9) SmlI 82 CTTGAG C^T[CT][GA]AG Sse8387I 327 CCTGCAGG CCTGCA^GG TfiI 470 GAATC G^A[AT]TC TseI 396 GCAGC G^C[AT]GC Tsp45I 334 GTGAC ^GT[GC]AC XbaI 402 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 520 GAATTT [GA]^AATT[CT] 727 AAATTC AfaI 225 GTAC GT^AC 477 GTAC ApoI 520 GAATTT [GA]^AATT[CT] 727 AAATTC BanI 435 GGTGCC G^G[CT][GA]CC 476 GGTACC BcnI 96 CCGGG CC^[GC]GG 189 CCGGG BfaI 44 CTAG C^TAG 403 CTAG BpmI 205 CTCCAG CTGGAG(16/14) 287 CTCCAG BsmAI 203 GTCTC GTCTC(1/5) 633 GTCTC BspMI 623 ACCTGC ACCTGC(4/8) 330 GCAGGT EarI 460 GAAGAG CTCTTC(1/4) 781 GAAGAG EcoT14I 140 CCATGG C^C[AT][AT]GG 821 CCTTGG HapII 96 CCGG C^CGG 189 CCGG HinfI 470 GAATC G^A[ACGT]TC 607 GAGTC HpaII 96 CCGG C^CGG 189 CCGG Ksp632I 460 GAAGAG CTCTTC(1/4) 781 GAAGAG MaeI 44 CTAG C^TAG 403 CTAG MspI 96 CCGG C^CGG 189 CCGG NciI 96 CCGGG CC^[GC]GG 189 CCGGG PstI 328 CTGCAG CTGCA^G 625 CTGCAG RsaI 225 GTAC GT^AC 477 GTAC StyI 140 CCATGG C^C[AT][AT]GG 821 CCTTGG TaqI 362 TCGA T^CGA 758 TCGA TspRI 306 CACTG CA[GC]TG[ACGT][ACGT]^ 774 CACTG TthHB8I 362 TCGA T^CGA 758 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AlwI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstYI DdeI DpnII EcoRII HphI MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfcI Tru9I Tsp509I XhoII