(Linear) (Six-base) MAPSORT of: KIEE5645.seq Check: 5181 from: 1 to: 1440 KIEE5645.orf With 182 enzymes: SgfI * December 5, 2014 13:04 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1013 1375 1440 Size: 1013 362 65 Fragments arranged by size: 1013 362 65 AleI CACnn'nnGTG Cuts at: 0 623 1440 Size: 623 817 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 358 390 1440 Size: 358 32 1050 Fragments arranged by size: 1050 358 32 AseI AT'TA_AT Cuts at: 0 857 1440 Size: 857 583 AvaI C'yCGr_G Cuts at: 0 223 1440 Size: 223 1217 BamHI G'GATC_C Cuts at: 0 997 1440 Size: 997 443 BanI G'GyrC_C Cuts at: 0 1239 1440 Size: 1239 201 BanII G_rGCy'C Cuts at: 0 1019 1145 1440 Size: 1019 126 295 Fragments arranged by size: 1019 295 126 BbeI G_GCGC'C Cuts at: 0 1243 1440 Size: 1243 197 BbsI GAAGACnn'nnnn_ Cuts at: 0 125 349 1020 1440 Size: 125 224 671 420 Fragments arranged by size: 671 420 224 125 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 733 767 1440 Size: 733 34 673 Fragments arranged by size: 733 673 34 BclI T'GATC_A Cuts at: 0 1058 1440 Size: 1058 382 BlpI GC'TnA_GC Cuts at: 0 725 1440 Size: 725 715 Bme1580I G_kGCm'C Cuts at: 0 1266 1440 Size: 1266 174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 1440 Size: 358 1082 Bpu10I CC'TnA_GC Cuts at: 0 22 1440 Size: 22 1418 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 260 1239 1440 Size: 260 979 201 Fragments arranged by size: 979 260 201 BsaBI GATnn'nnATC Cuts at: 0 242 1440 Size: 242 1198 BsaHI Gr'CG_yC Cuts at: 0 1240 1440 Size: 1240 200 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 478 508 1440 Size: 478 30 932 Fragments arranged by size: 932 478 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 502 1246 1249 1440 Size: 502 744 3 191 Fragments arranged by size: 744 502 191 3 BseYI C'CCAG_C Cuts at: 0 1330 1440 Size: 1330 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 1440 Size: 68 1372 BsiHKAI G_wGCw'C Cuts at: 0 28 620 717 1440 Size: 28 592 97 723 Fragments arranged by size: 723 592 97 28 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1335 1440 Size: 1335 105 Bsp1286I G_dGCh'C Cuts at: 0 28 620 717 1019 1145 1266 1440 Size: 28 592 97 302 126 121 174 Fragments arranged by size: 592 302 174 126 121 97 28 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 362 1408 1440 Size: 362 1046 32 Fragments arranged by size: 1046 362 32 BsrGI T'GTAC_A Cuts at: 0 303 317 1440 Size: 303 14 1123 Fragments arranged by size: 1123 303 14 BstBI TT'CG_AA Cuts at: 0 543 1440 Size: 543 897 BstYI r'GATC_y Cuts at: 0 997 1440 Size: 997 443 BtgI C'CryG_G Cuts at: 0 843 1145 1440 Size: 843 302 295 Fragments arranged by size: 843 302 295 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 395 1440 Size: 395 1045 BtsI GCAGTG_nn' Cuts at: 0 987 1440 Size: 987 453 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 990 1025 1440 Size: 990 35 415 Fragments arranged by size: 990 415 35 DrdI GACnn_nn'nnGTC Cuts at: 0 970 1440 Size: 970 470 EaeI y'GGCC_r Cuts at: 0 556 1440 Size: 556 884 EarI CTCTTCn'nnn_ Cuts at: 0 473 1333 1379 1413 1440 Size: 473 860 46 34 27 Fragments arranged by size: 860 473 46 34 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 1013 1375 1440 Size: 358 655 362 65 Fragments arranged by size: 655 362 358 65 EcoO109I rG'GnC_Cy Cuts at: 0 228 1440 Size: 228 1212 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 569 1440 Size: 569 871 HaeII r_GCGC'y Cuts at: 0 1243 1440 Size: 1243 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 60 92 478 510 1440 Size: 60 32 386 32 930 Fragments arranged by size: 930 386 60 32 32 HincII GTy'rAC Cuts at: 0 387 1440 Size: 387 1053 KasI G'GCGC_C Cuts at: 0 1239 1440 Size: 1239 201 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 528 1440 Size: 528 912 MscI TGG'CCA Cuts at: 0 558 1440 Size: 558 882 MslI CAynn'nnrTG Cuts at: 0 623 842 1440 Size: 623 219 598 Fragments arranged by size: 623 598 219 NarI GG'CG_CC Cuts at: 0 1240 1440 Size: 1240 200 NcoI C'CATG_G Cuts at: 0 843 1145 1440 Size: 843 302 295 Fragments arranged by size: 843 302 295 NspI r_CATG'y Cuts at: 0 393 1440 Size: 393 1047 PsiI TTA'TAA Cuts at: 0 422 1440 Size: 422 1018 PspXI vC'TCGA_Gb Cuts at: 0 223 1440 Size: 223 1217 PstI C_TGCA'G Cuts at: 0 1405 1440 Size: 1405 35 SapI GCTCTTCn'nnn_ Cuts at: 0 1379 1440 Size: 1379 61 SexAI A'CCwGG_T Cuts at: 0 293 1440 Size: 293 1147 SfcI C'TryA_G Cuts at: 0 1401 1440 Size: 1401 39 SfoI GGC'GCC Cuts at: 0 1241 1440 Size: 1241 199 SmlI C'TyrA_G Cuts at: 0 223 275 1254 1440 Size: 223 52 979 186 Fragments arranged by size: 979 223 186 52 StyI C'CwwG_G Cuts at: 0 843 1145 1440 Size: 843 302 295 Fragments arranged by size: 843 302 295 TatI w'GTAC_w Cuts at: 0 303 317 1309 1440 Size: 303 14 992 131 Fragments arranged by size: 992 303 131 14 XbaI T'CTAG_A Cuts at: 0 1303 1440 Size: 1303 137 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 766 1440 Size: 766 674 XhoI C'TCGA_G Cuts at: 0 223 1440 Size: 223 1217 XmnI GAAnn'nnTTC Cuts at: 0 1388 1440 Size: 1388 52 Enzymes that do cut: AcuI AleI AloI AseI AvaI BamHI BanI BanII BbeI BbsI BcgI BclI BlpI Bme1580I BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrGI BstBI BstYI BtgI BtgZI BtsI CspCI DrdI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI MslI NarI NcoI NspI PsiI PspXI PstI SapI SexAI SfcI SfoI SmlI StyI TatI XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AscI AsiSI AvrII BaeI BbvCI BciVI BfrBI BglI BglII BmgBI BmrI BmtI BplI BsaI BsaAI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I ClaI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XmaI ZraI