Restriction map for
pFN21AE2489
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspI AviII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmFI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstF5I BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 959 GTAGAC GT^[AC][GT]AC
AciI 1554 GCGG CCGC(-3/-1)
AhdI 601 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1207 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 760 ATTAAT AT^TAAT
AsnI 760 ATTAAT AT^TAAT
AspEI 601 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1445 CCCGAG C^[CT]CG[GA]G
AvrII 370 CCTAGG C^CTAGG
BalI 1502 TGGCCA TGG^CCA
BanII 1627 GAGCTC G[GA]GC[CT]^C
BbsI 1161 GAAGAC GAAGAC(2/6)
BlnI 370 CCTAGG C^CTAGG
BlpI 1624 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1624 GCTGAGC GC^T[ACGT]AGC
BpuAI 1161 GAAGAC GAAGAC(2/6)
BsaAI 1594 CACGTA [CT]AC^GT[GA]
BsgI 231 CTGCAC GTGCAG(16/14)
BsiYI 1494 CCAGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1494 CCAGGAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 142 CGTCTC CGTCTC(1/5)
BsoBI 1445 CCCGAG C^[CT]CG[GA]G
BspHI 183 TCATGA T^CATGA
BstAPI 413 GCAACTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1489 GGTCACC G^GT[ACGT]ACC
BstPI 1489 GGTCACC G^GT[ACGT]ACC
Cac8I 1406 GCTCGC GC[ACGT]^[ACGT]GC
CelII 1624 GCTGAGC GC^T[ACGT]AGC
DrdI 1193 GACACCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1502 TGGCCA [CT]^GGCC[GA]
Eam1105I 601 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1489 GGTCACC G^GT[ACGT]ACC
HgaI 875 GCGTC GACGC(5/10)
HincII 986 GTCAAC GT[CT]^[GA]AC
HindII 986 GTCAAC GT[CT]^[GA]AC
MaeII 1595 ACGT A^CGT
MluNI 1502 TGGCCA TGG^CCA
MscI 1502 TGGCCA TGG^CCA
MspA1I 1622 CAGCTG C[AC]G^C[GT]G
PflMI 1494 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1208 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 45 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 760 ATTAAT AT^TAAT
PvuII 1622 CAGCTG CAG^CTG
RcaI 183 TCATGA T^CATGA
SacI 1627 GAGCTC GAGCT^C
SapI 1428 GAAGAGC GCTCTTC(1/4)
ScaI 1042 AGTACT AGT^ACT
StuI 118 AGGCCT AGG^CCT
Van91I 1494 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 101 GTAC GT^AC
1043 GTAC
AspHI 1404 GTGCTC G[AT]GC[AT]^C
1627 GAGCTC
AvaII 138 GGTCC G^G[AT]CC
1209 GGACC
BbvI 472 GCAGC GCAGC(8/12)
825 GCAGC
BsiHKAI 1404 GTGCTC G[AT]GC[AT]^C
1627 GAGCTC
BsmI 347 GCATTC GAATGC(1/-1)
828 GCATTC
Fnu4HI 472 GCAGC GC^[ACGT]GC
825 GCAGC
HindIII 743 AAGCTT A^AGCTT
845 AAGCTT
ItaI 472 GCAGC GC^[ACGT]GC
825 GCAGC
MfeI 1057 CAATTG C^AATTG
1534 CAATTG
MunI 1057 CAATTG C^AATTG
1534 CAATTG
PstI 823 CTGCAG CTGCA^G
1112 CTGCAG
RsaI 101 GTAC GT^AC
1043 GTAC
SfaNI 1315 GATGC GCATC(5/9)
1329 GCATC
SfcI 823 CTGCAG C^T[GA][CT]AG
1112 CTGCAG
SmlI 263 CTCAAG C^T[CT][GA]AG
1048 CTTGAG
SspI 169 AATATT AAT^ATT
1323 AATATT
TseI 472 GCAGC G^C[AT]GC
825 GCAGC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AluI AlwI ApoI BfaI
BglII BmyI BpmI BsaJI BseRI
BsmAI Bsp1286I BsrI BssKI BstNI
BstYI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I HaeIII
HinfI HphI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
StyI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I XhoII