################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbvCI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI Hin1I HincII HindII HpaI KasI KpnI MaeI MamI MflI MluI MluNI MroI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 340 GTCTAC GT^[AC][GT]AC AccII 109 CGCG CG^CG Alw44I 277 GTGCAC G^TGCAC AlwNI 409 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 277 GTGCAC G^TGCAC AvaI 1208 CTCGGG C^[CT]CG[GA]G BanII 356 GAGCCC G[GA]GC[CT]^C BbsI 1141 GTCTTC GAAGAC(2/6) BcgI 1172 GCACACCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 95 TGATCA T^GATCA BcnI 150 CCGGG CC^[GC]GG BpuAI 1141 GTCTTC GAAGAC(2/6) BseRI 76 GAGGAG GAGGAG(10/8) BsmAI 961 GAGAC GTCTC(1/5) BsoBI 1208 CTCGGG C^[CT]CG[GA]G Bsp1407I 862 TGTACA T^GTACA BsrGI 862 TGTACA T^GTACA BsrI 753 CCAGT ACTGG(1/-1) BstAPI 1110 GCATCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 109 CGCG CG^CG DsaI 108 CCGCGG C^C[GA][CT]GG EcoNI 423 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 110 GCGGG CCCGC(4/6) FbaI 95 TGATCA T^GATCA HaeII 410 AGCGCC [GA]GCGC^[CT] HgaI 1139 GCGTC GACGC(5/10) HindIII 18 AAGCTT A^AGCTT KspI 108 CCGCGG CCGC^GG MfeI 221 CAATTG C^AATTG MslI 828 CATAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 221 CAATTG C^AATTG MvnI 109 CGCG CG^CG NciI 150 CCGGG CC^[GC]GG PleI 739 GACTC GAGTC(4/5) PpuMI 918 GGGACCT [GA]G^G[AT]CC[CT] PshAI 715 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 156 CAGCTG CAG^CTG SacII 108 CCGCGG CCGC^GG SbfI 360 CCTGCAGG CCTGCA^GG SfaNI 1110 GCATC GCATC(5/9) SphI 728 GCATGC GCATG^C Sse8387I 360 CCTGCAGG CCTGCA^GG SspBI 862 TGTACA T^GTACA Tsp45I 1214 GTGAC ^GT[GC]AC XcmI 1187 CCAGGTTCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 863 GTAC GT^AC 910 GTAC AflIII 281 ACACGT A^C[GA][CT]GT 1153 ACGTGT AlwI 573 GGATC GGATC(4/5) 1121 GGATC AvaII 147 GGTCC G^G[AT]CC 919 GGACC BbrPI 791 CACGTG CAC^GTG 1152 CACGTG BsaAI 791 CACGTG [CT]AC^GT[GA] 1152 CACGTG BsmFI 152 GGGAC GGGAC(10/14) 918 GGGAC BsmI 192 GAATGC GAATGC(1/-1) 833 GAATGC BsrFI 137 GCCGGC [GA]^CCGG[CT] 404 GCCGGC BssSI 73 CACGAG CACGAG(-5/-1) 1180 CTCGTG CfoI 85 GCGC GCG^C 411 GCGC Cfr10I 137 GCCGGC [GA]^CCGG[CT] 404 GCCGGC DraII 419 AGGCCCT [GA]G^G[ACGT]CC[CT] 918 GGGACCT EcoO109I 419 AGGCCCT [GA]G^G[ACGT]CC[CT] 918 GGGACCT HhaI 85 GCGC GCG^C 411 GCGC HinP1I 85 GCGC G^CGC 411 GCGC HphI 1213 GGTGA GGTGA(8/7) 801 TCACC NaeI 137 GCCGGC GCC^GGC 404 GCCGGC NgoMIV 137 GCCGGC G^CCGGC 404 GCCGGC NspI 600 ACATGC [GA]CATG^[CT] 728 GCATGC PmaCI 791 CACGTG CAC^GTG 1152 CACGTG PmlI 791 CACGTG CAC^GTG 1152 CACGTG RsaI 863 GTAC GT^AC 910 GTAC TfiI 546 GATTC G^A[AT]TC 778 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcsI AluI ApoI AspHI BbvI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfcI Tru9I TseI Tsp509I TspRI