(Circular) (Six-base) MAPSORT of: pFN21AE6287.seq Check: 1202 from: 1 to: 5593 pFN21AE6287 5593 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3403 278 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2936 1937 Size: 999 4594 Acc65I G'GTAC_C Cuts at: 2914 2914 Size: 5593 AclI AA'CG_TT Cuts at: 1563 3720 4093 1563 Size: 2157 373 3063 Fragments arranged by size: 3063 2157 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2572 2874 3656 5040 5308 1632 Size: 940 302 782 1384 268 1917 Fragments arranged by size: 1917 1384 940 782 302 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5396 AflIII A'CryG_T Cuts at: 1203 2601 4492 5469 1203 Size: 1398 1891 977 1327 Fragments arranged by size: 1891 1398 1327 977 AgeI A'CCGG_T Cuts at: 4424 4424 Size: 5593 AhdI GACnn_n'nnGTC Cuts at: 4322 4322 Size: 5593 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5593 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5593 AlwNI CAG_nnn'CTG Cuts at: 2356 2710 3336 4908 2356 Size: 354 626 1572 3041 Fragments arranged by size: 3041 1572 626 354 ApaI G_GGCC'C Cuts at: 2311 2311 Size: 5593 ApaLI G'TGCA_C Cuts at: 3650 4806 3650 Size: 1156 4437 ApoI r'AATT_y Cuts at: 1493 2902 3128 3280 5389 1493 Size: 1409 226 152 2109 1697 Fragments arranged by size: 2109 1697 1409 226 152 AseI AT'TA_AT Cuts at: 160 1032 4147 160 Size: 872 3115 1606 Fragments arranged by size: 3115 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2918 1870 Size: 73 130 845 4545 Fragments arranged by size: 4545 845 130 73 BamHI G'GATC_C Cuts at: 2923 3290 2923 Size: 367 5226 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2914 4369 618 Size: 325 217 627 1127 1455 1842 Fragments arranged by size: 1842 1455 1127 627 325 217 BanII G_rGCy'C Cuts at: 1391 2157 2311 2912 1391 Size: 766 154 601 4072 Fragments arranged by size: 4072 766 601 154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2578 928 Size: 971 679 3943 Fragments arranged by size: 3943 971 679 BbvCI CC'TCA_GC Cuts at: 2645 2645 Size: 5593 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2092 2126 3784 3818 1903 Size: 34 155 34 1658 34 3678 Fragments arranged by size: 3678 1658 155 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3485 4695 3485 Size: 1210 4383 BclI T'GATC_A Cuts at: 1548 1548 Size: 5593 BfrBI ATG'CAT Cuts at: 2573 2573 Size: 5593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4204 136 Size: 107 122 71 631 605 2532 1525 Fragments arranged by size: 2532 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5588 5588 Size: 5593 BlpI GC'TnA_GC Cuts at: 3010 3010 Size: 5593 Bme1580I G_kGCm'C Cuts at: 1790 2016 2311 3654 4810 1790 Size: 226 295 1343 1156 2573 Fragments arranged by size: 2573 1343 1156 295 226 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5593 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2438 3324 4282 Size: 416 53 83 399 24 1021 886 958 Cuts at: 4282 442 Size: 1753 Fragments arranged by size: 1753 1021 958 886 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5593 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4253 1239 Size: 3014 2579 Bpu10I CC'TnA_GC Cuts at: 2645 2645 Size: 5593 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2862 3718 4583 4881 2862 Size: 856 865 298 3574 Fragments arranged by size: 3574 865 856 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4256 882 Size: 617 2757 2219 Fragments arranged by size: 2757 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3384 493 Size: 711 2180 2702 Fragments arranged by size: 2702 2180 711 BsaBI GATnn'nnATC Cuts at: 5587 5587 Size: 5593 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3400 3782 4417 275 Size: 53 83 186 936 1867 382 635 1451 Fragments arranged by size: 1867 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4026 4424 4698 4845 5256 4026 Size: 398 274 147 411 4363 Fragments arranged by size: 4363 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5368 Fragments arranged by size: 5368 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2263 1186 Size: 1077 4516 BseYI C'CCAG_C Cuts at: 2176 4796 5292 2176 Size: 2620 496 2477 Fragments arranged by size: 2620 2477 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2844 1163 Size: 573 1108 3912 Fragments arranged by size: 3912 1108 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2106 3043 3804 4832 1540 Size: 275 186 105 937 761 1028 2301 Fragments arranged by size: 2301 1028 937 761 275 186 105 BsiHKAI G_wGCw'C Cuts at: 2061 3654 3739 4810 2061 Size: 1593 85 1071 2844 Fragments arranged by size: 2844 1593 1071 85 BsmI GAATG_Cn' Cuts at: 2093 3104 3197 2093 Size: 1011 93 4489 Fragments arranged by size: 4489 1011 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2231 2231 Size: 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2061 2157 2311 2912 3654 3739 Size: 399 226 45 96 154 601 742 85 Cuts at: 3739 4810 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 601 399 226 154 96 85 45 BspEI T'CCGG_A Cuts at: 5256 5256 Size: 5593 BspHI T'CATG_A Cuts at: 3482 5212 3482 Size: 1730 3863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2181 2948 844 Size: 1337 767 3489 Fragments arranged by size: 3489 1337 767 BsrBI CCG'CTC Cuts at: 1394 1823 2072 3480 1394 Size: 429 249 1408 3507 Fragments arranged by size: 3507 1408 429 249 BsrDI GCAATG_nn' Cuts at: 65 1248 2688 4088 4262 65 Size: 1183 1440 1400 174 1396 Fragments arranged by size: 1440 1400 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4237 4424 1700 Size: 110 2427 187 2869 Fragments arranged by size: 2869 2427 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5593 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5593 BssSI C'ACGA_G Cuts at: 3653 4665 3653 Size: 1012 4581 BstAPI GCAn_nnn'nTGC Cuts at: 794 2710 794 Size: 1916 3677 BstBI TT'CG_AA Cuts at: 4397 4397 Size: 5593 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5593 BstYI r'GATC_y Cuts at: 1972 2860 2923 3290 3675 3692 5144 5230 5242 Size: 888 63 367 385 17 1452 86 12 Cuts at: 5242 5588 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 888 385 367 346 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2141 2141 Size: 5593 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4102 Fragments arranged by size: 4102 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2782 487 Size: 683 56 1556 3298 Fragments arranged by size: 3298 1556 683 56 BtsI GCAGTG_nn' Cuts at: 2535 2699 3109 3903 3923 2535 Size: 164 410 794 20 4205 Fragments arranged by size: 4205 794 410 164 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5593 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5558 DraI TTT'AAA Cuts at: 2897 2897 Size: 5593 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4600 809 Size: 507 3284 1802 Fragments arranged by size: 3284 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2814 3040 Size: 54 1425 50 275 18 219 765 226 Cuts at: 3040 3929 5558 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 765 275 226 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3040 1537 Size: 275 1228 4090 Fragments arranged by size: 4090 1228 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 3523 1353 Size: 936 1234 3423 Fragments arranged by size: 3423 1234 936 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4180 4554 4700 387 Size: 1064 138 2591 374 146 1280 Fragments arranged by size: 2591 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2572 2874 3656 4253 5040 5308 1239 Size: 393 940 302 782 597 787 268 1524 Fragments arranged by size: 1524 940 787 782 597 393 302 268 EcoO109I rG'GnC_Cy Cuts at: 2308 2353 2308 Size: 45 5548 EcoRI G'AATT_C Cuts at: 2902 3280 2902 Size: 378 5215 FspI TGC'GCA Cuts at: 4099 4099 Size: 5593 HaeII r_GCGC'y Cuts at: 1678 4740 1678 Size: 3062 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2362 2394 2485 2517 2852 Size: 32 135 32 790 32 91 32 335 Cuts at: 2852 2884 4234 4266 4308 4340 1373 Size: 32 1350 32 42 32 2626 Fragments arranged by size: 2626 1350 790 335 135 91 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2478 2937 3183 669 Size: 988 281 540 459 246 3079 Fragments arranged by size: 3079 988 540 459 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5593 HpaI GTT'AAC Cuts at: 3183 3183 Size: 5593 KpnI G_GTAC'C Cuts at: 2918 2918 Size: 5593 MfeI C'AATT_G Cuts at: 3192 3192 Size: 5593 MluI A'CGCG_T Cuts at: 5469 5469 Size: 5593 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4707 4891 1305 Size: 3402 184 2007 Fragments arranged by size: 3402 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2816 10 Size: 54 1425 343 984 2787 Fragments arranged by size: 2787 1425 984 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2561 2600 2801 3551 3910 4069 Size: 648 843 552 39 201 750 359 159 Cuts at: 4069 518 Size: 2042 Fragments arranged by size: 2042 843 750 648 552 359 201 159 39 MspA1I CmG'CkG Cuts at: 1606 3009 3686 4834 5079 1606 Size: 1403 677 1148 245 2120 Fragments arranged by size: 2120 1403 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4102 Fragments arranged by size: 4102 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5593 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5593 NotI GC'GGCC_GC Cuts at: 3040 3040 Size: 5593 NruI TCG'CGA Cuts at: 2085 2085 Size: 5593 NsiI A_TGCA'T Cuts at: 2575 2575 Size: 5593 NspI r_CATG'y Cuts at: 1132 1229 2605 2951 4496 1132 Size: 97 1376 346 1545 2229 Fragments arranged by size: 2229 1545 1376 346 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5593 PciI A'CATG_T Cuts at: 2601 4492 2601 Size: 1891 3702 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5593 PmeI GTTT'AAAC Cuts at: 2897 2897 Size: 5593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3662 3694 5175 5207 3662 Size: 32 1481 32 4048 Fragments arranged by size: 4048 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5593 PsiI TTA'TAA Cuts at: 3163 3163 Size: 5593 PspOMI G'GGCC_C Cuts at: 2307 2307 Size: 5593 PstI C_TGCA'G Cuts at: 830 2530 2706 2945 830 Size: 1700 176 239 3478 Fragments arranged by size: 3478 1700 239 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5593 RsrII CG'GwC_CG Cuts at: 2067 2067 Size: 5593 SalI G'TCGA_C Cuts at: 1936 2935 1936 Size: 999 4594 SbfI CC_TGCA'GG Cuts at: 2945 2945 Size: 5593 ScaI AGT'ACT Cuts at: 2244 3841 5553 2244 Size: 1597 1712 2284 Fragments arranged by size: 2284 1712 1597 SfcI C'TryA_G Cuts at: 826 1045 2526 2702 2941 4076 4757 4948 826 Size: 219 1481 176 239 1135 681 191 1471 Fragments arranged by size: 1481 1471 1135 681 239 219 191 176 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5593 SmaI CCC'GGG Cuts at: 1872 2920 1872 Size: 1048 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2877 3697 4598 4860 820 Size: 197 926 934 820 901 262 1553 Fragments arranged by size: 1553 934 926 901 820 262 197 SnaBI TAC'GTA Cuts at: 493 3384 493 Size: 2891 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5593 SphI G_CATG'C Cuts at: 1132 2951 1132 Size: 1819 3774 SspI AAT'ATT Cuts at: 5 52 3517 5 Size: 47 3465 2081 Fragments arranged by size: 3465 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5593 StyI C'CwwG_G Cuts at: 513 1065 2004 2743 3032 513 Size: 552 939 739 289 3074 Fragments arranged by size: 3074 939 739 552 289 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 2420 2551 3620 3637 3790 3975 Size: 1255 633 207 131 1069 17 153 185 Cuts at: 3975 4420 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1069 633 445 207 185 153 131 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2242 3839 5551 Size: 274 80 33 51 1443 265 1597 1712 Cuts at: 5551 96 Size: 138 Fragments arranged by size: 1712 1597 1443 274 265 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5553 5585 5553 Size: 32 5561 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5593 XbaI T'CTAG_A Cuts at: 2929 2929 Size: 5593 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5593 XmaI C'CCGG_G Cuts at: 1870 2918 1870 Size: 1048 4545 XmnI GAAnn'nnTTC Cuts at: 2255 3722 2255 Size: 1467 4126 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3401 276 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI