(Linear) (Six-base) MAPSORT of: KIEE5644.seq Check: 4066 from: 1 to: 1431 KIEE5644.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. Acc65I G'GTAC_C Cuts at: 0 123 1431 Size: 123 1308 AflII C'TTAA_G Cuts at: 0 243 1431 Size: 243 1188 AleI CACnn'nnGTG Cuts at: 0 508 1431 Size: 508 923 AlwNI CAG_nnn'CTG Cuts at: 0 208 786 840 1431 Size: 208 578 54 591 Fragments arranged by size: 591 578 208 54 ApoI r'AATT_y Cuts at: 0 1123 1431 Size: 1123 308 BamHI G'GATC_C Cuts at: 0 592 752 988 1431 Size: 592 160 236 443 Fragments arranged by size: 592 443 236 160 BanI G'GyrC_C Cuts at: 0 123 1431 Size: 123 1308 BanII G_rGCy'C Cuts at: 0 1253 1431 Size: 1253 178 BbsI GAAGACnn'nnnn_ Cuts at: 0 323 1431 Size: 323 1108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 502 536 1431 Size: 502 34 895 Fragments arranged by size: 895 502 34 BclI T'GATC_A Cuts at: 0 495 1431 Size: 495 936 Bme1580I G_kGCm'C Cuts at: 0 39 1431 Size: 39 1392 BmrI ACTGGGnnnn_n' Cuts at: 0 751 850 1431 Size: 751 99 581 Fragments arranged by size: 751 581 99 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 814 936 1296 1348 1431 Size: 814 122 360 52 83 Fragments arranged by size: 814 360 122 83 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 251 275 577 848 1318 1431 Size: 119 132 24 302 271 470 113 Fragments arranged by size: 470 302 271 132 119 113 24 BsaI GGTCTCn'nnnn_ Cuts at: 0 535 1431 Size: 535 896 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 107 137 722 752 1294 1324 1431 Size: 107 30 585 30 542 30 107 Fragments arranged by size: 585 542 107 107 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 133 553 1431 Size: 133 420 878 Fragments arranged by size: 878 420 133 BseYI C'CCAG_C Cuts at: 0 38 1359 1431 Size: 38 1321 72 Fragments arranged by size: 1321 72 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 194 532 1431 Size: 194 338 899 Fragments arranged by size: 899 338 194 BsiHKAI G_wGCw'C Cuts at: 0 308 1431 Size: 308 1123 BsmI GAATG_Cn' Cuts at: 0 884 1431 Size: 884 547 Bsp1286I G_dGCh'C Cuts at: 0 39 308 1253 1431 Size: 39 269 945 178 Fragments arranged by size: 945 269 178 39 BspHI T'CATG_A Cuts at: 0 806 1431 Size: 806 625 BsrBI CCG'CTC Cuts at: 0 1370 1431 Size: 1370 61 BssSI C'ACGA_G Cuts at: 0 743 1431 Size: 743 688 BstEII G'GTnAC_C Cuts at: 0 619 1431 Size: 619 812 BstXI CCAn_nnnn'nTGG Cuts at: 0 485 1282 1320 1431 Size: 485 797 38 111 Fragments arranged by size: 797 485 111 38 BstYI r'GATC_y Cuts at: 0 4 204 592 752 931 988 1431 Size: 4 200 388 160 179 57 443 Fragments arranged by size: 443 388 200 179 160 57 4 Bsu36I CC'TnA_GG Cuts at: 0 536 1431 Size: 536 895 BtgI C'CryG_G Cuts at: 0 1412 1431 Size: 1412 19 DraI TTT'AAA Cuts at: 0 1128 1431 Size: 1128 303 EaeI y'GGCC_r Cuts at: 0 631 1431 Size: 631 800 EarI CTCTTCn'nnn_ Cuts at: 0 1013 1241 1431 Size: 1013 228 190 Fragments arranged by size: 1013 228 190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 814 936 1296 1348 1431 Size: 814 122 360 52 83 Fragments arranged by size: 814 360 122 83 52 EcoNI CCTnn'n_nnAGG Cuts at: 0 659 1273 1431 Size: 659 614 158 Fragments arranged by size: 659 614 158 EcoO109I rG'GnC_Cy Cuts at: 0 1111 1223 1431 Size: 1111 112 208 Fragments arranged by size: 1111 208 112 HaeII r_GCGC'y Cuts at: 0 1420 1431 Size: 1420 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 472 504 584 616 846 878 1294 1326 Size: 472 32 80 32 230 32 416 32 Cuts at: 1326 1431 Size: 105 Fragments arranged by size: 472 416 230 105 80 32 32 32 32 HincII GTy'rAC Cuts at: 0 762 900 1431 Size: 762 138 531 Fragments arranged by size: 762 531 138 HpaI GTT'AAC Cuts at: 0 900 1431 Size: 900 531 KpnI G_GTAC'C Cuts at: 0 127 1431 Size: 127 1304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1004 1431 Size: 1004 427 MscI TGG'CCA Cuts at: 0 633 1431 Size: 633 798 MslI CAynn'nnrTG Cuts at: 0 508 1431 Size: 508 923 NspI r_CATG'y Cuts at: 0 768 1431 Size: 768 663 PflMI CCAn_nnn'nTGG Cuts at: 0 430 602 840 1431 Size: 430 172 238 591 Fragments arranged by size: 591 430 238 172 PfoI T'CCnGG_A Cuts at: 0 779 1431 Size: 779 652 PstI C_TGCA'G Cuts at: 0 832 1382 1431 Size: 832 550 49 Fragments arranged by size: 832 550 49 SapI GCTCTTCn'nnn_ Cuts at: 0 1241 1431 Size: 1241 190 SfcI C'TryA_G Cuts at: 0 828 1378 1431 Size: 828 550 53 Fragments arranged by size: 828 550 53 SmlI C'TyrA_G Cuts at: 0 134 243 266 290 556 863 1297 1431 Size: 134 109 23 24 266 307 434 134 Fragments arranged by size: 434 307 266 134 134 109 24 23 SspI AAT'ATT Cuts at: 0 670 1431 Size: 670 761 StuI AGG'CCT Cuts at: 0 1147 1431 Size: 1147 284 StyI C'CwwG_G Cuts at: 0 1148 1431 Size: 1148 283 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 335 1431 Size: 335 1096 XmnI GAAnn'nnTTC Cuts at: 0 671 1431 Size: 671 760 Enzymes that do cut: Acc65I AflII AleI AlwNI ApoI BamHI BanI BanII BbsI BcgI BclI Bme1580I BmrI BpmI BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI BssSI BstEII BstXI BstYI Bsu36I BtgI DraI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HpaI KpnI MmeI MscI MslI NspI PflMI PfoI PstI SapI SfcI SmlI SspI StuI StyI TaqII XmnI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII KasI MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI