Restriction map for
pFN21AE6391
(Circular) (Six-base) MAPSORT of: pFN21AE6391.seq Check: 9505 from: 1 to: 5947
pFN21AE6391 5947 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2346 3290 1937
Size: 409 944 4594
Fragments arranged by size:
4594 944 409
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2913 4010 5394 5662 1632
Size: 1281 1097 1384 268 1917
Fragments arranged by size:
1917 1384 1281 1097 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 2286 3158 4846 5823 1203
Size: 1083 872 1688 977 1327
Fragments arranged by size:
1688 1327 1083 977 872
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5947
AlwNI CAG_nnn'CTG
Cuts at: 2419 3690 5262 2419
Size: 1271 1572 3104
Fragments arranged by size:
3104 1572 1271
ApaLI G'TGCA_C
Cuts at: 2282 4004 5160 2282
Size: 1722 1156 3069
Fragments arranged by size:
3069 1722 1156
ApoI r'AATT_y
Cuts at: 1493 2295 2326 2978 3017 3256 3482 3634 5743
Size: 802 31 652 39 239 226 152 2109
Cuts at: 5743 1493
Size: 1697
Fragments arranged by size:
2109 1697 802 652 239 226 152 39
31
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3213 3272 1870
Size: 73 1270 59 4545
Fragments arranged by size:
4545 1270 73 59
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3268 4723 618
Size: 325 217 627 1481 1455 1842
Fragments arranged by size:
1842 1481 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2365 3266 1391
Size: 974 901 4072
Fragments arranged by size:
4072 974 901
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3139 928
Size: 971 1240 3736
Fragments arranged by size:
3736 1240 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3166 3200 4138 4172 1903
Size: 34 1229 34 938 34 3678
Fragments arranged by size:
3678 1229 938 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 2100 1548
Size: 552 5395
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4558 136
Size: 107 122 71 631 605 2886 1525
Fragments arranged by size:
2886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 2286 4008 5164 1790
Size: 496 1722 1156 2573
Fragments arranged by size:
2573 1722 1156 496
BmgBI CAC'GTC
Cuts at: 1318 2248 1318
Size: 930 5017
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3678 4636 442
Size: 416 53 83 399 24 2261 958 1753
Fragments arranged by size:
2261 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 2606 3009 4607 1239
Size: 1202 165 403 1598 2579
Fragments arranged by size:
2579 1598 1202 403 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4072 4937 5235 4072
Size: 865 298 4784
Fragments arranged by size:
4784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4610 882
Size: 617 3111 2219
Fragments arranged by size:
3111 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2798 3159 3738 493
Size: 711 1594 361 579 2702
Fragments arranged by size:
2702 1594 711 579 361
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 1186
Size: 910 5037
BseYI C'CCAG_C
Cuts at: 5150 5646 5150
Size: 496 5451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2084 2093 2820 1163
Size: 573 348 9 727 4290
Fragments arranged by size:
4290 727 573 348 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2286 3214 4008 4093 5164 2079
Size: 207 928 794 85 1071 2862
Fragments arranged by size:
2862 1071 928 794 207 85
BsmI GAATG_Cn'
Cuts at: 2203 2844 3458 3551 2203
Size: 641 614 93 4599
Fragments arranged by size:
4599 641 614 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2286 2365 3214 3266 4008 4093
Size: 399 289 207 79 849 52 742 85
Cuts at: 4093 5164 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 849 742 399 289 207 85
79 52
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2142 2409 4591 4778 1700
Size: 110 332 267 2182 187 2869
Fragments arranged by size:
2869 2182 332 267 187 110
BsrGI T'GTAC_A
Cuts at: 96 2867 96
Size: 2771 3176
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 2078 3185 4007 5019 2078
Size: 1107 822 1012 3006
Fragments arranged by size:
3006 1107 1012 822
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3121 794
Size: 2327 3620
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942
Size: 1305 367 385 17 1452 86 12 346
Cuts at: 5942 1972
Size: 1977
Fragments arranged by size:
1977 1452 1305 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2113 513
Size: 552 1048 4347
Fragments arranged by size:
4347 1048 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 2153 2241 3214 3463 4257 4277 2153
Size: 88 973 249 794 20 3823
Fragments arranged by size:
3823 973 794 249 88 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912
Size: 54 1425 50 275 18 1564 889 1629
Cuts at: 5912 8
Size: 43
Fragments arranged by size:
1629 1564 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2259 2586 2667 3877 1353
Size: 906 327 81 1210 3423
Fragments arranged by size:
3423 1210 906 327 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2177 4534 4908 5054 387
Size: 1064 138 588 2357 374 146 1280
Fragments arranged by size:
2357 1280 1064 588 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2441 2606 2913 3009 4010 4607 5394
Size: 393 809 165 307 96 1001 597 787
Cuts at: 5394 5662 1239
Size: 268 1524
Fragments arranged by size:
1524 1001 809 787 597 393 307 268
165 96
EcoNI CCTnn'n_nnAGG
Cuts at: 2432 2432
Size: 5947
EcoO109I rG'GnC_Cy
Cuts at: 2425 2924 2425
Size: 499 5448
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3073 3073
Size: 5947
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 2419 5094 1678
Size: 741 2675 2531
Fragments arranged by size:
2675 2531 741
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 4588 4620 4662
Size: 32 135 32 1037 32 1947 32 42
Cuts at: 4662 4694 1373
Size: 32 2626
Fragments arranged by size:
2626 1947 1037 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3291 3537 669
Size: 988 281 1353 246 3079
Fragments arranged by size:
3079 1353 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2023 748
Size: 1275 4672
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 2226 3546 2226
Size: 1320 4627
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2238 5061 5245 1305
Size: 933 2823 184 2007
Fragments arranged by size:
2823 2007 933 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2837 3905 4264 4423 518
Size: 648 843 828 1068 359 159 2042
Fragments arranged by size:
2042 1068 843 828 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2115 2163 2576 3363 4040 5188 5433 1606
Size: 509 48 413 787 677 1148 245 2120
Fragments arranged by size:
2120 1148 787 677 509 413 245 48
NaeI GCC'GGC
Cuts at: 2144 2411 2144
Size: 267 5680
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5395
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NgoMIV G'CCGG_C
Cuts at: 2142 2409 2142
Size: 267 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2609 2737 3305 4850 1132
Size: 97 1380 128 568 1545 2229
Fragments arranged by size:
2229 1545 1380 568 128 97
PasI CC'CwG_GG
Cuts at: 1348 2055 1348
Size: 707 5240
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PfoI T'CCnGG_A
Cuts at: 2154 2154
Size: 5947
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PmlI CAC'GTG
Cuts at: 2798 3159 2798
Size: 361 5586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2924 2924
Size: 5947
PshAI GACnn'nnGTC
Cuts at: 1613 2724 1613
Size: 1111 4836
PsiI TTA'TAA
Cuts at: 2816 3517 2816
Size: 701 5246
PstI C_TGCA'G
Cuts at: 830 2168 2358 2370 3299 830
Size: 1338 190 12 929 3478
Fragments arranged by size:
3478 1338 929 190 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
PvuII CAG'CTG
Cuts at: 2163 2163
Size: 5947
SacII CC_GC'GG
Cuts at: 2116 2116
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SbfI CC_TGCA'GG
Cuts at: 2370 3299 2370
Size: 929 5018
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2164 2354 2366 3295 4430 5111 5302
Size: 219 1119 190 12 929 1135 681 191
Cuts at: 5302 826
Size: 1471
Fragments arranged by size:
1471 1135 1119 929 681 219 191 190
12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4051 4952 5214 820
Size: 197 926 2108 901 262 1553
Fragments arranged by size:
2108 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 2737 3305 1132
Size: 1605 568 3774
Fragments arranged by size:
3774 1605 568
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 3386 513
Size: 552 2321 3074
Fragments arranged by size:
3074 2321 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2023 3974 3991 4144 4329 4774 325
Size: 1255 443 1951 17 153 185 445 1498
Fragments arranged by size:
1951 1498 1255 445 443 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2867 2914 4193
Size: 274 80 33 51 1443 890 47 1279
Cuts at: 4193 5905 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1279 890 274 138 80 51
47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3199 2011
Size: 1188 4759
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 4076 4076
Size: 5947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI