################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeIII MamI MfeI MluI MroI MspA1I MunI MvaI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AclI 536 AACGTT AA^CGTT AcsI 525 AAATTC [GA]^AATT[CT] ApoI 525 AAATTC [GA]^AATT[CT] AspHI 665 GTGCTC G[AT]GC[AT]^C AvaII 655 GGTCC G^G[AT]CC BalI 2 TGGCCA TGG^CCA BanI 32 GGTGCC G^G[CT][GA]CC BcnI 67 CCCGG CC^[GC]GG BglII 647 AGATCT A^GATCT BmyI 665 GTGCTC G[AGT]GC[ACT]^C BpmI 254 CTGGAG CTGGAG(16/14) BsaJI 202 CCATGG C^C[ACGT][ACGT]GG BseRI 86 GAGGAG GAGGAG(10/8) BsgI 80 GTGCAG GTGCAG(16/14) BsiHKAI 665 GTGCTC G[AT]GC[AT]^C BsmAI 639 GTCTC GTCTC(1/5) BsmFI 656 GTCCC GGGAC(10/14) Bsp1286I 665 GTGCTC G[AGT]GC[ACT]^C BsrFI 29 GCCGGT [GA]^CCGG[CT] BssKI 67 CCCGG ^CC[ACGT]GG BstYI 647 AGATCT [GA]^GATC[CT] Cfr10I 29 GCCGGT [GA]^CCGG[CT] Cfr13I 655 GGTCC G^G[ACGT]CC DsaI 202 CCATGG C^C[GA][CT]GG EaeI 2 TGGCCA [CT]^GGCC[GA] EcoT14I 202 CCATGG C^C[AT][AT]GG MflI 647 AGATCT [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MslI 64 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 67 CCCGG CC^[GC]GG NcoI 202 CCATGG C^CATGG NlaIII 203 CATG CATG^ PflMI 197 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 536 AACGTT AA^CGTT Sau96I 655 GGTCC G^G[ACGT]CC ScrFI 67 CCCGG CC^[ACGT]GG SspI 436 AATATT AAT^ATT StyI 202 CCATGG C^C[AT][AT]GG Van91I 197 CCACACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 647 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 36 CCGC CCGC(-3/-1) 103 CCGC AluI 258 AGCT AG^CT 603 AGCT BfaI 74 CTAG C^TAG 149 CTAG BsrI 182 CCAGT ACTGG(1/-1) 418 CCAGT DdeI 293 CTGAG C^T[ACGT]AG 313 CTTAG HaeIII 3 GGCC GG^CC 28 GGCC HapII 30 CCGG C^CGG 68 CCGG HinfI 250 GATTC G^A[ACGT]TC 382 GAATC HpaII 30 CCGG C^CGG 68 CCGG HphI 415 TCACC GGTGA(8/7) 460 TCACC MaeI 74 CTAG C^TAG 149 CTAG MaeII 132 ACGT A^CGT 537 ACGT MboII 621 GAAGA GAAGA(8/7) 17 TCTTC MseI 496 TTAA T^TAA 541 TTAA MspI 30 CCGG C^CGG 68 CCGG NlaIV 32 GGTGCC GG[ACGT]^[ACGT]CC 655 GGTCCC TfiI 250 GATTC G^A[AT]TC 382 GAATC Tru9I 496 TTAA T^TAA 541 TTAA TspRI 394 CAGTG CA[GC]TG[ACGT][ACGT]^ 419 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### BbvI BsiYI BslI BstF5I DpnII Fnu4HI FokI ItaI MboI MnlI MwoI NdeII Sau3AI TseI Tsp509I