Restriction map for
pFN21AE2489
(Linear) (Six-base) MAPSORT of: KIEE2489.seq Check: 3602 from: 1 to: 1740
KIEE2489.orf
With 182 enzymes: SgfI *
November 15, 2013 14:41 ..
AccI GT'mk_AC
Cuts at: 0 960 1740
Size: 960 780
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 532 734 1740
Size: 424 108 202 1006
Fragments arranged by size:
1006 424 202 108
AhdI GACnn_n'nnGTC
Cuts at: 0 606 1740
Size: 606 1134
AleI CACnn'nnGTG
Cuts at: 0 980 1114 1711 1740
Size: 980 134 597 29
Fragments arranged by size:
980 597 134 29
AlwNI CAG_nnn'CTG
Cuts at: 0 1212 1740
Size: 1212 528
ApoI r'AATT_y
Cuts at: 0 298 444 1309 1740
Size: 298 146 865 431
Fragments arranged by size:
865 431 298 146
AseI AT'TA_AT
Cuts at: 0 761 1740
Size: 761 979
AvaI C'yCGr_G
Cuts at: 0 1445 1740
Size: 1445 295
AvrII C'CTAG_G
Cuts at: 0 370 1740
Size: 370 1370
BanII G_rGCy'C
Cuts at: 0 1631 1740
Size: 1631 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 1168 1740
Size: 1168 572
BglII A'GATC_T
Cuts at: 0 689 882 1251 1740
Size: 689 193 369 489
Fragments arranged by size:
689 489 369 193
BlpI GC'TnA_GC
Cuts at: 0 1625 1740
Size: 1625 115
Bme1580I G_kGCm'C
Cuts at: 0 977 1740
Size: 977 763
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 1578 1740
Size: 144 1434 162
Fragments arranged by size:
1434 162 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 875 1615 1694 1740
Size: 579 296 740 79 46
Fragments arranged by size:
740 579 296 79 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1069 1740
Size: 248 821 671
Fragments arranged by size:
821 671 248
BsaAI yAC'GTr
Cuts at: 0 1596 1740
Size: 1596 144
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 500 1160 1504 1740
Size: 37 463 660 344 236
Fragments arranged by size:
660 463 344 236 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 1740
Size: 216 1524
BsiHKAI G_wGCw'C
Cuts at: 0 1408 1631 1740
Size: 1408 223 109
Fragments arranged by size:
1408 223 109
BsmI GAATG_Cn'
Cuts at: 0 347 828 1740
Size: 347 481 912
Fragments arranged by size:
912 481 347
BsmBI CGTCTCn'nnnn_
Cuts at: 0 148 1740
Size: 148 1592
Bsp1286I G_dGCh'C
Cuts at: 0 977 1408 1631 1740
Size: 977 431 223 109
Fragments arranged by size:
977 431 223 109
BspHI T'CATG_A
Cuts at: 0 183 1740
Size: 183 1557
BstAPI GCAn_nnn'nTGC
Cuts at: 0 419 1740
Size: 419 1321
BstEII G'GTnAC_C
Cuts at: 0 1489 1740
Size: 1489 251
BstYI r'GATC_y
Cuts at: 0 541 689 702 789 882 1251 1740
Size: 541 148 13 87 93 369 489
Fragments arranged by size:
541 489 369 148 93 87 13
BtsI GCAGTG_nn'
Cuts at: 0 1109 1121 1740
Size: 1109 12 619
Fragments arranged by size:
1109 619 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 1199 1740
Size: 1199 541
EaeI y'GGCC_r
Cuts at: 0 1502 1740
Size: 1502 238
EarI CTCTTCn'nnn_
Cuts at: 0 27 446 1423 1740
Size: 27 419 977 317
Fragments arranged by size:
977 419 317 27
EcoICRI GAG'CTC
Cuts at: 0 1629 1740
Size: 1629 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 532 579 734 875 1615 1694 1740
Size: 424 108 47 155 141 740 79 46
Fragments arranged by size:
740 424 155 141 108 79 47 46
EcoO109I rG'GnC_Cy
Cuts at: 0 391 1209 1439 1740
Size: 391 818 230 301
Fragments arranged by size:
818 391 301 230
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 399 824 1740
Size: 399 425 916
Fragments arranged by size:
916 425 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 853 885 1155 1187 1201 1220
Size: 592 32 229 32 270 32 14 19
Cuts at: 1220 1233 1252 1740
Size: 13 19 488
Fragments arranged by size:
592 488 270 229 32 32 32 19
19 14 13
HincII GTy'rAC
Cuts at: 0 988 1740
Size: 988 752
HindIII A'AGCT_T
Cuts at: 0 743 845 1740
Size: 743 102 895
Fragments arranged by size:
895 743 102
MfeI C'AATT_G
Cuts at: 0 1057 1534 1740
Size: 1057 477 206
Fragments arranged by size:
1057 477 206
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1740
Size: 1045 695
MscI TGG'CCA
Cuts at: 0 1504 1740
Size: 1504 236
MslI CAynn'nnrTG
Cuts at: 0 980 1114 1711 1740
Size: 980 134 597 29
Fragments arranged by size:
980 597 134 29
MspA1I CmG'CkG
Cuts at: 0 1624 1740
Size: 1624 116
PflMI CCAn_nnn'nTGG
Cuts at: 0 1500 1740
Size: 1500 240
PfoI T'CCnGG_A
Cuts at: 0 1631 1740
Size: 1631 109
PpuMI rG'GwC_Cy
Cuts at: 0 1209 1740
Size: 1209 531
PshAI GACnn'nnGTC
Cuts at: 0 49 1740
Size: 49 1691
PstI C_TGCA'G
Cuts at: 0 827 1116 1740
Size: 827 289 624
Fragments arranged by size:
827 624 289
PvuII CAG'CTG
Cuts at: 0 1624 1740
Size: 1624 116
SacI G_AGCT'C
Cuts at: 0 1631 1740
Size: 1631 109
SapI GCTCTTCn'nnn_
Cuts at: 0 1423 1740
Size: 1423 317
ScaI AGT'ACT
Cuts at: 0 1044 1740
Size: 1044 696
SfcI C'TryA_G
Cuts at: 0 823 1112 1740
Size: 823 289 628
Fragments arranged by size:
823 628 289
SmlI C'TyrA_G
Cuts at: 0 263 1048 1740
Size: 263 785 692
Fragments arranged by size:
785 692 263
SspI AAT'ATT
Cuts at: 0 171 1325 1740
Size: 171 1154 415
Fragments arranged by size:
1154 415 171
StuI AGG'CCT
Cuts at: 0 120 1740
Size: 120 1620
StyI C'CwwG_G
Cuts at: 0 370 932 1583 1740
Size: 370 562 651 157
Fragments arranged by size:
651 562 370 157
TatI w'GTAC_w
Cuts at: 0 100 1042 1740
Size: 100 942 698
Fragments arranged by size:
942 698 100
Enzymes that do cut:
AccI AcuI AhdI AleI AlwNI ApoI AseI AvaI
AvrII BanII BbsI BglII BlpI Bme1580I BmrI BpmI
BpuEI BsaAI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BstAPI BstEII BstYI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I PflMI PfoI PpuMI PshAI
PstI PvuII SacI SapI ScaI SfcI SmlI SspI
StuI StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI ApaI ApaLI AscI AsiSI BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI