################################## # NO CUT ENZYMES ( 205 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 137 AAATTC [GA]^AATT[CT] AlwI 105 GGATC GGATC(4/5) ApoI 137 AAATTC [GA]^AATT[CT] Asp700I 213 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 13 GGACC G^G[AT]CC BbsI 41 GTCTTC GAAGAC(2/6) BfaI 166 CTAG C^TAG BpmI 82 CTGGAG CTGGAG(16/14) BpuAI 41 GTCTTC GAAGAC(2/6) BsaJI 183 CCTTGG C^C[ACGT][ACGT]GG BsgI 87 GTGCAG GTGCAG(16/14) BsiYI 75 CCTACTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 75 CCTACTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 147 GTCTC GTCTC(1/5) BssKI 10 CCTGG ^CC[ACGT]GG BstAPI 72 GCACCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 10 CCTGG CC^[AT]GG Cac8I 240 GCTGGC GC[ACGT]^[ACGT]GC DpnII 106 GATC ^GATC EcoRII 10 CCTGG ^CC[AT]GG EcoT14I 183 CCTTGG C^C[AT][AT]GG HaeIII 243 GGCC GG^CC HinfI 253 GAGTC G^A[ACGT]TC MaeI 166 CTAG C^TAG MaeIII 25 GTTAC ^GT[ACGT]AC MboI 106 GATC ^GATC MboII 42 TCTTC GAAGA(8/7) MseI 68 TTAA T^TAA MvaI 10 CCTGG CC^[AT]GG NdeII 106 GATC ^GATC PleI 253 GAGTC GAGTC(4/5) PstI 111 CTGCAG CTGCA^G Sau3AI 106 GATC ^GATC ScrFI 10 CCTGG CC^[ACGT]GG SfcI 111 CTGCAG C^T[GA][CT]AG StyI 183 CCTTGG C^C[AT][AT]GG Tru9I 68 TTAA T^TAA Tsp509I 138 AATT ^AATT XmnI 213 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AluI 164 AGCT AG^CT 215 AGCT BbvI 113 GCAGC GCAGC(8/12) 162 GCAGC BsrI 127 CCAGT ACTGG(1/-1) 246 CCAGT Cfr13I 13 GGACC G^G[ACGT]CC 243 GGCCC DdeI 149 CTCAG C^T[ACGT]AG 158 CTGAG Fnu4HI 113 GCAGC GC^[ACGT]GC 162 GCAGC ItaI 113 GCAGC GC^[ACGT]GC 162 GCAGC NlaIV 6 GGAGCC GG[ACGT]^[ACGT]CC 13 GGACCC Sau96I 13 GGACC G^G[ACGT]CC 243 GGCCC TseI 113 GCAGC G^C[AT]GC 162 GCAGC TspRI 90 CAGTG CA[GC]TG[ACGT][ACGT]^ 109 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### MnlI MwoI NlaIII