################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmFI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII Hin1I HindIII HpaI HphI KasI KpnI KspI MaeII MamI MfeI MluI MroI MseI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AflIII 596 ACATGT A^C[GA][CT]GT AluI 848 AGCT AG^CT AlwI 856 GATCC GGATC(4/5) AocI 135 CCTCAGG CC^T[ACGT]AGG ApaI 302 GGGCCC GGGCC^C Asp700I 246 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 52 GTGCTC G[AT]GC[AT]^C AvaI 68 CTCGGG C^[CT]CG[GA]G BalI 809 TGGCCA TGG^CCA BbsI 580 GTCTTC GAAGAC(2/6) BbvCI 639 CCTCAGC CCTCAGC(-5/-2) BcgI 98 CGACCGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 661 CTAG C^TAG BpuAI 580 GTCTTC GAAGAC(2/6) BseRI 267 CTCCTC GAGGAG(10/8) BsgI 818 GTGCAG GTGCAG(16/14) BsiEI 98 CGACCG CG[GA][CT]^CG BsiHKAI 52 GTGCTC G[AT]GC[AT]^C BsmBI 220 CGTCTC CGTCTC(1/5) BsmI 82 GAATGC GAATGC(1/-1) BsoBI 68 CTCGGG C^[CT]CG[GA]G BspLU11I 596 ACATGT A^CATGT BspMI 167 ACCTGC ACCTGC(4/8) BsrBI 65 CCGCTC CCGCTC(-3/-3) BsrDI 684 CATTGC GCAATG(2/0) BstAPI 699 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 855 AGATCC [GA]^GATC[CT] Bsu36I 135 CCTCAGG CC^T[ACGT]AGG CpoI 61 CGGACCG CG^G[AT]CCG EarI 278 CTCTTC CTCTTC(1/4) Eco81I 135 CCTCAGG CC^T[ACGT]AGG EcoT14I 738 CCTTGG C^C[AT][AT]GG EcoT22I 566 ATGCAT ATGCA^T FauI 27 GCGGG CCCGC(4/6) HgaI 702 GACGC GACGC(5/10) HincII 471 GTCAAC GT[CT]^[GA]AC HindII 471 GTCAAC GT[CT]^[GA]AC HinfI 587 GAATC G^A[ACGT]TC Ksp632I 278 CTCTTC CTCTTC(1/4) MaeI 661 CTAG C^TAG MflI 855 AGATCC [GA]^GATC[CT] MluNI 809 TGGCCA TGG^CCA MscI 809 TGGCCA TGG^CCA NruI 78 TCGCGA TCG^CGA NsiI 566 ATGCAT ATGCA^T NspI 596 ACATGT [GA]CATG^[CT] RsrII 61 CGGACCG CG^G[AT]CCG ScaI 237 AGTACT AGT^ACT SmlI 872 CTCAAG C^T[CT][GA]AG StyI 738 CCTTGG C^C[AT][AT]GG TfiI 587 GAATC G^A[AT]TC XhoII 855 AGATCC [GA]^GATC[CT] XmnI 246 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 79 CGCG CG^CG 102 CGCG AfaI 165 GTAC GT^AC 238 GTAC AlwNI 346 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 700 CAGACGCTG AvaII 62 GGACC G^G[AT]CC 72 GGTCC BanII 148 GAGCCC G[GA]GC[CT]^C 302 GGGCCC BcnI 10 CCCGG CC^[GC]GG 15 CCCGG BsaJI 689 CCCTGG C^C[ACGT][ACGT]GG 738 CCTTGG BsiYI 131 CCAGCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 639 CCTCAGCATGG BslI 131 CCAGCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 639 CCTCAGCATGG BsmAI 21 GTCTC GTCTC(1/5) 221 GTCTC BstF5I 569 CATCC GGATG(2/0) 3 GGATG BstUI 79 CGCG CG^CG 102 CGCG Cac8I 27 GCGGGC GC[ACGT]^[ACGT]GC 130 GCCAGC CfoI 103 GCGC GCG^C 113 GCGC DpnII 768 GATC ^GATC 856 GATC DraII 302 GGGCCCT [GA]G^G[ACGT]CC[CT] 347 AGGGCCT EaeI 44 CGGCCA [CT]^GGCC[GA] 809 TGGCCA EcoO109I 302 GGGCCCT [GA]G^G[ACGT]CC[CT] 347 AGGGCCT FokI 569 CATCC GGATG(9/13) 3 GGATG HhaI 103 GCGC GCG^C 113 GCGC HinP1I 103 GCGC G^CGC 113 GCGC MboI 768 GATC ^GATC 856 GATC MvnI 79 CGCG CG^CG 102 CGCG NciI 10 CCCGG CC^[GC]GG 15 CCCGG NdeII 768 GATC ^GATC 856 GATC PstI 521 CTGCAG CTGCA^G 697 CTGCAG RsaI 165 GTAC GT^AC 238 GTAC Sau3AI 768 GATC ^GATC 856 GATC SfcI 521 CTGCAG C^T[GA][CT]AG 697 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI BbvI BmyI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HpaII ItaI MaeIII MboII MnlI MslI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TseI Tsp509I TspRI