################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrI BlnI BpuAI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AccII 82 CGCG CG^CG AcyI 29 GGCGTC G[GA]^CG[CT]C AflIII 130 ACGTGT A^C[GA][CT]GT AlwNI 1350 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 1444 GAGCAC G[AT]GC[AT]^C AvaII 228 GGACC G^G[AT]CC BalI 1491 TGGCCA TGG^CCA BbrPI 129 CACGTG CAC^GTG BcnI 404 CCCGG CC^[GC]GG BglI 971 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1234 AGATCT A^GATCT BlpI 529 GCTGAGC GC^T[ACGT]AGC BmyI 1444 GAGCAC G[AGT]GC[ACT]^C BpmI 107 CTGGAG CTGGAG(16/14) Bpu1102I 529 GCTGAGC GC^T[ACGT]AGC BsaAI 129 CACGTG [CT]AC^GT[GA] BsaHI 29 GGCGTC G[GA]^CG[CT]C BsaJI 845 CCTTGG C^C[ACGT][ACGT]GG BseRI 394 GAGGAG GAGGAG(10/8) BsiHKAI 1444 GAGCAC G[AT]GC[AT]^C BsmAI 550 GTCTC GTCTC(1/5) BsmI 1458 GAATGC GAATGC(1/-1) Bsp1286I 1444 GAGCAC G[AGT]GC[ACT]^C BspDI 1001 ATCGAT AT^CGAT BstUI 82 CGCG CG^CG BstXI 1200 CCAAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1234 AGATCT [GA]^GATC[CT] CelII 529 GCTGAGC GC^T[ACGT]AGC CfoI 83 GCGC GCG^C ClaI 1001 ATCGAT AT^CGAT DraIII 1447 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 583 GAAGAG CTCTTC(1/4) EcoNI 387 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 845 CCTTGG C^C[AT][AT]GG EcoT22I 700 ATGCAT ATGCA^T HapII 405 CCGG C^CGG HgaI 30 GCGTC GACGC(5/10) HhaI 83 GCGC GCG^C Hin1I 29 GGCGTC G[GA]^CG[CT]C HinP1I 83 GCGC G^CGC HincII 1361 GTCAAC GT[CT]^[GA]AC HindII 1361 GTCAAC GT[CT]^[GA]AC HpaII 405 CCGG C^CGG Ksp632I 583 GAAGAG CTCTTC(1/4) MaeIII 515 GTGAC ^GT[ACGT]AC MfeI 481 CAATTG C^AATTG MflI 1234 AGATCT [GA]^GATC[CT] MluNI 1491 TGGCCA TGG^CCA MscI 1491 TGGCCA TGG^CCA MslI 1183 CACAATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 104 CAGCTG C[AC]G^C[GT]G MspI 405 CCGG C^CGG MunI 481 CAATTG C^AATTG MvnI 82 CGCG CG^CG NciI 404 CCCGG CC^[GC]GG NsiI 700 ATGCAT ATGCA^T PmaCI 129 CACGTG CAC^GTG PmlI 129 CACGTG CAC^GTG PshAI 1354 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 101 CTGCAG CTGCA^G PvuII 104 CAGCTG CAG^CTG SphI 922 GCATGC GCATG^C StyI 845 CCTTGG C^C[AT][AT]GG Tsp45I 515 GTGAC ^GT[GC]AC XbaI 1126 TCTAGA T^CTAGA XhoII 1234 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 909 GGATC GGATC(4/5) 1024 GGATC BclI 207 TGATCA T^GATCA 867 TGATCA BsaBI 862 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1088 GATCAACATC BsiYI 100 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 387 CCTTCGTGAGG BslI 100 CCTGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 387 CCTTCGTGAGG BspHI 1440 TCATGA T^CATGA 1473 TCATGA DraII 978 AGGCCCC [GA]G^G[ACGT]CC[CT] 1390 AGGGCCT EaeI 802 TGGCCG [CT]^GGCC[GA] 1491 TGGCCA EcoO109I 978 AGGCCCC [GA]G^G[ACGT]CC[CT] 1390 AGGGCCT FbaI 207 TGATCA T^GATCA 867 TGATCA HphI 514 GGTGA GGTGA(8/7) 334 TCACC MaeII 130 ACGT A^CGT 1365 ACGT MamI 862 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1088 GATCAACATC NlaIV 979 GGCCCC GG[ACGT]^[ACGT]CC 1210 GGCTCC RcaI 1440 TCATGA T^CATGA 1473 TCATGA SfcI 101 CTGCAG C^T[GA][CT]AG 732 CTATAG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI BbvI BfaI BsmFI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I