################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 190 AAATTC [GA]^AATT[CT] AfaI 135 GTAC GT^AC ApoI 190 AAATTC [GA]^AATT[CT] Asp700I 483 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 866 GGCACC G^G[CT][GA]CC BbsI 424 GAAGAC GAAGAC(2/6) BcgI 791 CGAAAATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 642 GTATCC GTATCC(6/5) BclI 326 TGATCA T^GATCA BcnI 825 CCGGG CC^[GC]GG BpuAI 424 GAAGAC GAAGAC(2/6) BseRI 418 GAGGAG GAGGAG(10/8) BsgI 161 CTGCAC GTGCAG(16/14) BstF5I 852 CATCC GGATG(2/0) CfoI 392 GCGC GCG^C DpnII 327 GATC ^GATC DraII 248 AGGACCT [GA]G^G[ACGT]CC[CT] EcoNI 401 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 248 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 397 CCCGC CCCGC(4/6) FbaI 326 TGATCA T^GATCA FokI 852 CATCC GGATG(9/13) HaeII 391 GGCGCT [GA]GCGC^[CT] HapII 825 CCGG C^CGG HgaI 582 GACGC GACGC(5/10) HhaI 392 GCGC GCG^C HinP1I 392 GCGC G^CGC HpaII 825 CCGG C^CGG MaeII 620 ACGT A^CGT MboI 327 GATC ^GATC MspI 825 CCGG C^CGG NciI 825 CCGGG CC^[GC]GG NdeII 327 GATC ^GATC NspI 467 ACATGC [GA]CATG^[CT] PleI 509 GAGTC GAGTC(4/5) PpuMI 248 AGGACCT [GA]G^G[AT]CC[CT] RsaI 135 GTAC GT^AC Sau3AI 327 GATC ^GATC SfaNI 798 GATGC GCATC(5/9) SmlI 878 CTCAAG C^T[CT][GA]AG StuI 819 AGGCCT AGG^CCT TfiI 205 GATTC G^A[AT]TC Tsp509I 191 AATT ^AATT XmnI 483 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaII 29 GGACC G^G[AT]CC 249 GGACC BanII 127 GAGCCC G[GA]GC[CT]^C 542 GAGCCC BfaI 38 CTAG C^TAG 506 CTAG BmyI 127 GAGCCC G[AGT]GC[ACT]^C 542 GAGCCC BpmI 515 CTGGAG CTGGAG(16/14) 539 CTGGAG BsmFI 464 GGGAC GGGAC(10/14) 580 GGGAC Bsp1286I 127 GAGCCC G[AGT]GC[ACT]^C 542 GAGCCC Cac8I 309 GCAGGC GC[ACGT]^[ACGT]GC 400 GCCTGC EarI 90 CTCTTC CTCTTC(1/4) 95 CTCTTC HinfI 205 GATTC G^A[ACGT]TC 509 GAGTC Ksp632I 90 CTCTTC CTCTTC(1/4) 95 CTCTTC MaeI 38 CTAG C^TAG 506 CTAG MseI 521 TTAA T^TAA 605 TTAA MslI 709 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 789 CACGAAAATG Tru9I 521 TTAA T^TAA 605 TTAA TspRI 273 CAGTG CA[GC]TG[ACGT][ACGT]^ 366 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI Cfr13I DdeI EcoRII Fnu4HI HaeIII HphI ItaI MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI