(Circular) (Six-base) MAPSORT of: pFN21AE2459.seq Check: 4913 from: 1 to: 6220 pFN21AE2459 6220 bp With 182 enzymes: SgfI * February 8, 2013 19:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2411 2607 3192 4283 5667 5935 1632 Size: 779 196 585 1091 1384 268 1917 Fragments arranged by size: 1917 1384 1091 779 585 268 196 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 2135 5119 6096 1203 Size: 932 2984 977 1327 Fragments arranged by size: 2984 1327 977 932 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AlwNI CAG_nnn'CTG Cuts at: 3360 3963 5535 3360 Size: 603 1572 4045 Fragments arranged by size: 4045 1572 603 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 3529 3755 3907 6016 1493 Size: 2036 226 152 2109 1697 Fragments arranged by size: 2109 2036 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 3545 1870 Size: 73 1602 4545 Fragments arranged by size: 4545 1602 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2151 2184 2466 2499 2151 Size: 33 282 33 5872 Fragments arranged by size: 5872 282 33 33 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3541 4996 618 Size: 325 217 627 1754 1455 1842 Fragments arranged by size: 1842 1754 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3539 1391 Size: 2148 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4411 4445 1903 Size: 34 50 34 2390 34 3678 Fragments arranged by size: 3678 2390 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 2212 2872 1548 Size: 664 660 4896 Fragments arranged by size: 4896 664 660 BfrBI ATG'CAT Cuts at: 2707 2707 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2982 4831 136 Size: 107 122 71 631 605 1310 1849 1525 Fragments arranged by size: 1849 1525 1310 631 605 122 107 71 BglII A'GATC_T Cuts at: 3239 6215 3239 Size: 2976 3244 BlpI GC'TnA_GC Cuts at: 2535 3637 2535 Size: 1102 5118 Bme1580I G_kGCm'C Cuts at: 1790 4281 5437 1790 Size: 2491 1156 2573 Fragments arranged by size: 2573 2491 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3951 4909 442 Size: 416 53 83 399 24 2534 958 1753 Fragments arranged by size: 2534 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3261 3261 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2133 4880 1239 Size: 894 2747 2579 Fragments arranged by size: 2747 2579 894 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4345 5210 5508 4345 Size: 865 298 5057 Fragments arranged by size: 5057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2136 4011 493 Size: 711 932 1875 2702 Fragments arranged by size: 2702 1875 932 711 BsaBI GATnn'nnATC Cuts at: 2871 3097 6214 2871 Size: 226 3117 2877 Fragments arranged by size: 3117 2877 226 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2035 4027 4409 5044 Size: 53 83 186 936 502 1992 382 635 Cuts at: 5044 275 Size: 1451 Fragments arranged by size: 1992 1451 936 635 502 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2754 2784 1178 Size: 30 165 30 1351 30 4614 Fragments arranged by size: 4614 1351 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 1186 Size: 1228 4992 BseYI C'CCAG_C Cuts at: 3116 5423 5919 3116 Size: 2307 496 3417 Fragments arranged by size: 3417 2307 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5647 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3453 4281 4366 5437 3453 Size: 828 85 1071 4236 Fragments arranged by size: 4236 1071 828 85 BsmI GAATG_Cn' Cuts at: 3469 3731 3824 3469 Size: 262 93 5865 Fragments arranged by size: 5865 262 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3453 3539 4281 4366 5437 1391 Size: 399 1663 86 742 85 1071 2174 Fragments arranged by size: 2174 1663 1071 742 399 86 85 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 3445 3478 4109 5839 3445 Size: 33 631 1730 3826 Fragments arranged by size: 3826 1730 631 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3575 844 Size: 2731 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4864 5051 1700 Size: 110 3054 187 2869 Fragments arranged by size: 3054 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6220 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstXI CCAn_nnnn'nTGG Cuts at: 3212 3212 Size: 6220 BstYI r'GATC_y Cuts at: 1972 3239 3550 3917 4302 4319 5771 5857 5869 Size: 1267 311 367 385 17 1452 86 12 Cuts at: 5869 6215 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1267 385 367 346 311 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5668 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2487 3736 4530 4550 2487 Size: 1249 794 20 4157 Fragments arranged by size: 4157 1249 794 20 ClaI AT'CG_AT Cuts at: 1554 3007 1554 Size: 1453 4767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DraIII CAC_nnn'GTG Cuts at: 3457 3457 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2807 3496 3667 Size: 54 1425 50 275 18 977 689 171 Cuts at: 3667 4556 6185 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 977 889 689 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2583 4150 1353 Size: 1230 1567 3423 Fragments arranged by size: 3423 1567 1230 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2963 4807 5181 5327 387 Size: 1064 138 1374 1844 374 146 1280 Fragments arranged by size: 1844 1374 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2133 2411 2607 3192 4283 4880 5667 Size: 393 501 278 196 585 1091 597 787 Cuts at: 5667 5935 1239 Size: 268 1524 Fragments arranged by size: 1524 1091 787 597 585 501 393 278 268 196 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2396 Size: 6220 EcoO109I rG'GnC_Cy Cuts at: 2984 3396 2984 Size: 412 5808 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2531 2531 Size: 6220 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 5367 1678 Size: 3689 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2973 2989 3005 Size: 32 135 32 536 32 833 16 16 Cuts at: 3005 3021 3283 3315 4861 4893 4935 4967 1373 Size: 16 262 32 1546 32 42 32 2626 Fragments arranged by size: 2626 1546 833 536 262 135 42 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 3368 3564 3810 669 Size: 988 281 1430 196 246 3079 Fragments arranged by size: 3079 1430 988 281 246 196 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 2486 3819 2486 Size: 1333 4887 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2277 5334 5518 1305 Size: 972 3057 184 2007 Fragments arranged by size: 3057 2007 972 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3498 10 Size: 54 1425 343 1666 2732 Fragments arranged by size: 2732 1666 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3192 4178 4537 4696 518 Size: 648 2026 986 359 159 2042 Fragments arranged by size: 2042 2026 986 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2111 3636 4313 5461 5706 1606 Size: 505 1525 677 1148 245 2120 Fragments arranged by size: 2120 1525 1148 677 505 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5668 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NsiI A_TGCA'T Cuts at: 2709 2709 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2057 2711 2931 3159 3578 5123 1132 Size: 97 828 654 220 228 419 1545 2229 Fragments arranged by size: 2229 1545 828 654 419 228 220 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 5119 5119 Size: 6220 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6220 PfoI T'CCnGG_A Cuts at: 3313 3313 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PmlI CAC'GTG Cuts at: 2136 2136 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3363 1613 Size: 1750 4470 PsiI TTA'TAA Cuts at: 2499 3790 2499 Size: 1291 4929 PstI C_TGCA'G Cuts at: 830 2110 3572 830 Size: 1280 1462 3478 Fragments arranged by size: 3478 1462 1280 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2111 2111 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SfcI C'TryA_G Cuts at: 826 1045 2106 2737 3568 4703 5384 5575 826 Size: 219 1061 631 831 1135 681 191 1471 Fragments arranged by size: 1471 1135 1061 831 681 631 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4324 5225 5487 820 Size: 197 926 2381 901 262 1553 Fragments arranged by size: 2381 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 2931 3578 1132 Size: 1799 647 3774 Fragments arranged by size: 3774 1799 647 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2850 3659 513 Size: 552 1785 809 3074 Fragments arranged by size: 3074 1785 809 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2028 4247 4264 4417 4602 5047 325 Size: 1255 448 2219 17 153 185 445 1498 Fragments arranged by size: 2219 1498 1255 448 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2086 2118 6180 6212 2086 Size: 32 4062 32 2094 Fragments arranged by size: 4062 2094 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3131 3556 3131 Size: 425 5795 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 4349 4349 Size: 6220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI