################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspI AviII AvrII BbrPI BbvCI BciVI BcnI BfrI BglI BglII BlnI BsaAI BsaI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HapII HgaI HindIII HpaI HpaII KpnI KspI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AcyI 1239 GGCGCC G[GA]^CG[CT]C AseI 856 ATTAAT AT^TAAT AsnI 856 ATTAAT AT^TAAT Asp700I 1384 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 223 CTCGAG C^[CT]CG[GA]G AvaII 297 GGTCC G^G[AT]CC BalI 556 TGGCCA TGG^CCA BamHI 997 GGATCC G^GATCC BanI 1239 GGCGCC G^G[CT][GA]CC BbeI 1239 GGCGCC GGCGC^C BcgI 744 CGAACAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1058 TGATCA T^GATCA BlpI 724 GCTGAGC GC^T[ACGT]AGC BpmI 337 CTGGAG CTGGAG(16/14) Bpu1102I 724 GCTGAGC GC^T[ACGT]AGC BsaBI 238 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1239 GGCGCC G[GA]^CG[CT]C BsgI 83 CTGCAC GTGCAG(16/14) BsmAI 1341 GAGAC GTCTC(1/5) BsmBI 1341 GAGACG CGTCTC(1/5) BsmFI 298 GTCCC GGGAC(10/14) BsoBI 223 CTCGAG C^[CT]CG[GA]G BstBI 542 TTCGAA TT^CGAA BstYI 997 GGATCC [GA]^GATC[CT] CelII 724 GCTGAGC GC^T[ACGT]AGC CfoI 1240 GCGC GCG^C DraII 227 AGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 964 GACATAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 556 TGGCCA [CT]^GGCC[GA] EcoO109I 227 AGGGCCT [GA]G^G[ACGT]CC[CT] FbaI 1058 TGATCA T^GATCA HaeII 1239 GGCGCC [GA]GCGC^[CT] HhaI 1240 GCGC GCG^C Hin1I 1239 GGCGCC G[GA]^CG[CT]C HinP1I 1240 GCGC G^CGC HincII 385 GTCAAC GT[CT]^[GA]AC HindII 385 GTCAAC GT[CT]^[GA]AC KasI 1239 GGCGCC G^GCGCC MamI 238 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 997 GGATCC [GA]^GATC[CT] MluNI 556 TGGCCA TGG^CCA MscI 556 TGGCCA TGG^CCA NarI 1239 GGCGCC GG^CGCC NspI 389 ACATGC [GA]CATG^[CT] NspV 542 TTCGAA TT^CGAA PaeR7I 223 CTCGAG C^TCGAG PshBI 856 ATTAAT AT^TAAT PstI 1401 CTGCAG CTGCA^G SapI 1384 GAAGAGC GCTCTTC(1/4) SexAI 293 ACCTGGT A^CC[AT]GGT SfcI 1401 CTGCAG C^T[GA][CT]AG SfoI 1239 GGCGCC GGC^GCC SfuI 542 TTCGAA TT^CGAA XbaI 1303 TCTAGA T^CTAGA XcmI 759 CCAAGTGAATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 223 CTCGAG C^TCGAG XhoII 997 GGATCC [GA]^GATC[CT] XmnI 1384 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 998 GATCC GGATC(4/5) 997 GGATC BanII 1015 GAGCCC G[GA]GC[CT]^C 1141 GAGCCC BbvI 1403 GCAGC GCAGC(8/12) 141 GCTGC BfaI 685 CTAG C^TAG 1304 CTAG Bsp1407I 303 TGTACA T^GTACA 317 TGTACA BsrGI 303 TGTACA T^GTACA 317 TGTACA Cac8I 1242 GCCCGC GC[ACGT]^[ACGT]GC 1274 GCAAGC DsaI 843 CCATGG C^C[GA][CT]GG 1145 CCATGG EcoT14I 843 CCATGG C^C[AT][AT]GG 1145 CCATGG FauI 1243 CCCGC CCCGC(4/6) 1259 GCGGG MaeI 685 CTAG C^TAG 1304 CTAG MaeII 1007 ACGT A^CGT 1344 ACGT MaeIII 668 GTGAC ^GT[ACGT]AC 1358 GTGAC MslI 619 CACCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 838 CACAACCATG MwoI 17 GCGACCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 715 GCTCAAAAGGC NcoI 843 CCATGG C^CATGG 1145 CCATGG SspBI 303 TGTACA T^GTACA 317 TGTACA StyI 843 CCATGG C^C[AT][AT]GG 1145 CCATGG TaqI 224 TCGA T^CGA 543 TCGA TseI 141 GCTGC G^C[AT]GC 1403 GCAGC Tsp45I 668 GTGAC ^GT[GC]AC 1358 GTGAC TthHB8I 224 TCGA T^CGA 543 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AspHI BbsI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TfiI Tru9I Tsp509I TspRI