Restriction map for
pFN21AE2707
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI ClaI
CpoI DraI DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 34 CCGC CCGC(-3/-1)
AcsI 49 AAATTC [GA]^AATT[CT]
AhdI 184 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 16 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 49 AAATTC [GA]^AATT[CT]
AseI 339 ATTAAT AT^TAAT
AsnI 339 ATTAAT AT^TAAT
AspEI 184 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 560 GGCACC G^G[CT][GA]CC
BbsI 132 GAAGAC GAAGAC(2/6)
BbvI 685 GCAGC GCAGC(8/12)
BfaI 410 CTAG C^TAG
BpuAI 132 GAAGAC GAAGAC(2/6)
BsmI 429 GAATGC GAATGC(1/-1)
BsrFI 149 GCCGGT [GA]^CCGG[CT]
BsrI 653 CCAGT ACTGG(1/-1)
BssSI 10 CACGAG CACGAG(-5/-1)
BstF5I 632 CATCC GGATG(2/0)
BstYI 17 AGATCC [GA]^GATC[CT]
Cfr10I 149 GCCGGT [GA]^CCGG[CT]
DraII 57 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 464 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 184 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 57 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 431 ATGCAT ATGCA^T
Fnu4HI 685 GCAGC GC^[ACGT]GC
FokI 632 CATCC GGATG(9/13)
HapII 150 CCGG C^CGG
HincII 192 GTCAAC GT[CT]^[GA]AC
HindII 192 GTCAAC GT[CT]^[GA]AC
HpaII 150 CCGG C^CGG
HphI 650 TCACC GGTGA(8/7)
ItaI 685 GCAGC GC^[ACGT]GC
MaeI 410 CTAG C^TAG
MflI 17 AGATCC [GA]^GATC[CT]
MspI 150 CCGG C^CGG
NsiI 431 ATGCAT ATGCA^T
PshBI 339 ATTAAT AT^TAAT
TaqI 29 TCGA T^CGA
TseI 685 GCAGC G^C[AT]GC
TthHB8I 29 TCGA T^CGA
XhoII 17 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 153 GTAC GT^AC
268 GTAC
AvaII 24 GGTCC G^G[AT]CC
448 GGACC
BsaJI 186 CCCAGG C^C[ACGT][ACGT]GG
187 CCAGGG
BsiYI 166 CCCAACAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
488 CCGAAACAAGG
BslI 166 CCCAACAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
488 CCGAAACAAGG
EarI 659 CTCTTC CTCTTC(1/4)
508 GAAGAG
HaeIII 58 GGCC GG^CC
164 GGCC
HinfI 346 GATTC G^A[ACGT]TC
701 GATTC
Ksp632I 659 CTCTTC CTCTTC(1/4)
508 GAAGAG
RsaI 153 GTAC GT^AC
268 GTAC
SfcI 137 CTACAG C^T[GA][CT]AG
217 CTACAG
TfiI 346 GATTC G^A[AT]TC
701 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AlwI BssKI BstNI Cfr13I
DdeI DpnII EcoRII MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIV Sau3AI Sau96I ScrFI SfaNI
Tru9I Tsp45I Tsp509I TspRI