################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccIII AclI AfaI AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI BstYI Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI HphI KasI KpnI KspI MamI MboI MfeI MflI MluNI MroI MscI MunI NarI NcoI NdeI NdeII NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 336 GTATAC GT^[AC][GT]AC AcsI 381 GAATTC [GA]^AATT[CT] AcyI 59 GACGCC G[GA]^CG[CT]C AflII 466 CTTAAG C^TTAAG AflIII 213 ACGCGT A^C[GA][CT]GT ApoI 381 GAATTC [GA]^AATT[CT] AvaI 250 CTCGAG C^[CT]CG[GA]G AvaII 294 GGACC G^G[AT]CC BcgI 147 CGAAGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 73 CCCGG CC^[GC]GG BfaI 106 CTAG C^TAG BfrI 466 CTTAAG C^TTAAG BmyI 328 GTGCCC G[AGT]GC[ACT]^C BsaHI 59 GACGCC G[GA]^CG[CT]C BsiEI 9 CGGCCG CG[GA][CT]^CG BsmFI 435 GGGAC GGGAC(10/14) BsoBI 250 CTCGAG C^[CT]CG[GA]G Bsp1286I 328 GTGCCC G[AGT]GC[ACT]^C BsrFI 274 GCCGGC [GA]^CCGG[CT] Bst1107I 336 GTATAC GTA^TAC BstZ17I 336 GTATAC GTA^TAC Cfr10I 274 GCCGGC [GA]^CCGG[CT] DsaI 297 CCACGG C^C[GA][CT]GG EaeI 9 CGGCCG [CT]^GGCC[GA] EagI 9 CGGCCG C^GGCCG EarI 4 CTCTTC CTCTTC(1/4) EclXI 9 CGGCCG C^GGCCG Eco52I 9 CGGCCG C^GGCCG EcoRI 381 GAATTC G^AATTC EcoT14I 290 CCAAGG C^C[AT][AT]GG Hin1I 59 GACGCC G[GA]^CG[CT]C Ksp632I 4 CTCTTC CTCTTC(1/4) MaeI 106 CTAG C^TAG MaeII 197 ACGT A^CGT MaeIII 411 GTTAC ^GT[ACGT]AC MboII 5 TCTTC GAAGA(8/7) MluI 213 ACGCGT A^CGCGT MseI 467 TTAA T^TAA MspA1I 19 CCGCTG C[AC]G^C[GT]G NaeI 274 GCCGGC GCC^GGC NciI 73 CCCGG CC^[GC]GG NgoMIV 274 GCCGGC G^CCGGC PaeR7I 250 CTCGAG C^TCGAG PstI 442 CTGCAG CTGCA^G SapI 3 GCTCTTC GCTCTTC(1/4) SfcI 442 CTGCAG C^T[GA][CT]AG StyI 290 CCAAGG C^C[AT][AT]GG Tru9I 467 TTAA T^TAA Tsp509I 382 AATT ^AATT TspRI 177 CACTG CA[GC]TG[ACGT][ACGT]^ XhoI 250 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BseRI 136 GAGGAG GAGGAG(10/8) 253 GAGGAG BstF5I 15 CATCC GGATG(2/0) 348 GGATG FokI 15 CATCC GGATG(9/13) 348 GGATG HapII 74 CCGG C^CGG 275 CCGG HinfI 80 GATTC G^A[ACGT]TC 471 GATTC HpaII 74 CCGG C^CGG 275 CCGG MslI 33 CATACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 226 CACTTCTATG MspI 74 CCGG C^CGG 275 CCGG PflMI 297 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 426 CCAGGACTTGG SmlI 250 CTCGAG C^T[CT][GA]AG 466 CTTAAG StuI 360 AGGCCT AGG^CCT 462 AGGCCT TaqI 53 TCGA T^CGA 251 TCGA TfiI 80 GATTC G^A[AT]TC 471 GATTC TthHB8I 53 TCGA T^CGA 251 TCGA Van91I 297 CCACGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 426 CCAGGACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AccII AciI AluI BbvI BpmI BsaJI BsiYI BslI BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeIII HgaI HhaI HinP1I ItaI MnlI MvaI MvnI MwoI Sau96I ScrFI SfaNI TseI