(Circular) (Six-base) MAPSORT of: pFN21AE5644.seq Check: 7467 from: 1 to: 6136 pFN21AE5644 6136 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3946 278 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3479 1937 Size: 1542 4594 Acc65I G'GTAC_C Cuts at: 2128 3457 2128 Size: 1329 4807 AclI AA'CG_TT Cuts at: 1563 4263 4636 1563 Size: 2700 373 3063 Fragments arranged by size: 3063 2700 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4199 5583 5851 1632 Size: 2567 1384 268 1917 Fragments arranged by size: 2567 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2248 820 Size: 197 1231 4708 Fragments arranged by size: 4708 1231 197 AflIII A'CryG_T Cuts at: 1203 5035 6012 1203 Size: 3832 977 1327 Fragments arranged by size: 3832 1327 977 AgeI A'CCGG_T Cuts at: 4967 4967 Size: 6136 AhdI GACnn_n'nnGTC Cuts at: 4865 4865 Size: 6136 AleI CACnn'nnGTG Cuts at: 1166 2513 1166 Size: 1347 4789 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6136 AlwNI CAG_nnn'CTG Cuts at: 2213 2791 2845 3879 5451 2213 Size: 578 54 1034 1572 2898 Fragments arranged by size: 2898 1572 1034 578 54 ApaLI G'TGCA_C Cuts at: 4193 5349 4193 Size: 1156 4980 ApoI r'AATT_y Cuts at: 1493 3128 3445 3671 3823 5932 1493 Size: 1635 317 226 152 2109 1697 Fragments arranged by size: 2109 1697 1635 317 226 152 AseI AT'TA_AT Cuts at: 160 1032 4690 160 Size: 872 3658 1606 Fragments arranged by size: 3658 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 AvaI C'yCGr_G Cuts at: 1870 1943 3461 1870 Size: 73 1518 4545 Fragments arranged by size: 4545 1518 73 BamHI G'GATC_C Cuts at: 2597 2757 2993 3466 3833 2597 Size: 160 236 473 367 4900 Fragments arranged by size: 4900 473 367 236 160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 3457 4912 618 Size: 325 217 627 341 1329 1455 1842 Fragments arranged by size: 1842 1455 1329 627 341 325 217 BanII G_rGCy'C Cuts at: 1391 3258 3455 1391 Size: 1867 197 4072 Fragments arranged by size: 4072 1867 197 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2328 928 Size: 971 429 4736 Fragments arranged by size: 4736 971 429 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2507 2541 4327 4361 1903 Size: 34 570 34 1786 34 3678 Fragments arranged by size: 3678 1786 570 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4028 5238 4028 Size: 1210 4926 BclI T'GATC_A Cuts at: 1548 2500 1548 Size: 952 5184 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4747 136 Size: 107 122 71 631 605 3075 1525 Fragments arranged by size: 3075 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6131 6131 Size: 6136 BlpI GC'TnA_GC Cuts at: 3553 3553 Size: 6136 Bme1580I G_kGCm'C Cuts at: 1790 2044 4197 5353 1790 Size: 254 2153 1156 2573 Fragments arranged by size: 2573 2153 1156 254 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6136 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2756 2855 3867 Size: 416 53 83 399 24 1339 99 1012 Cuts at: 3867 4825 442 Size: 958 1753 Fragments arranged by size: 1753 1339 1012 958 416 399 99 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6136 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2819 2941 3301 3353 4796 1239 Size: 1580 122 360 52 1443 2579 Fragments arranged by size: 2579 1580 1443 360 122 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2124 2256 2280 2582 2853 3323 4261 5126 5424 Size: 132 24 302 271 470 938 865 298 Cuts at: 5424 2124 Size: 2836 Fragments arranged by size: 2836 938 865 470 302 298 271 132 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 4799 882 Size: 617 1041 2259 2219 Fragments arranged by size: 2259 2219 1041 617 BsaAI yAC'GTr Cuts at: 493 1204 3927 493 Size: 711 2723 2702 Fragments arranged by size: 2723 2702 711 BsaBI GATnn'nnATC Cuts at: 6130 6130 Size: 6136 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3943 4325 4960 275 Size: 53 83 186 936 2410 382 635 1451 Fragments arranged by size: 2410 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4569 4967 5241 5388 5799 4569 Size: 398 274 147 411 4906 Fragments arranged by size: 4906 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2112 2142 2727 2757 3299 Size: 30 165 30 709 30 585 30 542 Cuts at: 3299 3329 1178 Size: 30 3985 Fragments arranged by size: 3985 709 585 542 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2558 1186 Size: 952 420 4764 Fragments arranged by size: 4764 952 420 BseYI C'CCAG_C Cuts at: 2043 3364 5339 5835 2043 Size: 1321 1975 496 2344 Fragments arranged by size: 2344 1975 1321 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2199 2537 1163 Size: 573 463 338 4762 Fragments arranged by size: 4762 573 463 338 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3586 4347 5375 1540 Size: 275 186 1585 761 1028 2301 Fragments arranged by size: 2301 1585 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 4197 4282 5353 2313 Size: 1884 85 1071 3096 Fragments arranged by size: 3096 1884 1071 85 BsmI GAATG_Cn' Cuts at: 2889 3647 3740 2889 Size: 758 93 5285 Fragments arranged by size: 5285 758 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2313 3258 3455 4197 4282 5353 Size: 399 254 269 945 197 742 85 1071 Cuts at: 5353 1391 Size: 2174 Fragments arranged by size: 2174 1071 945 742 399 269 254 197 85 BspEI T'CCGG_A Cuts at: 5799 5799 Size: 6136 BspHI T'CATG_A Cuts at: 2811 4025 5755 2811 Size: 1214 1730 3192 Fragments arranged by size: 3192 1730 1214 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3491 844 Size: 2647 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3375 4023 1394 Size: 429 1552 648 3507 Fragments arranged by size: 3507 1552 648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4631 4805 65 Size: 1183 3383 174 1396 Fragments arranged by size: 3383 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4780 4967 1700 Size: 110 2970 187 2869 Fragments arranged by size: 2970 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6136 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6136 BssSI C'ACGA_G Cuts at: 2748 4196 5208 2748 Size: 1448 1012 3676 Fragments arranged by size: 3676 1448 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6136 BstBI TT'CG_AA Cuts at: 4940 4940 Size: 6136 BstEII G'GTnAC_C Cuts at: 1184 2624 1184 Size: 1440 4696 BstXI CCAn_nnnn'nTGG Cuts at: 2490 3287 3325 2490 Size: 797 38 5301 Fragments arranged by size: 5301 797 38 BstYI r'GATC_y Cuts at: 1972 2009 2209 2597 2757 2936 2993 3466 3833 Size: 37 200 388 160 179 57 473 367 Cuts at: 3833 4218 4235 5687 5773 5785 6131 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 473 388 385 367 346 200 179 160 86 57 37 17 12 Bsu36I CC'TnA_GG Cuts at: 2541 2541 Size: 6136 BtgI C'CryG_G Cuts at: 513 1065 2004 3417 513 Size: 552 939 1413 3232 Fragments arranged by size: 3232 1413 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5397 Fragments arranged by size: 5397 683 56 BtsI GCAGTG_nn' Cuts at: 3652 4446 4466 3652 Size: 794 20 5322 Fragments arranged by size: 5322 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6136 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6101 DraI TTT'AAA Cuts at: 3133 3440 3133 Size: 307 5829 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5143 809 Size: 507 3827 1802 Fragments arranged by size: 3827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2636 3583 4472 Size: 54 1425 50 275 18 806 947 889 Cuts at: 4472 6101 8 Size: 1629 43 Fragments arranged by size: 1629 1425 947 889 806 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 1537 Size: 275 1771 4090 Fragments arranged by size: 4090 1771 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3018 3246 4066 1353 Size: 1665 228 820 3423 Fragments arranged by size: 3423 1665 820 228 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4723 5097 5243 387 Size: 1064 138 3134 374 146 1280 Fragments arranged by size: 3134 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2819 2941 3301 3353 4199 4796 5583 Size: 393 1187 122 360 52 846 597 787 Cuts at: 5583 5851 1239 Size: 268 1524 Fragments arranged by size: 1524 1187 846 787 597 393 360 268 122 52 EcoNI CCTnn'n_nnAGG Cuts at: 2664 3278 2664 Size: 614 5522 EcoO109I rG'GnC_Cy Cuts at: 3116 3228 3116 Size: 112 6024 EcoRI G'AATT_C Cuts at: 3445 3823 3445 Size: 378 5758 FspI TGC'GCA Cuts at: 4642 4642 Size: 6136 HaeII r_GCGC'y Cuts at: 1678 3425 5283 1678 Size: 1747 1858 2531 Fragments arranged by size: 2531 1858 1747 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 2589 2621 2851 Size: 32 135 32 905 32 80 32 230 Cuts at: 2851 2883 3299 3331 4777 4809 4851 4883 1373 Size: 32 416 32 1446 32 42 32 2626 Fragments arranged by size: 2626 1446 905 416 230 135 80 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2767 2905 3480 3726 669 Size: 988 281 829 138 575 246 3079 Fragments arranged by size: 3079 988 829 575 281 246 138 HindIII A'AGCT_T Cuts at: 748 748 Size: 6136 HpaI GTT'AAC Cuts at: 2905 3726 2905 Size: 821 5315 KpnI G_GTAC'C Cuts at: 2132 3461 2132 Size: 1329 4807 MfeI C'AATT_G Cuts at: 3735 3735 Size: 6136 MluI A'CGCG_T Cuts at: 6012 6012 Size: 6136 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3009 5250 5434 1305 Size: 1704 2241 184 2007 Fragments arranged by size: 2241 2007 1704 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2638 10 Size: 54 1425 343 806 3508 Fragments arranged by size: 3508 1425 806 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 4094 4453 4612 518 Size: 648 1347 1581 359 159 2042 Fragments arranged by size: 2042 1581 1347 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3552 4229 5377 5622 1606 Size: 1946 677 1148 245 2120 Fragments arranged by size: 2120 1946 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4645 Fragments arranged by size: 4645 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6136 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6136 NotI GC'GGCC_GC Cuts at: 3583 3583 Size: 6136 NspI r_CATG'y Cuts at: 1132 1229 2773 3494 5039 1132 Size: 97 1544 721 1545 2229 Fragments arranged by size: 2229 1545 1544 721 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6136 PciI A'CATG_T Cuts at: 5035 5035 Size: 6136 PflMI CCAn_nnn'nTGG Cuts at: 1108 2435 2607 2845 1108 Size: 1327 172 238 4399 Fragments arranged by size: 4399 1327 238 172 PfoI T'CCnGG_A Cuts at: 2784 2784 Size: 6136 PmeI GTTT'AAAC Cuts at: 3440 3440 Size: 6136 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 5718 5750 4205 Size: 32 1481 32 4591 Fragments arranged by size: 4591 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6136 PsiI TTA'TAA Cuts at: 3706 3706 Size: 6136 PstI C_TGCA'G Cuts at: 830 2837 3387 3488 830 Size: 2007 550 101 3478 Fragments arranged by size: 3478 2007 550 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6136 SalI G'TCGA_C Cuts at: 1936 3478 1936 Size: 1542 4594 SapI GCTCTTCn'nnn_ Cuts at: 3246 3246 Size: 6136 SbfI CC_TGCA'GG Cuts at: 3488 3488 Size: 6136 ScaI AGT'ACT Cuts at: 4384 6096 4384 Size: 1712 4424 SfcI C'TryA_G Cuts at: 826 1045 2833 3383 3484 4619 5300 5491 826 Size: 219 1788 550 101 1135 681 191 1471 Fragments arranged by size: 1788 1471 1135 681 550 219 191 101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6136 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6136 SmaI CCC'GGG Cuts at: 1872 3463 1872 Size: 1591 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2139 2248 2271 2295 2561 2868 Size: 197 926 196 109 23 24 266 307 Cuts at: 2868 3302 4240 5141 5403 820 Size: 434 938 901 262 1553 Fragments arranged by size: 1553 938 926 901 434 307 266 262 197 196 109 24 23 SnaBI TAC'GTA Cuts at: 493 3927 493 Size: 3434 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6136 SphI G_CATG'C Cuts at: 1132 3494 1132 Size: 2362 3774 SspI AAT'ATT Cuts at: 5 52 2675 4060 5 Size: 47 2623 1385 2081 Fragments arranged by size: 2623 2081 1385 47 StuI AGG'CCT Cuts at: 1517 3152 1517 Size: 1635 4501 StyI C'CwwG_G Cuts at: 513 1065 2004 3153 3575 513 Size: 552 939 1149 422 3074 Fragments arranged by size: 3074 1149 939 552 422 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2340 4163 4180 4333 4518 4963 325 Size: 1255 760 1823 17 153 185 445 1498 Fragments arranged by size: 1823 1498 1255 760 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4382 6094 96 Size: 274 80 33 51 1443 2405 1712 138 Fragments arranged by size: 2405 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6096 6128 6096 Size: 32 6104 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6136 XbaI T'CTAG_A Cuts at: 3472 3472 Size: 6136 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6136 XmaI C'CCGG_G Cuts at: 1870 3461 1870 Size: 1591 4545 XmnI GAAnn'nnTTC Cuts at: 2676 4265 2676 Size: 1589 4547 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3944 276 Size: 53 83 186 936 2410 2468 Fragments arranged by size: 2468 2410 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI