(Circular) (Six-base) MAPSORT of: pFN21AE2489.seq Check: 921 from: 1 to: 6445 pFN21AE2489 6445 bp With 182 enzymes: SgfI * November 15, 2013 16:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4255 278 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2965 3788 1937 Size: 1028 823 4594 Fragments arranged by size: 4594 1028 823 Acc65I G'GTAC_C Cuts at: 3766 3766 Size: 6445 AclI AA'CG_TT Cuts at: 1563 4572 4945 1563 Size: 3009 373 3063 Fragments arranged by size: 3063 3009 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2429 2537 2739 4508 5892 6160 1632 Size: 797 108 202 1769 1384 268 1917 Fragments arranged by size: 1917 1769 1384 797 268 202 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 5344 6321 1203 Size: 4141 977 1327 Fragments arranged by size: 4141 1327 977 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 2611 5174 2611 Size: 2563 3882 AleI CACnn'nnGTG Cuts at: 1166 2985 3119 3716 1166 Size: 1819 134 597 3895 Fragments arranged by size: 3895 1819 597 134 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6445 AlwNI CAG_nnn'CTG Cuts at: 3217 4188 5760 3217 Size: 971 1572 3902 Fragments arranged by size: 3902 1572 971 ApaLI G'TGCA_C Cuts at: 4502 5658 4502 Size: 1156 5289 ApoI r'AATT_y Cuts at: 1493 2303 2449 3314 3754 3980 4132 6241 1493 Size: 810 146 865 440 226 152 2109 1697 Fragments arranged by size: 2109 1697 865 810 440 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 2766 4999 160 Size: 872 1734 2233 1606 Fragments arranged by size: 2233 1734 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 3450 3770 1870 Size: 73 1507 320 4545 Fragments arranged by size: 4545 1507 320 73 AvrII C'CTAG_G Cuts at: 2375 2375 Size: 6445 BamHI G'GATC_C Cuts at: 3775 4142 3775 Size: 367 6078 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3766 5221 618 Size: 325 217 627 1979 1455 1842 Fragments arranged by size: 1979 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3636 3764 1391 Size: 2245 128 4072 Fragments arranged by size: 4072 2245 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3173 928 Size: 971 1274 4200 Fragments arranged by size: 4200 1274 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4636 4670 1903 Size: 34 2699 34 3678 Fragments arranged by size: 3678 2699 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4337 5547 4337 Size: 1210 5235 BclI T'GATC_A Cuts at: 1548 1548 Size: 6445 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5056 136 Size: 107 122 71 631 605 3384 1525 Fragments arranged by size: 3384 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2694 2887 3256 6440 2694 Size: 193 369 3184 2699 Fragments arranged by size: 3184 2699 369 193 BlpI GC'TnA_GC Cuts at: 3630 3862 3630 Size: 232 6213 Bme1580I G_kGCm'C Cuts at: 1790 2982 4506 5662 1790 Size: 1192 1524 1156 2573 Fragments arranged by size: 2573 1524 1192 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6445 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2149 3583 4176 Size: 416 53 83 399 24 732 1434 593 Cuts at: 4176 5134 442 Size: 958 1753 Fragments arranged by size: 1753 1434 958 732 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2584 2880 3620 3699 5105 1239 Size: 1345 296 740 79 1406 2579 Fragments arranged by size: 2579 1406 1345 740 296 79 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2253 3074 4570 5435 5733 2253 Size: 821 1496 865 298 2965 Fragments arranged by size: 2965 1496 865 821 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5108 882 Size: 617 3609 2219 Fragments arranged by size: 3609 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3601 4236 493 Size: 711 2397 635 2702 Fragments arranged by size: 2702 2397 711 635 BsaBI GATnn'nnATC Cuts at: 6439 6439 Size: 6445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4252 4634 5269 275 Size: 53 83 186 936 2719 382 635 1451 Fragments arranged by size: 2719 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4878 5276 5550 5697 6108 4878 Size: 398 274 147 411 5215 Fragments arranged by size: 5215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6220 Fragments arranged by size: 6220 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2505 3165 3509 1186 Size: 856 463 660 344 4122 Fragments arranged by size: 4122 856 660 463 344 BseYI C'CCAG_C Cuts at: 5648 6144 5648 Size: 496 5949 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2221 1163 Size: 573 485 5387 Fragments arranged by size: 5387 573 485 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3895 4656 5684 1540 Size: 275 186 1894 761 1028 2301 Fragments arranged by size: 2301 1894 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3413 3636 4506 4591 5662 3413 Size: 223 870 85 1071 4196 Fragments arranged by size: 4196 1071 870 223 85 BsmI GAATG_Cn' Cuts at: 2352 2833 3956 4049 2352 Size: 481 1123 93 4748 Fragments arranged by size: 4748 1123 481 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2153 2153 Size: 6445 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2982 3413 3636 3764 4506 4591 5662 Size: 399 1192 431 223 128 742 85 1071 Cuts at: 5662 1391 Size: 2174 Fragments arranged by size: 2174 1192 1071 742 431 399 223 128 85 BspEI T'CCGG_A Cuts at: 6108 6108 Size: 6445 BspHI T'CATG_A Cuts at: 2188 4334 6064 2188 Size: 2146 1730 2569 Fragments arranged by size: 2569 2146 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3800 844 Size: 2956 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4332 1394 Size: 429 2509 3507 Fragments arranged by size: 3507 2509 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4940 5114 65 Size: 1183 3692 174 1396 Fragments arranged by size: 3692 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5089 5276 1700 Size: 110 3279 187 2869 Fragments arranged by size: 3279 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6445 BssSI C'ACGA_G Cuts at: 4505 5517 4505 Size: 1012 5433 BstAPI GCAn_nnn'nTGC Cuts at: 794 2424 794 Size: 1630 4815 BstBI TT'CG_AA Cuts at: 5249 5249 Size: 6445 BstEII G'GTnAC_C Cuts at: 1184 3494 1184 Size: 2310 4135 BstYI r'GATC_y Cuts at: 1972 2546 2694 2707 2794 2887 3256 3775 4142 Size: 574 148 13 87 93 369 519 367 Cuts at: 4142 4527 4544 5996 6082 6094 6440 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 574 519 385 369 367 346 148 93 87 86 17 13 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4954 Fragments arranged by size: 4954 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5706 Fragments arranged by size: 5706 683 56 BtsI GCAGTG_nn' Cuts at: 3114 3126 3961 4755 4775 3114 Size: 12 835 794 20 4784 Fragments arranged by size: 4784 835 794 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6410 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3204 5452 809 Size: 507 1888 2248 1802 Fragments arranged by size: 2248 1888 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3507 3892 4781 Size: 54 1425 50 275 18 1677 385 889 Cuts at: 4781 6410 8 Size: 1629 43 Fragments arranged by size: 1677 1629 1425 889 385 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3892 1537 Size: 275 2080 4090 Fragments arranged by size: 4090 2080 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2032 2451 3428 4375 1353 Size: 679 419 977 947 3423 Fragments arranged by size: 3423 977 947 679 419 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5032 5406 5552 387 Size: 1064 138 3443 374 146 1280 Fragments arranged by size: 3443 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3634 3634 Size: 6445 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2537 2584 2739 2880 3620 3699 Size: 393 797 108 47 155 141 740 79 Cuts at: 3699 4508 5105 5892 6160 1239 Size: 809 597 787 268 1524 Fragments arranged by size: 1524 809 797 787 740 597 393 268 155 141 108 79 47 EcoO109I rG'GnC_Cy Cuts at: 2396 3214 3444 2396 Size: 818 230 5397 Fragments arranged by size: 5397 818 230 EcoRI G'AATT_C Cuts at: 3754 4132 3754 Size: 378 6067 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2404 2829 2404 Size: 425 6020 FspI TGC'GCA Cuts at: 4951 4951 Size: 6445 HaeII r_GCGC'y Cuts at: 1678 5592 1678 Size: 3914 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2597 2629 2858 2890 3160 Size: 32 135 32 1025 32 229 32 270 Cuts at: 3160 3192 3206 3225 3238 3257 5086 5118 5160 Size: 32 14 19 13 19 1829 32 42 Cuts at: 5160 5192 1373 Size: 32 2626 Fragments arranged by size: 2626 1829 1025 270 229 135 42 32 32 32 32 32 32 32 19 19 14 13 HincII GTy'rAC Cuts at: 669 1657 1938 2993 3789 4035 669 Size: 988 281 1055 796 246 3079 Fragments arranged by size: 3079 1055 988 796 281 246 HindIII A'AGCT_T Cuts at: 748 2748 2850 748 Size: 2000 102 4343 Fragments arranged by size: 4343 2000 102 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KpnI G_GTAC'C Cuts at: 3770 3770 Size: 6445 MfeI C'AATT_G Cuts at: 3062 3539 4044 3062 Size: 477 505 5463 Fragments arranged by size: 5463 505 477 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3050 5559 5743 1305 Size: 1745 2509 184 2007 Fragments arranged by size: 2509 2007 1745 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3509 10 Size: 54 1425 343 1677 2946 Fragments arranged by size: 2946 1677 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2985 3119 3716 4403 4762 4921 518 Size: 648 1819 134 597 687 359 159 2042 Fragments arranged by size: 2042 1819 687 648 597 359 159 134 MspA1I CmG'CkG Cuts at: 1606 3629 3861 4538 5686 5931 1606 Size: 2023 232 677 1148 245 2120 Fragments arranged by size: 2120 2023 1148 677 245 232 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4954 Fragments arranged by size: 4954 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6445 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 3803 5348 1132 Size: 97 2574 1545 2229 Fragments arranged by size: 2574 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6445 PciI A'CATG_T Cuts at: 5344 5344 Size: 6445 PflMI CCAn_nnn'nTGG Cuts at: 1108 3505 1108 Size: 2397 4048 PfoI T'CCnGG_A Cuts at: 3636 3636 Size: 6445 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4514 4546 6027 6059 4514 Size: 32 1481 32 4900 Fragments arranged by size: 4900 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3214 3214 Size: 6445 PshAI GACnn'nnGTC Cuts at: 1613 2054 1613 Size: 441 6004 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PstI C_TGCA'G Cuts at: 830 2832 3121 3797 830 Size: 2002 289 676 3478 Fragments arranged by size: 3478 2002 676 289 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 3629 3629 Size: 6445 SacI G_AGCT'C Cuts at: 3636 3636 Size: 6445 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SapI GCTCTTCn'nnn_ Cuts at: 3428 3428 Size: 6445 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 3049 4693 6405 3049 Size: 1644 1712 3089 Fragments arranged by size: 3089 1712 1644 SfcI C'TryA_G Cuts at: 826 1045 2828 3117 3793 4928 5609 5800 826 Size: 219 1783 289 676 1135 681 191 1471 Fragments arranged by size: 1783 1471 1135 681 676 289 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6445 SmaI CCC'GGG Cuts at: 1872 3772 1872 Size: 1900 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2268 3053 4549 5450 5712 820 Size: 197 926 325 785 1496 901 262 1553 Fragments arranged by size: 1553 1496 926 901 785 325 262 197 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 3803 1132 Size: 2671 3774 SspI AAT'ATT Cuts at: 5 52 2176 3330 4369 5 Size: 47 2124 1154 1039 2081 Fragments arranged by size: 2124 2081 1154 1039 47 StuI AGG'CCT Cuts at: 1517 2125 1517 Size: 608 5837 StyI C'CwwG_G Cuts at: 513 1065 2004 2375 2937 3588 3884 513 Size: 552 939 371 562 651 296 3074 Fragments arranged by size: 3074 939 651 562 552 371 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4472 4489 4642 4827 5272 325 Size: 1255 2892 17 153 185 445 1498 Fragments arranged by size: 2892 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2105 3047 4691 Size: 274 80 33 51 1443 128 942 1644 Cuts at: 4691 6403 96 Size: 1712 138 Fragments arranged by size: 1712 1644 1443 942 274 138 128 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6405 6437 6405 Size: 32 6413 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6445 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6445 XmaI C'CCGG_G Cuts at: 1870 3770 1870 Size: 1900 4545 XmnI GAAnn'nnTTC Cuts at: 4574 4574 Size: 6445 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4253 276 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI