(Circular) (Six-base) MAPSORT of: pFN21AE5645.seq Check: 3633 from: 1 to: 6145 pFN21AE5645 6145 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2000 2000 Size: 6145 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3018 3380 4208 5592 5860 1632 Size: 1386 362 828 1384 268 1917 Fragments arranged by size: 1917 1386 1384 828 362 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 5044 6021 1203 Size: 3841 977 1327 Fragments arranged by size: 3841 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 2628 1166 Size: 1462 4683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2363 2395 2363 Size: 32 6113 AlwNI CAG_nnn'CTG Cuts at: 3888 5460 3888 Size: 1572 4573 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2862 4699 160 Size: 872 1830 1837 1606 Fragments arranged by size: 1837 1830 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2228 3470 1870 Size: 73 285 1242 4545 Fragments arranged by size: 4545 1242 285 73 BamHI G'GATC_C Cuts at: 3002 3475 3842 3002 Size: 473 367 5305 Fragments arranged by size: 5305 473 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3244 3466 4921 618 Size: 325 217 627 1457 222 1455 1842 Fragments arranged by size: 1842 1457 1455 627 325 222 217 BanII G_rGCy'C Cuts at: 1391 3024 3150 3464 1391 Size: 1633 126 314 4072 Fragments arranged by size: 4072 1633 314 126 BbeI G_GCGC'C Cuts at: 3248 3248 Size: 6145 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 2354 3025 928 Size: 971 231 224 671 4048 Fragments arranged by size: 4048 971 671 231 224 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2738 2772 4336 4370 1903 Size: 34 801 34 1564 34 3678 Fragments arranged by size: 3678 1564 801 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 3063 1548 Size: 1515 4630 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4756 136 Size: 107 122 71 631 605 3084 1525 Fragments arranged by size: 3084 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 2730 3562 2730 Size: 832 5313 Bme1580I G_kGCm'C Cuts at: 1790 3271 4206 5362 1790 Size: 1481 935 1156 2573 Fragments arranged by size: 2573 1481 1156 935 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3876 4834 442 Size: 416 53 83 399 24 2459 958 1753 Fragments arranged by size: 2459 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 4805 1239 Size: 1124 2442 2579 Fragments arranged by size: 2579 2442 1124 Bpu10I CC'TnA_GC Cuts at: 2027 2027 Size: 6145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2265 3244 4270 5135 5433 2265 Size: 979 1026 865 298 2977 Fragments arranged by size: 2977 1026 979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3936 493 Size: 711 2732 2702 Fragments arranged by size: 2732 2702 711 BsaBI GATnn'nnATC Cuts at: 2247 6139 2247 Size: 3892 2253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3245 3952 4334 4969 Size: 53 83 186 936 1712 707 382 635 Cuts at: 4969 275 Size: 1451 Fragments arranged by size: 1712 1451 936 707 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4578 4976 5250 5397 5808 4578 Size: 398 274 147 411 4915 Fragments arranged by size: 4915 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2483 2513 1178 Size: 30 165 30 1080 30 4810 Fragments arranged by size: 4810 1080 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2507 3251 3254 1186 Size: 1321 744 3 4077 Fragments arranged by size: 4077 1321 744 3 BseYI C'CCAG_C Cuts at: 3335 5348 5844 3335 Size: 2013 496 3636 Fragments arranged by size: 3636 2013 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2073 1163 Size: 573 337 5235 Fragments arranged by size: 5235 573 337 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3595 4356 5384 1540 Size: 275 186 1594 761 1028 2301 Fragments arranged by size: 2301 1594 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2033 2625 2722 4206 4291 5362 2033 Size: 592 97 1484 85 1071 2816 Fragments arranged by size: 2816 1484 1071 592 97 85 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 BsmBI CGTCTCn'nnnn_ Cuts at: 3340 3340 Size: 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2625 2722 3024 3150 3271 3464 Size: 399 243 592 97 302 126 121 193 Cuts at: 3464 4206 4291 5362 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 592 399 302 243 193 126 121 97 85 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2000 2367 3413 3500 844 Size: 1156 367 1046 87 3489 Fragments arranged by size: 3489 1156 1046 367 87 BsrBI CCG'CTC Cuts at: 1394 1823 4032 1394 Size: 429 2209 3507 Fragments arranged by size: 3507 2209 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4640 4814 65 Size: 1183 3392 174 1396 Fragments arranged by size: 3392 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4789 4976 1700 Size: 110 2979 187 2869 Fragments arranged by size: 2979 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2308 2322 96 Size: 2212 14 3919 Fragments arranged by size: 3919 2212 14 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 2548 4949 2548 Size: 2401 3744 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstYI r'GATC_y Cuts at: 1972 3002 3475 3842 4227 4244 5696 5782 5794 Size: 1030 473 367 385 17 1452 86 12 Cuts at: 5794 6140 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1030 473 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2848 3150 513 Size: 552 939 844 302 3508 Fragments arranged by size: 3508 939 844 552 302 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2400 487 Size: 683 56 1174 4232 Fragments arranged by size: 4232 1174 683 56 BtsI GCAGTG_nn' Cuts at: 2992 3661 4455 4475 2992 Size: 669 794 20 4662 Fragments arranged by size: 4662 794 669 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2995 3030 529 Size: 35 2431 35 3644 Fragments arranged by size: 3644 2431 35 35 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2975 5152 809 Size: 507 1659 2177 1802 Fragments arranged by size: 2177 1802 1659 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2561 3592 4481 Size: 54 1425 50 275 18 731 1031 889 Cuts at: 4481 6110 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1031 889 731 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3592 1537 Size: 275 1780 4090 Fragments arranged by size: 4090 1780 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 3338 3384 3418 4075 1353 Size: 1125 860 46 34 657 3423 Fragments arranged by size: 3423 1125 860 657 46 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 3018 3380 4208 4805 5592 5860 Size: 393 731 655 362 828 597 787 268 Cuts at: 5860 1239 Size: 1524 Fragments arranged by size: 1524 828 787 731 655 597 393 362 268 EcoO109I rG'GnC_Cy Cuts at: 2233 2233 Size: 6145 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2574 2574 Size: 6145 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 3248 5292 1678 Size: 1570 2044 2531 Fragments arranged by size: 2531 2044 1570 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2065 2097 2483 2515 4786 Size: 32 135 32 493 32 386 32 2271 Cuts at: 4786 4818 4860 4892 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2271 493 386 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2392 3489 3735 669 Size: 988 281 454 1097 246 3079 Fragments arranged by size: 3079 1097 988 454 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KasI G'GCGC_C Cuts at: 3244 3244 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2533 5259 5443 1305 Size: 1228 2726 184 2007 Fragments arranged by size: 2726 2007 1228 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2563 10 Size: 54 1425 343 731 3592 Fragments arranged by size: 3592 1425 731 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2628 2847 4103 4462 4621 518 Size: 648 1462 219 1256 359 159 2042 Fragments arranged by size: 2042 1462 1256 648 359 219 159 MspA1I CmG'CkG Cuts at: 1606 3561 4238 5386 5631 1606 Size: 1955 677 1148 245 2120 Fragments arranged by size: 2120 1955 1148 677 245 NarI GG'CG_CC Cuts at: 3245 3245 Size: 6145 NcoI C'CATG_G Cuts at: 513 1065 2004 2848 3150 513 Size: 552 939 844 302 3508 Fragments arranged by size: 3508 939 844 552 302 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 2398 3503 5048 1132 Size: 97 1169 1105 1545 2229 Fragments arranged by size: 2229 1545 1169 1105 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6145 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 2427 3715 2427 Size: 1288 4857 PspXI vC'TCGA_Gb Cuts at: 2228 2228 Size: 6145 PstI C_TGCA'G Cuts at: 830 3410 3497 830 Size: 2580 87 3478 Fragments arranged by size: 3478 2580 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SapI GCTCTTCn'nnn_ Cuts at: 3384 3384 Size: 6145 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SexAI A'CCwGG_T Cuts at: 2298 2298 Size: 6145 SfcI C'TryA_G Cuts at: 826 1045 3406 3493 4628 5309 5500 826 Size: 219 2361 87 1135 681 191 1471 Fragments arranged by size: 2361 1471 1135 681 219 191 87 SfoI GGC'GCC Cuts at: 3246 3246 Size: 6145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 3472 1872 Size: 1600 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 2280 3259 4249 5150 5412 Size: 197 926 285 52 979 990 901 262 Cuts at: 5412 820 Size: 1553 Fragments arranged by size: 1553 990 979 926 901 285 262 197 52 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2004 2848 3150 3584 513 Size: 552 939 844 302 434 3074 Fragments arranged by size: 3074 939 844 552 434 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4172 4189 4342 4527 4972 325 Size: 1255 2592 17 153 185 445 1498 Fragments arranged by size: 2592 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2308 2322 3314 Size: 274 80 33 51 1443 331 14 992 Cuts at: 3314 4391 6103 96 Size: 1077 1712 138 Fragments arranged by size: 1712 1443 1077 992 331 274 138 80 51 33 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6105 6137 6105 Size: 32 6113 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3308 3481 3308 Size: 173 5972 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2771 2771 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 2228 1943 Size: 285 5860 XmaI C'CCGG_G Cuts at: 1870 3470 1870 Size: 1600 4545 XmnI GAAnn'nnTTC Cuts at: 3393 4274 3393 Size: 881 5264 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI