(Linear) (Six-base) MAPSORT of: KIEE5170.seq Check: 190 from: 1 to: 486 KIEE5170.orf With 182 enzymes: SgfI * January 29, 2014 11:49 .. AccI GT'mk_AC Cuts at: 0 337 486 Size: 337 149 AflII C'TTAA_G Cuts at: 0 466 486 Size: 466 20 AflIII A'CryG_T Cuts at: 0 213 486 Size: 213 273 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 37 486 Size: 37 449 ApoI r'AATT_y Cuts at: 0 381 486 Size: 381 105 AvaI C'yCGr_G Cuts at: 0 250 486 Size: 250 236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 136 170 486 Size: 136 34 316 Fragments arranged by size: 316 136 34 Bme1580I G_kGCm'C Cuts at: 0 332 486 Size: 332 154 BmgBI CAC'GTC Cuts at: 0 198 486 Size: 198 288 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 397 409 486 Size: 325 72 12 77 Fragments arranged by size: 325 77 72 12 BsaHI Gr'CG_yC Cuts at: 0 60 486 Size: 60 426 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 175 205 486 Size: 175 30 281 Fragments arranged by size: 281 175 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 151 268 486 Size: 151 117 218 Fragments arranged by size: 218 151 117 BseYI C'CCAG_C Cuts at: 0 141 312 486 Size: 141 171 174 Fragments arranged by size: 174 171 141 BsiEI CG_ry'CG Cuts at: 0 12 486 Size: 12 474 Bsp1286I G_dGCh'C Cuts at: 0 332 486 Size: 332 154 BsrFI r'CCGG_y Cuts at: 0 274 486 Size: 274 212 BstZ17I GTA'TAC Cuts at: 0 338 486 Size: 338 148 BtgI C'CryG_G Cuts at: 0 297 486 Size: 297 189 EaeI y'GGCC_r Cuts at: 0 9 486 Size: 9 477 EagI C'GGCC_G Cuts at: 0 9 486 Size: 9 477 EarI CTCTTCn'nnn_ Cuts at: 0 10 486 Size: 10 476 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 92 486 Size: 92 394 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 397 409 486 Size: 325 72 12 77 Fragments arranged by size: 325 77 72 12 EcoRI G'AATT_C Cuts at: 0 381 486 Size: 381 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 462 486 Size: 462 24 MluI A'CGCG_T Cuts at: 0 213 486 Size: 213 273 MslI CAynn'nnrTG Cuts at: 0 37 230 486 Size: 37 193 256 Fragments arranged by size: 256 193 37 MspA1I CmG'CkG Cuts at: 0 21 486 Size: 21 465 NaeI GCC'GGC Cuts at: 0 276 486 Size: 276 210 NgoMIV G'CCGG_C Cuts at: 0 274 486 Size: 274 212 PflMI CCAn_nnn'nTGG Cuts at: 0 303 432 486 Size: 303 129 54 Fragments arranged by size: 303 129 54 PfoI T'CCnGG_A Cuts at: 0 374 425 486 Size: 374 51 61 Fragments arranged by size: 374 61 51 PspXI vC'TCGA_Gb Cuts at: 0 250 486 Size: 250 236 PstI C_TGCA'G Cuts at: 0 446 486 Size: 446 40 SapI GCTCTTCn'nnn_ Cuts at: 0 10 486 Size: 10 476 SfcI C'TryA_G Cuts at: 0 442 486 Size: 442 44 SmlI C'TyrA_G Cuts at: 0 250 466 486 Size: 250 216 20 Fragments arranged by size: 250 216 20 StuI AGG'CCT Cuts at: 0 362 464 486 Size: 362 102 22 Fragments arranged by size: 362 102 22 StyI C'CwwG_G Cuts at: 0 290 486 Size: 290 196 XhoI C'TCGA_G Cuts at: 0 250 486 Size: 250 236 Enzymes that do cut: AccI AflII AflIII AlfI ApoI AvaI BcgI Bme1580I BmgBI BpmI BsaHI BsaXI BseRI BseYI BsiEI Bsp1286I BsrFI BstZ17I BtgI EaeI EagI EarI EciI Eco57MI EcoRI Hin4I MluI MslI MspA1I NaeI NgoMIV PflMI PfoI PspXI PstI SapI SfcI SmlI StuI StyI XhoI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI