################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HpaI KasI KspI MaeI MamI MfeI MflI MluI MroI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 1356 GGTACC G^GTACC AcyI 40 GGCGTC G[GA]^CG[CT]C AfaI 1357 GTAC GT^AC AflIII 1163 ACGTGT A^C[GA][CT]GT Asp700I 1325 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1356 GGTACC G^GTACC BalI 1055 TGGCCA TGG^CCA BbsI 73 GTCTTC GAAGAC(2/6) BpuAI 73 GTCTTC GAAGAC(2/6) BsaAI 1162 TACGTG [CT]AC^GT[GA] BsaHI 40 GGCGTC G[GA]^CG[CT]C BsaI 161 GGTCTC GGTCTC(1/5) BseRI 328 GAGGAG GAGGAG(10/8) BsmI 605 GAATGC GAATGC(1/-1) BsrBI 1176 GAGCGG CCGCTC(-3/-3) BstBI 808 TTCGAA TT^CGAA DpnII 852 GATC ^GATC DraII 4 AGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 1055 TGGCCA [CT]^GGCC[GA] EcoNI 440 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 4 AGGTCCT [GA]G^G[ACGT]CC[CT] FauI 1066 CCCGC CCCGC(4/6) Hin1I 40 GGCGTC G[GA]^CG[CT]C HincII 630 GTCAAC GT[CT]^[GA]AC HindII 630 GTCAAC GT[CT]^[GA]AC HindIII 1326 AAGCTT A^AGCTT KpnI 1356 GGTACC GGTAC^C MboI 852 GATC ^GATC MluNI 1055 TGGCCA TGG^CCA MscI 1055 TGGCCA TGG^CCA MslI 458 CACAATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 395 CCATGG C^CATGG NdeII 852 GATC ^GATC NspV 808 TTCGAA TT^CGAA PflMI 967 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 4 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 1045 CAGCTG CAG^CTG RsaI 1357 GTAC GT^AC Sau3AI 852 GATC ^GATC SfcI 166 CTACAG C^T[GA][CT]AG SfuI 808 TTCGAA TT^CGAA StuI 819 AGGCCT AGG^CCT TaqI 809 TCGA T^CGA TthHB8I 809 TCGA T^CGA Van91I 967 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1325 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 169 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1042 CAGCAGCTG AocI 676 CCTAAGG CC^T[ACGT]AGG 691 CCTCAGG AspHI 985 GAGCTC G[AT]GC[AT]^C 1181 GAGCTC AspI 534 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1254 GACCCAGTC BglI 123 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 310 GCCAACCAGGC BsaWI 289 ACCGGA [AT]^CCGG[AT] 1079 TCCGGT BsiHKAI 985 GAGCTC G[AT]GC[AT]^C 1181 GAGCTC BstAPI 600 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1041 GCAGCAGCTGC Bsu36I 676 CCTAAGG CC^T[ACGT]AGG 691 CCTCAGG DsaI 395 CCATGG C^C[GA][CT]GG 1097 CCGTGG Eco81I 676 CCTAAGG CC^T[ACGT]AGG 691 CCTCAGG MaeII 1163 ACGT A^CGT 1295 ACGT MspA1I 1045 CAGCTG C[AC]G^C[GT]G 1067 CCGCTG PflFI 534 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1254 GACCCAGTC SacI 985 GAGCTC GAGCT^C 1181 GAGCTC SapI 372 GAAGAGC GCTCTTC(1/4) 468 GAAGAGC Tth111I 534 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1254 GACCCAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AluI AvaII BanI BanII BbvI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeIII MboII MnlI MseI MspI MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I TspRI