################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BciVI BfaI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HindIII KasI KspI MaeI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 123 GGTACC G^GTACC AcsI 1123 AAATTT [GA]^AATT[CT] AflII 243 CTTAAG C^TTAAG AocI 535 CCTGAGG CC^T[ACGT]AGG ApoI 1123 AAATTT [GA]^AATT[CT] Asp700I 667 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 123 GGTACC G^GTACC AspHI 304 GAGCAC G[AT]GC[AT]^C BalI 631 TGGCCA TGG^CCA BanI 123 GGTACC G^G[CT][GA]CC BanII 1249 GAGCCC G[GA]GC[CT]^C BbsI 316 GAAGAC GAAGAC(2/6) BcgI 513 GCAGTATAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 495 TGATCA T^GATCA BcnI 1260 CCGGG CC^[GC]GG BfrI 243 CTTAAG C^TTAAG BpuAI 316 GAAGAC GAAGAC(2/6) BsaI 541 GAGACC GGTCTC(1/5) BsiHKAI 304 GAGCAC G[AT]GC[AT]^C BsmI 884 GCATTC GAATGC(1/-1) BspHI 806 TCATGA T^CATGA BsrBI 1368 GAGCGG CCGCTC(-3/-3) BssSI 743 CTCGTG CACGAG(-5/-1) BstEII 619 GGTTACC G^GT[ACGT]ACC BstF5I 585 CATCC GGATG(2/0) BstPI 619 GGTTACC G^GT[ACGT]ACC Bsu36I 535 CCTGAGG CC^T[ACGT]AGG DraI 1126 TTTAAA TTT^AAA DsaI 1412 CCGTGG C^C[GA][CT]GG EaeI 631 TGGCCA [CT]^GGCC[GA] Eco81I 535 CCTGAGG CC^T[ACGT]AGG EcoO65I 619 GGTTACC G^GT[ACGT]ACC EcoT14I 1148 CCTTGG C^C[AT][AT]GG FauI 1370 GCGGG CCCGC(4/6) FbaI 495 TGATCA T^GATCA FokI 585 CATCC GGATG(9/13) HaeII 1416 GGCGCT [GA]GCGC^[CT] HapII 1260 CCGG C^CGG HpaI 898 GTTAAC GTT^AAC HpaII 1260 CCGG C^CGG KpnI 123 GGTACC GGTAC^C MaeII 721 ACGT A^CGT MluNI 631 TGGCCA TGG^CCA MscI 631 TGGCCA TGG^CCA MslI 504 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1260 CCGG C^CGG NciI 1260 CCGGG CC^[GC]GG NspI 764 ACATGC [GA]CATG^[CT] RcaI 806 TCATGA T^CATGA SapI 1246 GAAGAGC GCTCTTC(1/4) SspI 668 AATATT AAT^ATT StuI 1145 AGGCCT AGG^CCT StyI 1148 CCTTGG C^C[AT][AT]GG XmnI 667 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 444 GCGG CCGC(-3/-1) 1370 GCGG AfaI 124 GTAC GT^AC 711 GTAC BseRI 118 GAGGAG GAGGAG(10/8) 538 GAGGAG BsgI 209 CTGCAC GTGCAG(16/14) 511 GTGCAG BsmAI 25 GAGAC GTCTC(1/5) 541 GAGAC CfoI 816 GCGC GCG^C 1417 GCGC DraII 1110 AGGCCCT [GA]G^G[ACGT]CC[CT] 1222 GGGGCCT EarI 1018 GAAGAG CTCTTC(1/4) 1246 GAAGAG EcoNI 655 CCTGACTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1269 CCTGACCCAGG EcoO109I 1110 AGGCCCT [GA]G^G[ACGT]CC[CT] 1222 GGGGCCT HhaI 816 GCGC GCG^C 1417 GCGC HinP1I 816 GCGC G^CGC 1417 GCGC HincII 760 GTCAAC GT[CT]^[GA]AC 898 GTTAAC HindII 760 GTCAAC GT[CT]^[GA]AC 898 GTTAAC HphI 160 GGTGA GGTGA(8/7) 239 TCACC Ksp632I 1018 GAAGAG CTCTTC(1/4) 1246 GAAGAG PleI 236 GACTC GAGTC(4/5) 1076 GAGTC PstI 828 CTGCAG CTGCA^G 1378 CTGCAG RsaI 124 GTAC GT^AC 711 GTAC SfcI 828 CTGCAG C^T[GA][CT]AG 1378 CTGCAG Tsp45I 718 GTGAC ^GT[GC]AC 1316 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AluI AlwI AlwNI AvaII BamHI BbvI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HinfI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PflMI Sau3AI Sau96I ScrFI SmlI TfiI Tru9I TseI Tsp509I TspRI Van91I XhoII